Health  Vol.4 No.8 , August 2012
Antimicrobial activity of multipurpose essential oil blends
Abstract: Two types of multipurpose essential oil blends, blend11 containing eleven different essential oils and blend12 containing twelve, were tested against bacterial strains of Pseudomonas aeruginosa ATCC 9027, Serratia marcescens ATCC 13880 and Staphylococcus aureus ATCC 6538 and against the fungi, Candida albicans ATCC 10231, Aspergillus fumigatus ATCC 10894 and Fusarium solani ATCC 36031 to determine the spectrum of in vitro antimicrobial activity using aromatograms (paper disc diffusion assays). Microbial growth was decreased by multipurpose blend11 and blend12 in a similar manner. The saline control disc did not inhibit antimicrobial growth while the two blends exhibited significant zones of inhibition for all 3 bacteria and for the 3 fungi. The greatest antibacterial activity of blend11 and blend12 was exhibited with P. aeruginosa and S. marcescens followed by S. aureus. A high level of activity was associated with C. albicans and a lower level with F. solani followed by A. fumigatus. It is clearly evident from previous published studies that no single essential oil will effectively inhibit the growth of all of the organisms in our study. However, our results demonstrate that blend11 and blend12 have a broad range of inhibitory activity affecting all of the microorganisms tested.
Cite this paper: Laubach, H. , Ghanavati, S. and Whidden, G. (2012) Antimicrobial activity of multipurpose essential oil blends. Health, 4, 443-447. doi: 10.4236/health.2012.48070.

[1]   Cowan, M.M. (1999) Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12, 564-582.

[2]   Hammer, K.A., Carson, C.F. and Riley, T.V. (1999) Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology, 86, 985-990. doi:10.1046/j.1365-2672.1999.00780.x

[3]   Cimanga, K., Kambu, K., Tona, L., Apers, S., De Bruyne, T., Hermans, N., Totte, J., Pieters, L. and Vlietinck, A.J. (2002) Correlation between chemical composition and antibacterial activity of essential oils of some aromatic medicinal plants growing in the Democratic Republic of Congo. Journal of Ethnopharmacology, 79, 213-220. doi:10.1016/S0378-8741(01)00384-1

[4]   Wilkinson, J.M. and Cavanagh, H.M.A. (2005) Antibacterial activity of essential oils from Australian native plants. Phytotherapy Research, 19, 643-646. doi:10.1002/ptr.1716

[5]   Carson, C.F. and Riley, T.V. (1995) Antimicrobial activity of the major components of the essential oil of Melaleuca alternifolia. Journal of Applied Bacteriology, 78, 264-269. doi:10.1111/j.1365-2672.1995.tb05025.x

[6]   Warnke, P.H., Becker, S.T., Podschun, R., SivananthanI, S., Springer, I.N., Russo, P.A.J., Wiltfang, J., Fickenscher, H. and Sherry, E. (2009) The battle against multi-resistant strains: Renaissance of antimicrobial essential oils as a promising force to fight hospital-acquired infections. Journal of Cranio-Maxillofacial Surgery, 37, 392-397. doi:10.1016/j.jcms.2009.03.017

[7]   DigrakI, M., Ilcim, A. and Alma, M.H. (1999) Antimicrobial activities of several parts of Pinus brutia, Juniperus oxycedrus, Abies Cilicia, Cedrus libani and Pinus nigra. Phytotherapy Research, 13, 584-587. doi:10.1002/(SICI)1099-1573(199911)13:7<584::AID-PTR508>3.0.CO;2-S

[8]   Prabuseenivasan, S., Jayakumar, M. and Ignacimuthy S. (2006) In vitro antibacterial activity of some plant essential oils. BMC Complementary Alternative Medicine, 6, 39. doi:10.1186/1472-6882-6-39

[9]   Cosge, B., Turker, A., Urker, A., Ipek A., Girbuz, B. and Arslan, N. (2009) Chemical compositions and antibacterial activities of the essential oils from aerial parts and corollas of Origanum acutidens (Hand.-Mazz.) Ietswaart, an endemic species to Turkey. Molecules, 14, 1702-1712. doi:10.3390/molecules14051702

[10]   Tohidpour, A., Sarrari, M., Omidbaigi, R., Yadegar, A. and Nazemi, J. (2010) Antibacterial effect of essential oils from two medicinal plants against methicillin-resistant Staphylococcus aureus (MRSA). Phytomedicine, 17, 142-145. doi:10.1016/j.phymed.2009.05.007

[11]   Ashour, H.M. (2008) Antibacterial, antifungal, and anticancer activities of volatile oils and extracts from stems, leaves, and flowers of Eucalyptus sideroxylon and Eucalyptus torquata. Journal of Cancer Research and Therapy, 7, 399-403.

[12]   Rai, M.K., Qureshi, S. and Pandey A.K. (1999) In vitro susceptibility of opportunistic spp. to essential oils. Mycoses, 42, 97-101. doi:10.1046/j.1439-0507.1999.00267.x

[13]   Hong, E.J., Na, K.J., Choi, I.G., Choi, K.C. and Jeunge, E.B. (2004) Antibacterial and antifungal effects of essential oils from coniferous trees. Biological and Pharmaceutical Bulletin, 27, 863-866. doi:10.1248/bpb.27.863

[14]   Valimaa, A.L., Honakalampi-Hamalainen, U., Pietarinen, S., Willfor, S. and Von Wright, A. (2007) Antimicrobial and cytotoxic knot wood extracts and related pure compounds and their effects on food-associated microorganisms. International Journal of Food Microbiology, 115, 235-243. doi:10.1016/j.ijfoodmicro.2006.10.031

[15]   Sarbhoy, A.K., Varshney, J.L., Maheshwari, M.L. and Saxena, D.B. (1978) Efficacy of some essential oils and their constituents on few ubiquitous molds. Zentralblatt fur Bakteriology Naturwiss, 133, 722-725. doi:10.1016/S0323-6056(78)80079-2

[16]   Leeja, L. and Thoppil, J.E. (2007) Antimicrobial activity of methanol extract of Origanum majorana L. (sweet marjoram). Journal of Environmental Biology, 28, 145-146.

[17]   Luqman, S., Dwivedi, G.R., Darokar, M.P., Kalra, A. and Khanuja, S.P.S. (2007) Potential of rosemary oil to be used in drug-resistant infections. Alternative Therapies in Health Medicine, 13, 54-59.

[18]   Kurita, N., Miyaji, M., Kurane R. and Takahara, Y. (1981) Antifungal activity of components of essential oils. Agricultural and Biological Chemistry, 45, 945-952. doi:10.1271/bbb1961.45.945

[19]   Maruzzella, J.C. and Henry, P.A. (1958) Antimicrobial activity of perfume oils. Journal of the American Pharmaceutical Association, 47, 471-476. doi:10.1002/jps.3030470704

[20]   Yousef, R.T., Aggag, M.E. and Rawil, G.G. (1978) Evaluation of the antifungal activity of some components of volatile oils against dermatophytes. Mykosen, 21, 190-193.

[21]   Janssen, A.M., Shaeffer, J.J.C. and Baerheim S.A. (1987) Antimicrobial activity of essential oils: A 1976-86 literature review: Aspects of the test methods. Planta Medica, 53, 395-398. doi:10.1055/s-2006-962755

[22]   Mayaud, L., Carricajo, A., Zhiri, A. and Aubert, G. (2008) Comparison of bacteriostatic and bactericidal activity of 13 essential oils against strains with varying sensitivity to antibiotics. Letters in Applied Microbiology, 47, 167-173. doi:10.1111/j.1472-765X.2008.02406.x