ABB  Vol.1 No.3 , August 2010
Properties of crude laccase from Trametes versicolor produced by solid-substrate fermentation
ABSTRACT
A low-cost process for the production of laccases is necessary for a sustainable enzymatic wastewater treatment. Therefore, it is necessary to establish an easy and low-cost procedure for the production of laccase. In the present study the properties of crude laccase from Trametes versicolor produced by solid-substrate fermentation is investigated. The application of the enzyme for dye decolorization is also studied. Crude laccase from the studied culture established maximal activity at 45ºC. The enzyme retained over 90% of its activity in the temperature range 40- 47ºC and pH 4.5. The kinetic constants of the crude enzyme was also determined. In the presence of KCl, NaCl, CaCl2, MnSO4 and MgSO4, laccase demonstra- ted high stability—over 50% of its initial activity was still retained after 4-month incubation. Complete loss of enzymatic activity was observed in the presence of CuCl2, FeCl2, FeCl3 and NaN3 after 30 min of incubation. 100% decolorization by investigated crude laccase was completed in the case of Indigo Carmine for 4 h, Remazol Brilliant Blue R—for 6 h, Orange II— for 48 h and Congo Red—for 13 d.

Cite this paper
nullStoilova, I. , Krastanov, A. and Stanchev, V. (2010) Properties of crude laccase from Trametes versicolor produced by solid-substrate fermentation. Advances in Bioscience and Biotechnology, 1, 208-215. doi: 10.4236/abb.2010.13029.
References
[1]   Thurston, C.F. (1994) The structure and function of fungal laccases. Microbiology, 40, 19-26.

[2]   Gianfreda, L., Xu, F. and Bollag, J. (1999) Laccases: A useful group of oxidoreductive enzymes. Bioremediation Journal, 3(1), 1-25.

[3]   Call, H.P. and Mucke, I. (1997) History, overview and applications of mediated lignolytic systems, especially laccase-mediator-systems (Lignozym(R)-process). Jour- nal of Biotechnology, 53(2), 163-202.

[4]   Bulter, T., Alcalde, M., Sieber, V., Meinhold, P., Schlacht- bauer, C. and Arnold, F.H. (2003) Functional expression of a fungal laccase in Saccharomyces cerevisiae by directed evolution. Applied and Environment Microbiology, 69(2), 987-995.

[5]   Eggert, C., Temp, U. and Eriksson, K.E.L. (1996) Laccase-producing white-rot fungus lacking lignin peroxidase and manganese peroxidase. ACS Symposium Series, 655, 130-150.

[6]   Solomon, E.I., Sundaram, U.M. and Machonkin, T.E. (1996) Multicopper oxidases and oxygenases. Chemistry Reviews, 96(7), 2563-2605.

[7]   Schlosser, D., Grey, R. and Fritsche, W. (1997) Patterns of ligninolytic enzymes in Trametes versicolor. Distri- bution of extra- and intracellular enzyme activities during cultivation on glucose, wheat straw and beech wood. Applied Microbiology and Biotechnology, 47, 412-418.

[8]   Swamy, J. and Ramsay, J.A. (1999) Effects of Mn2+ and NH4+ concentrations on laccase and manganese peroxidase production and Amaranth decoloration by Trametes versicolor. Applied Microbiology and Biotechnology, 51, 391-396.

[9]   Pandey, A., Selvakumar, P., Soccol, C.R. and Nigam P. (1999) Solid state fermentation for the production of industrial enzymes. Current Science, 77(1), 149-162.

[10]   Murthy, M.V.R., Karanth, N.G. and Raghava Rao, K.S.M.S. (1993) Biochemical engineering aspects of solid-state fermentation. Advances in Applied Microbiology, 38, 99-147.

[11]   Ashakumary, L., Selvakumar, P.S. and Pandey, A. (1994) Column fermentor for solid state fermentation. In: Pandеy, A., Ed., Solid State Fermentation, Wiley Eastern Limited/Newage International Publishers, 33.

[12]   Viniegra-González, G., Favela-Torres, E., Aguilar, C., Romero-Gómez, J., Díaz-Godínez, G. and Augur, C. (2003) Advantages of fungal enzyme production in solid state over liquid fermentation systems. Biochemical Engineering, 13(2-3), 157-167.

[13]   Gianfreda, L., Xu, F. and Bollag, J.M. (1999) Enzymatic oxidative transformation of chlorophenol mixtures. Journal of Environmental Quality, 32(1), 63-69.

[14]   Xu, F. (1999) Laccase. In: Michael, C.F. and Stephen, W.D., Ed., Encyclopedia of Bioprocess Technology: Fer- mentation, Biocatalysis, and Bioseparation, Wiley, NY, 1545-1554.

