AS  Vol.3 No.7 , November 2012
Bioinformatics analysis of metallothionein gene from Agaricus bisporus, Ganoderma lucidum, Taiwanofungus camphorates and Paxillus involutus
Abstract: Metallothionein (MT) gene has an important role in the detoxification of toxic metals especially some heavy metals. Based on the published sequences of MT genes from Agaricus bisporus, Garnoderma lucidum, Taiwanofungus camphoratus and Paxillus involutus as a type of the edible or medical mushroom in NCBI database, their molecular structures, physiological functions and evolutionary relationship were analyzed using the bioinformatics methods. Results showed that AbMT, GlMT, TcMT and PiMT genes contained complete open reading frame (ORF) and their theoretical points of the last three encoding proteins were higher than 7.0. AbMT, GlMT, TcMT and PiMT were relatively rich in random coil and extended strand, but transmembrane helices and signal peptides were not found. 4 MTs were mainly localized in cell nucleus (over 60%) and their cellular functions might have some relation to central intermediary metabolism. Multiple sequence alignment indicated relatively high identity (more than 52%) and short genetic distances (lower than 0.900) among 4 MT nucleotide sequences. Abundant genetic diversity and strong codon bias were found based on the halotype diversity, average number of nucleotide differences, nucleotide diversity, effective number of codons, codon bias index and scaled chisquare. Simultaneously, we deduced that 4 MT genes during molecular evolution were under positive selection. The present study might provide basis for further investigation of MTs molecular mechanisms and genetic laws.
Cite this paper: Qiang, C. , Zhou, B. , Yang, X. , Wei, F. , Lu, Z. , Wang, S. , Qin, Y. and Wang, S. (2012) Bioinformatics analysis of metallothionein gene from Agaricus bisporus, Ganoderma lucidum, Taiwanofungus camphorates and Paxillus involutus. Agricultural Sciences, 3, 900-904. doi: 10.4236/as.2012.37109.

[1]   Hamer, D.H. (1986) Metallothionein. Annual Review of Biochemistry, 55, 913-951. doi:10.1146/annurev.biochem.55.1.913

[2]   Cherian, M.G., Jayasurya, A. and Bay, B.H. (2003) Metallothioneins in human tumors and potential roles in carcinogenesis. Mutation Research, 533, 201-209. doi:10.1016/j.mrfmmm.2003.07.013

[3]   Ren, H.M., Xu, M.X., He, P.F., Muto, N., Itoh, N., Tanaka, K., Xing, J. and Chu, M.M. (2006) Cloning of crucian carp (Carassius cuvieri) metallothionein-II gene and characterization of its gene promoter region. Biochemical and Biophysical Research Communications, 342, 1297-1304. doi:10.1016/j.bbrc.2006.02.082

[4]   Huang, G.Y. and Wang, Y.S. (2009) Expression analysis of type 2 metallothionein gene in mangrove species (Bruguiera gymnorrhiza) under heavy metal stress. Chemosphere, 77, 1026-1029. doi:10.1016/j.chemosphere.2009.07.073

[5]   Huang, G.Y. and Wang, Y.S. (2010) Expression and characterization analysis of type 2 metallothionein from grey mangrove species (Avicennia marina) in response to heavy metal stress. Aquatic Toxicology, 99, 86-92. doi:10.1016/j.aquatox.2010.04.004

[6]   Mejáre, M. and Bülow, L. (2001) Metal-binding proteins and peptides in bioremediation and phytoremediation of heavy metals. Trends in Biotechnology, 19, 67-73. doi:10.1016/S0167-7799(00)01534-1

[7]   Schroeder, J.J. and Cousins, R.J. (1990) Interleukin 6 regulates metallothionein gene expression and zinc metabolism in hepatocyte monolayer cultures. Proceedings of the National Academy of Sciences, 87, 3137-3141. doi:10.1073/pnas.87.8.3137

[8]   Haq, F., Mahoney, M. and Koropatnick, J. (2003) Signaling events for metallothionein induction. Mutation Research, 533, 211-226. doi:10.1016/j.mrfmmm.2003.07.014

[9]   Akashi, K., Nishimura, N., Ishida, Y. and Yokota, A. (2004) Potent hydroxyl radical-scavenging activity of drough-induced type-2 metallothionein in wild watermelon. Biochemical and Biophysical Research Communications, 323, 72-78. doi:10.1016/j.bbrc.2004.08.056

[10]   Zhou, G.K., Xu, Y.F., Li, J., Yang, L.Y. and Liu, J.Y. (2006) Molecular Analyses of the Metallothionein Gene Family in Rice (Oryza sativa L.). Journal of Biochemistry and Molecular Biology, 39, 595-606. doi:10.5483/BMBRep.2006.39.5.595

