Health  Vol.4 No.9 A , September 2012
Selective changes in the α-adrenoceptor-mediated contraction in the senescent rat urinary bladder
Abstract: The urinary bladder is innervated and functionally regulated by the autonomic nervous system. In order to elucidate the mechanism of functional changes in aged rat urinary bladder, we studied the influence of senescence on, 1) the α-adrenergic contractile response to phenylephrine in the urinary bladder body and trigone, 2) the muscarinic contractile response to carbachol in the body and trigone. The binding characteristics of [3H]quinuclidinyl benzilate (QNB) to muscarinic cholinoceptors were compared in young and aged bladder. Bladders from young (2 - 3 month-old) and aged (27 month-old) male Fischer 344 rats were isolated, cut into strips and mounted in the organ bath, then the developed tension was recorded. Histologically, the aged bladder did not show pathologic changes such as inflammation and hypertrophy. Carbachol-induced contraction in aged rat bladder was identical to that obtained in young rat. In the receptor binding assay, [3H]QNB maximal binding capacity and Kd value were not significantly changed in aged bladder. In contrast, a selective α-adrenergic agonist phenylephrine, elicited greater contractions both in the aged body and trigone than those in young rats. The augmentation of α-adrenoceptor-mediated contractions in aged bladder may induce urinary dysfunction such as voiding difficulty.
Cite this paper: Aita, T. , Ishihata, A. , Yamada, A. and Katano, Y. (2012) Selective changes in the α-adrenoceptor-mediated contraction in the senescent rat urinary bladder. Health, 4, 743-749. doi: 10.4236/health.2012.429115.

[1]   Okamura, K., Usami, T., Nagahama, K., Maruyama, S. and Mizuta, E. (2002) “Quality of life” assessment of urination in elderly Japanese men and women with some medical problems using international prostate symptom score and king’s health questionnaire. European Urology, 41, 411-419. doi:10.1016/S0302-2838(02)00061-1

[2]   Mathias, H.D., Pfisterer, M.H.D., Griffiths, D.J., Schaefer, W. and Resnick, N.M. (2006) The effect of age on lower urinary tract function: A study in women. Journal of American Geriatrics Society, 54, 405-412. doi:10.1111/j.1532-5415.2005.00613.x

[3]   Anderson, K.E. and Amer, A. (2004) Urinary bladder con-traction and relaxation: Physiology and pathophysiology. Physiological Review, 84, 935-986. doi:10.1152/physrev.00038.2003

[4]   Anderson, K.E. and Wein, A.J. (2004) Pharmacology of the lower urinary tract: Basis for current and future treatments of urinary incontinence. Pharmacological Review, 56, 581-631. doi:10.1124/pr.56.4.4

[5]   Lowry, O.H., Resevrough, N.J., Farr, A.L. and Tandall, R.J. (1951) Protein measurement with the folin phenol reagent. Journal of Biological Chemistry, 193, 265-275.

[6]   Kolta, M.G., Wallace, L.J. and Gerald, M.C. (1984) Age-related changes in sensitivity of rat urinary bladder to autonomic agents. Mechanism of Ageing and Development, 27, 183-188. doi:10.1016/0047-6374(84)90043-5

[7]   Ordway, G.A., Esbenshade, T.A., Kolta, M.G., Gerald, M.C., Wallace and L.J. (1986) Effect of age on cholinergic muscarinic responsiveness and receptors in the rat urinary bladder. Journal of Urology, 136, 492-496.

[8]   Suzuki, Y., Moriyama, N., Kanada, A., Okaya, Y., Kawabe, K. and Aisaka, K. (1999) The role of a1L-adrenoceptor in rat urinary bladder: Comparison between young adult and aged rats. Life Science, 65, 2553-2559. doi:10.1016/S0024-3205(99)00524-X

[9]   Saito, M., Kondo, A. and Gotoh, M. (1991) Agererated changes in the rat detrusor muscle: The contractile response to inorganic ions. Journal of Urology, 146, 891-894.