[15]   Yaropolov, A.I., Skorobogat’ko, O.V., Vartanov, S.S. and Varfolomeyev, S.D. (1994) Laccase: Properties, catalytic mechanism, and applicability. Applied Biochemistry and Biotechnology, 49(3), 257-280.

[16]   Rodriguez, C.S. and Herrera, J.L.T. (2006) Industrial and biotechnological applications of laccases: A review. Biotechnology Advances, 24(5), 500-513.

[17]   Camarero, S., Lbarra, D., Marinez, A.T., Romero, J., Gutierrez, A. and Del Rio, J.C. (2007) Paper pulp delignification using laccase and natural mediators. Enzyme and Microbial Technology, 40(5), 1264-1271.

[18]   Myasoedova, N.M., Chernykh, A.M., Psurtseva, N.V., Belova, N.V. and Golovleva, L.A. (2008) New efficient producers of fungal laccases. Applied Biochemistry and Microbiology, 44(1), 73-77.

[19]   Marbach, I., Harel, E. and Mayer, A.M. (1985) Pectin, a second product for laccase production by Botrytis cinerea. Phytochemistry, 24(11), 2559-2561.

[20]   Johanes, C. and Majcherczyk, A. (2000) Laccase activity test and laccase inhibitors. Journal of Biotechnology, 78(2), 193-199

[21]   Mathews, J. and Fink, K. (2001) Numerical methods using MATLAB. Prentice Hall, Upper Saddle River, NJ.

[22]   Stanchev, V., Stoilova, I. and Krastanov, A. (2008) Biodegradation dynamics of high catechol concentrations by Aspergillus awamori. Journal of Hazardous Materials, 154(1-3), 396-402.

[23]   Zouari-Mechichi, H., Mechichi, T., Dhouib, A., Sayadi, S., Martínez, A.T. and Martínez, M.J. (2006) Laccase purification and characterization from Trametes trogii isolated in Tunisia: decolorization of textile dyes by the purified enzyme. Enzyme and Microbial Technology, 39(1), 141-148.

[24]   Litthauer, D., Vuuren, M.J., Tonder, A. and Wolfaardt, F. (2007) Purification and kinetics of a thermostable laccase from Pycnoporus sanguineus (SCC 108). Enzyme and Microbial Technology, 40(4), 563-568.

[25]   Palonen, H., Saloheimo, M., Viikari, L. and Kruus, K. (2003) Purification, characterization and sequence analysis of a laccase from the ascomycete Mauginiella sp. Enztme and Microbial Technology, 33(6), 854-862.

[26]   Jordaan, J., Pletschke, B.I. and Leukes, W.D. (2004) Purification and characterization of a thermostable laccase from an unidentified basidiomycete. Enzyme and Microbial Technology, 34(6), 549-554.

[27]   Banat, I.M., Nigam, P., Singh, D. and Marchant, R. (1996) Microbial decolourization of textile-dye-containing effluents: A review. Bioresource Technology, 58(3), 217- 227.

[28]   Novotny, C., Rawal, B., Bhatt, M., Patel, M., Sasek, V. and Molotoris, H.P. (2001) Capacity of Irpex lacteus and Pleurotus ostreatus for decolorization of chemically different dyes. Journal of Biotechnology, 89(2-3), 113-122.

[29]   Soares, G.M.B., Pessoa de Amorim, M.T. and Costa-Ferreira, M. (2001) Use of laccase together with redox mediators to decolourize Remazol Brilliant Blue R. Journal of Biotechnology, 89(2-3), 123-129.

[30]   Li, K., Xu, F. and Eriksson, K.L. (1999) Comparison of fungal laccases and redox mediators in oxidation of a non-phenolic lignin model compound. Applied and Environmental Microbiology, 65(6), 2654-2660.

[31]   Nyanhongo, G.S., Gomes, J., Gübitz, G.M., Zvangya, R., Read, J. and Steiner, W. (2002) Decolourisation of textile dyes by laccases from a newly isolated strain of Trametes modesta. Water Research, 36(6), 1449-1456.

[32]   Birhanli, E. and Yesilada, O. (2006) Increased production of laccase by pellets of Funalia trodii ATTC 200800 and Trametes versicolor ATCC 200801 in repeated-batch mod. Enzyme and Microbial Technology, 39(6), 1286- 1293.

[33]   Baldrian, P. and ?najdr, J. (2006) Production of lignolytic enzymes by litter-decomposing fungi and their ability to decolorize synthetic dyes. Enzyme and Microbial Technology, 39(5), 1023-1029.

 
 
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