Health  Vol.2 No.7 , July 2010
Dynamic analysis of lymphocyte subsets of peripheral blood in patients with acute self-limited hepatitis B
ABSTRACT
Purpose: To investigate dynamic changes and significance of lymphocyte subsets (T lymphocytes, B lymphocytes, NK cells and T cell subsets) of peripheral blood in patients with acute self-limited hepatitis B (AHB). Methods: Immune cells of peripheral blood were compared among 17 cases of self-limited acute hepatitis B patients, 36 patients with chronic hepatitis B (CHB) and 32 healthy controls by flow cytometry (FCM). CD4+/CD8+ was monitored dynamically, meanwhile relations between T lymphocyte subsets and ALT and clearance of HBV DNA were explored. Results: Dynamic changes of lymphocyte subsets were found in AHB, the level of CD3+T cells was significantly higher compared to CHB group and healthy control group. Frequencies of CD3+CD4+ T cells in the third and fourth week and CD4+/CD8+ in the second week were higher compared to other groups. Frequ- ency of NK cells was low and was significantly lower compared to other groups in the third week specially. It was showed that CD4+/CD8+ was low followed by high abnormal ALT during early stage by dynamic monitoring of CD4+/CD8+, and CD4+/CD8+ was increasing accompanied by normal ALT set by set, but CD4+/CD8+ had no significant relation to ALT and HBV DNA. Conclusion: Immune status of AHB, compared to CHB and healthy controls, was significantly different and dynamic changes of lymphocyte sub- sets may be related to progress of disease.

Cite this paper
nullLiu, B. , Li, J. , Han, Y. , Liu, Y. , Kong, L. , Cao, Y. and Huang, Z. (2010) Dynamic analysis of lymphocyte subsets of peripheral blood in patients with acute self-limited hepatitis B. Health, 2, 736-741. doi: 10.4236/health.2010.27112.
References
[1]   World Health Organization: Department of Communicable diseases surveillance and response. (2004) Hepatitis B, WHO Fact Sheets, http://www.who.int

[2]   Robert, T., Stefan, W. and Carola, S. (2003) CD8+T cell mediate viral clearance and disease pathogenesis during acute hepatitis B virus infection. Journal of Virology, 77(1), 68-86.

[3]   You, J., Zhuang, L. and Zhang, Y.F. (2009) Peripheral T-lymphocyte subpopulations in different clinical stages of chronic HBV infection correlate with HBV load. World Journal of Biological Chemistry, 15(27), 3382- 3393.

[4]   Tian, Y., Qiu, Z.F. and Li, T.S. (2005) Difference and significance of peripheral blood T-lymphocyte subsets in patients with chronic hepatitis B and asymptomatic HBV carriers. National Medical Journal of China, 85(47), 3354-3358.

[5]   Urbani, S., Boni, C. and Amadei, B., (2005) Acute phase HBV-specific T cell responses associated with HBV persistence after HBV/HCV co-infection. Hepatology, 41(4), 826-831.

[6]   Chinese Society of Infectious Disease and Parasitology and Chinese Society of Hepatology of Chinese Medical Association. (2001)The program of prevention and cure for viral hepatitis. Chinese Journal of Infectious Disease, 19(1), 56-62.

[7]   Chinese Society of Hepatology and Infectious Disease. The guidelines of prevention and cure for chronic hepatitis B. (2005) Chinese Journal of Hepatology, 13(12), 881- 891.

[8]   Han, Y.P., Liu, Y. and Li, J. (2005) Study on the difference between the lymphocyte subsets of liver tissue and peripheral blood in liver disease patients. Labotary Medicine, 20(5), 448-451.

[9]   Bertoletti, A. and Gehring, A.J. (2006) The immune response during hepatitis B virus infection. Journal of General Virology, 87(6), 1439-1449.

[10]   Urbani, S., Boni, C. and Amadei, B. (2005) Acute phase HBV-specific T cell responses associated with HBV persistence after HBV/HCV co-infection. Hepatology, 41(4), 826-831.

[11]   Vassilopoulos, D., Rapti, I. and Nilolaou, M. (2008) Cellular immune responses in hepatitis B virus e antigen negative chronic hepatitis B. Journal of Viral Hepatitis, 15(11), 817-826.

[12]   Brian, D.L. and Jeff, A. (1999) Altered helper t lymphocyte function associated with chronic hepatitis b virus infection and its role in response to therapeutic vaccination in humans [J]. Immunol, 162(5), 3088-3095.

[13]   Ying, Y., Zhang, Y.H. and Zhang, L.C. (2002) Significance of the changes of the levels of TNF-α and sIl-2R and CD4/CD8 in patients with hepatitis B. Immunological Journal, 18(1), 74.

[14]   You, J., Zhuang, L. and Chen, H.Y. (2007) Relationship between variarations in peripheral T lymphocyte subsets and viral replication levels in Chinese chronic HBV carriers with normal liver function tests. World Chinese Journal of Digestology, 15(35), 3722-3727.

[15]   Rehermann and Nascimbeni, M, (2005) Immunology of hepatitis B virus and hepatitis C virus infection. Nature Reviews Immunology, 5(3), 215-229.

[16]   Moretta, L., Bottino, C., Pende, D., Vitale, M., Mingari, M.C. and Moretta, A. (2005) Human natural killer cells: Molecular mechanisms controlling NK cell activation and tumor cell lysis. Immunology Letters, 100(1), 7-13.

 
 
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