[10]   Munro, D.D. and Wendt, I.R. (1993) Contractile and metabolic properties of longitudinal smooth muscle from rat urinary bladder and the effects of aging. Journal of Urology, 150, 529-536.

[11]   Pagala, M.K., Tetsoti, L., Nagpal, D. and Wise, G.J. (2001) Aging effects on contractility of longitudinal and circular detrusor and trigone of rat bladder. Journal of Urology, 166, 721-727. doi:10.1097/00005392-200108000-00100

[12]   Caulfield, M.P. and Birdsall, N.J.M. (1998) International union of pharmacology: XVII. Classification of muscarinic acetylcholine receptors. Pharmacological Review, 50, 279-290.

[13]   Wang, P., Luthin, G.R. and Ruggieri, M.R. (1995) Muscarinic acetylcholine receptor subtypes mediating urinary bladder contractility and coupling to GTP binding proteins. Journal of Parmacology and Experimental Therapeutics, 273, 959-966.

[14]   Chiarini, A., Budriesi, R., Bolognesi, ML., Minarini, A. and Melchiorre, C. (1995) In vitro characterization of tripitramine, a polymethylene tetraamine displaying high selectivity and affinity for muscarinic M2 receptors. British Journal of Pharmacology, 114, 1507-1517. doi:10.1111/j.1476-5381.1995.tb13378.x

[15]   Hegde, S.S. and Eglen, R.M. (1999) Muscarinic receptor subtypes modulating smooth muscle contractility in the urinary bladder. Life Science, 64, 419-428. doi:10.1016/S0024-3205(98)00581-5

[16]   Braverman, A.S., Luthin, G.R. and Ruggieri, MR. (1998) M2 muscarinic receptor contributes to contraction of the denervated rat urinary bladder. American Journal of Physiology, 275, R1654-R1660.

[17]   Hayes, E.E., McConnell, J.A. and Benson, G.S. (1983) The effect of aging on cholinergic receptor binding in the rat urinary bladder. Neurourology and Urodynamics, 2, 311-315. doi:10.1002/nau.1930020407

[18]   Ordway, G.A., Kolta, M.G., Gerald, M.C. and Wallace, L.J. (1986) Agerelated change in alpha-adrenergic responsive-ness of the urinary bladder of the rat is regionally specific. Neuropharmacology, 25, 1335-1340. doi:10.1016/0028-3908(86)90105-X

[19]   Marshall, I., Burt, R.P. and Chapple, C.R. (1995) Noradrena-line contractions of human prostate mediated by alpha1A-(alpha1C-) adrenoceptor subtype. British Journal of Pharmacology, 115, 781-786. doi:10.1111/j.1476-5381.1995.tb15001.x

[20]   Nasu, K., Miriyama, N. and Fukusawa, R. (1998) Quantification and distribution of a1-adrenoceptor subtype mRNAs in human proximal urethra. British Journal of Pharmacology, 123, 1289-1293. doi:10.1038/sj.bjp.0701731

[21]   Restorick, J.M. and Mundy, A.R. (1989) The density of cholinergic and alpha and beta adrenergic receptors in the normal and hyper-reflexic human detrusor. British Journal of Urology, 63, 32-35. doi:10.1111/j.1464-410X.1989.tb05120.x

[22]   Dmitrieva, N., Zhang, G. and Nagabukuro, H. (2008) Increased alpha1D adrenergic receptor activity and protein expression in the urinary bladder of aged rats. World Journal of Urology, 26, 649-655. doi:10.1007/s00345-008-0292-x

[23]   Lluel, P., Palea, S., Barras, M., Grandadam, F., Heudes, D., Bruneval, P., Corman, B. and Martin, D.J. (2000) Functional and morphological modifications of the urinary bladder in aging female rats. American Journal of Physiology, 278, R964-R972.

[24]   Lepor, H., Sunarvadi, I., Hartanto, V. and Shapiro, E. (1992) Quantitative morphometry of the adult human bladder. Journal of Urology, 148, 414-417.