[11]   Cai, L., Koropatnick, J. and Cherian, M.G. (1995) Metallothionein protects DNA from copper-induced but not iron-induced cleavage in vitro. Chemistry & Biology, 96, 143-155. doi:10.1016/0009-2797(94)03585-V

[12]   Hafiz, F., Begum, M., Parveen, S., Nessa, Z. and Azad, A.K.M. (2003) Study of edible mushroom grown on Eucalyptus Camaldulensis trunk and under the soil of Albizzia Procera. Pakistan Journal of Nutrition, 2, 279-282. doi:10.3923/pjn.2003.279.282

[13]   Ita, B.N., Ebong, G.A., Essien, J.P. and Eduok, S.I. (2008) Bioaccumulation potential of heavy metals in edible fungal sporocarps from the Niger Delta Region of Nigeria. Pakistan Journal of Nutrition, 7, 93-97. doi:10.3923/pjn.2008.93.97

[14]   Das, N. (2005) Heavy metal biosorption by mushrooms. Natural Product Radiance, 4, 454-459.

[15]   Das, N., Vimala, R. and Karthika, R. (2008) Biosorption of heavy metal—An overview. Indian Journal of Biotechnology, 7, 159-169.

[16]   Zhu, F.K., Qu, L., Fan, W.X., Qiao, M.Y., Hao, H.L. and Wang, X.J. (2011) Assessment of heavy metals in some wild edible mushrooms collected from Yunnan Province, China. Environmental Monitoring and Assessment, 179, 191-199. doi:10.1007/s10661-010-1728-5

[17]   Fuminori, O. (2001) Molecular mechanism of the metallothionein gene expression mediated by metal-responsive transcription factor 1. Journal of Health Science, 47, 513- 519. doi:10.1248/jhs.47.513

[18]   Jiang, Y., Tan, X.F., Zhang, D.Q. and Chen, H.P. (2009) Cloning and sequence analysis of a metallothionein gene from Camellia oleifera. Acta Agriculturae Universitati, 31, 699-705.

[19]   Liu, W.Q., Ni, D.J., Song, L.S., Wu, L.T., Xu, W. and Kong, X.Y. (2006) Cloning and characterization of a metallothionein gene in bay scallop, Argopecten irradians. Oceanologia et Limnologia Sinica, 37, 444-449.

[20]   Zhang, Y., Yang, C.P. and Wang, Y.C. (2007) Cloning and sequence analysis of metallothionein gene from Tamarix androssowii. Bulletin of Botanical Research, 27, 293-296.

[21]   Zhang, D., Ren, G.D., Tang, T., Dong, X.Y. and Liu, F.S. (2010) Cloning, prokaryotic expression and activity detection of the metallothionein gene in Musca domestica. Acta Entomologica Sinica, 53, 379-384.

[22]   Yuan, Y.M., Wang, G.L. and Li, J.L. (2009) Cloning and sequence analysis of metallothionein gene in Hyriopsis cumingii. Chinese Journal of Zoology, 44, 98-104.

[23]   Liu, Y., Chen, S.H., Yin, H.B., Li, L.X., Zhao, J.Q., Zhang, Y., Han, H.L. and Guo, S.L. (2011) Cloning and analysis of metallothionein gene from Limonium sinense Kuntze. Journal of Yantai University (Natural Science and Engineering Edition), 24, 121-125.

[24]   Lei, W., Zhang, J.D. and Qiao, A.M. (2011) Bioinformatics data mining on UDP-glucose: Flavonoid 7-O-glucosyltransferase (UBGAT) genes and their encoding proteins in two plants of genus Scutellaria. African Journal of Biotechnology, 10, 4339-4346.

[25]   Xie, D.Y., Zhu, W., Wu, Z.J., Lin, Q.Y. and Xie, L.H. (2007) Cloning and sequence analysis of metallothionein gene from Garnoderma lucidum. Chinese Agricultural Science Bulletin, 23, 87-90.

[26]   Lin, C.H., John, J.A.C., Ou, L.W., Chen, J.C., Lin, C.H. and Chang, C.Y. (2004) Cloning and characterization of metallothionein gene in ayu Plecoglossus altivelis. Aquatic Toxicology, 66, 111-124.

[27]   Guo, Z.P. (1993) Introduction to population genetics. Agricultural Press, Beijing, 298-332.

[28]   Qiang, C.K., Feng, W.J., Zhao, H., Su, X.L. and Zhou, B.Y. (2010) In silico cloning, sequence analysis and electronic pattern of polyubiquitin gene in the silkworm, Bombyx mori (Lepdoptera: Bombycidae). Genomics and Applied Biology, 29, 783-790.