ABSTRACT Objects: To investigate the pathogenesis of amyloid presented in uterine leiomyoma. Methods: 36 uterine leiomyoma patients were recruited and divided into two groups according to Congo red staining results. 6 cases are Congo red staining-positive, and 30 cases Congo red staining-negative which represented amyloid positive and amyloid negative respectively. All patients’ serum total protein (TP), albumin (Alb) and prealbumin (PA) levels were measured as well as blood hemoglobin (Hb), cell counts of white blood cell (WBC), neutrophils (NEU) and lymphocyte (LYM). Glycogen in tissue was compared between amyloid accumulated and amyloid negative sections with periodic acid schiff staining (PAS) in leiomyoma patients. Results: All of blood Hb concentration, WBC, NEU and LYM have not been found significant differences between two groups. Also no obvious infiltration of inflammatory cells was observed in tissue with amyloid deposition in uterine leiomyoma patients. And levels of TP, Alb and prealbumin have not been found significant differences between two groups. The amyloid was negative in leiomyoma entity cells range by Congo red staining, while small blood vessels in myoma tissues were positively detected with high rate. Amyloid was found in normal tissue around myoma as well as in blood vessel of pseudo-capsule. Increased PAS-positive material induced by leiomyoma was not correlated with amyloid deposition. Conclusions: Metabolic changes in the setting of functional alterations of cell in local microenvironment with uterine leiomyoma, may be related to the amyloid deposition.
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Liu, J. , Zhai, F. , Ge, P. , Lu, J. , Qin, Y. and Sun, X. (2012) Investigation of amyloid deposition in uterine leiomyoma patients. Health, 4, 522-525. doi: 10.4236/health.2012.48083.
 Lurie, S., Piper, I., Woliovitch, I. and Glezerman, M. (2005) Age-related prevalence of sonographicaly confirmed uterinene myomas. Journal of Obstetrics and Gynaecology, 25, 42-44. doi:10.1080/01443610400024583
 Fujimoto, J., Hirose, R., Ichigo, S., Sakaguchi, H., Li, Y. and Tamaya, T. (1998) Expression of progesterone receptor form A and B mRNAs in uterine leiomyoma. Tumour Biology, 19, 126-131. doi:10.1159/000029983
 Andersen, J., DyReyes, V.M., Barbieri, R.L., Coachman, D.M. and Miksicek, R.J. (1995) Leiomyoma primary cultures have elevated transcriptional response to estrogen compared with autologous myometrial cultures. Journal of the Society for Gynecologic Investigation, 2, 542-551. doi:10.1016/1071-5576(94)00053-4
 Rogers, R., Norian, J., Malik, M., Christman, G., AbuAsab, M., Chen, F., et al. (2008) Mechanical homeostasis is altered in uterine leiomyoma. American Journal of Obstetrics and Gynecology, 198, 474.e1-11.
 Mitropoulou, T.N., Theocharis, A.D., Stagiannis, K.D. and Karamanos, N.K. (2001) Identification, quantification and fine structural characterization of glycosaminoglycans from uterine leiomyoma and normal myometrium. Biochimie, 83, 529-536.
 Sun, X.G., Hu, K.S., Song, L. and Feng, Q. (2009) Diagnosis of amyloidosis. Journal of Chinese Modern Medicine, 6, 292-295.
 Zhang, Q.H., Ren, Y.P. and Zhang, Q. (2004) 1 case of pituitary prolactin cell adenoma with amyloid-containing substances. International Journal of Clinical and Experimental Pathology, 20, 511-512.
 Bestard, M.O., Poveda, M.R., Ibernon, V.M., Carrera, P.M. and Grinyó, B.J.M. (2008) Systemic AA amyloidosis induced by benign neoplasms. Nefrologia, 28, 93-98.
 Wu, L., Yi, G.W. and Lei, J.X. (2000) 22 cases of pregnancy with uterine fibroids. Journal of Fourth Military Medical University, 21, 310.
 Ando, Y., Nyhlin, N., Suhr, O., Holmgren, G., Uchida, K. and Yamashita, T. (1997) Oxidative stress is found in amyloid deposits in systemic amyloidosis. Biochemical Biophysical Research Communications, 232, 497-502.
 Liu, J.P., Zheng, F., Liu, X.C., Li, X., Song, J.Y. and Sun, X.G. (2008) Chemical modification of transthyretin in familial amyloidotic polyneuropathy. Journal of Fourth Military Medical University, 29, 883-885.
 Sun, X.G., Liu, J.P., Wang, Q.Y. and Ni, E. (2007) Peroxidative modification and amyloidogenesis. Chinese Journal of Coal Industry Medicine, 10, 1345-1347.
 Connors, L.H., Jiang, Y., Budnik, M., Théberge, R., Prokaeva, T., Bodi, K.L., et al. (2007) Heterogeneity in primary structure, post-translational modifications, and germline gene usage of nine full-length amyloidogenic kappal immunoglobulin light chains. Biochemistry, 46, 14259-14271. doi:10.1021/bi7013773