JBPC  Vol.3 No.3 , August 2012
Molecular basis of scavenging effect of zonisamide on hydroxyl radical in vitro
ABSTRACT
Zonisamide (ZNS), a commonly used anticonvulsant, scavenged hydroxyl radicals at a clinically relevant concentration. Reactants of ZNS with hydrogen peroxide and with hydrogen peroxide plus UV irradiation, yielding hydroxyl radicals, were analyzed by the LC/MS technique. Many small fragments were found in the both reactions, suggesting that ZNS was decomposed not only by hydroxyl radicals but also by hydrogen peroxide. Furthermore, mass-fragment-grams showed that m/z: 213 (ZNS itself) was decreased markedly and m/z: 118 (may be a decomposed product by ring cleavage of ZNS) was detected specifically by treatment with hydroxyl radical. These data suggested that ZNS may react directly with free radicals.

Cite this paper
Tanaka, K. , Nanba, T. , Furubayashi, T. , Noda, Y. , James Willmore, L. and Mori, A. (2012) Molecular basis of scavenging effect of zonisamide on hydroxyl radical in vitro. Journal of Biophysical Chemistry, 3, 256-258. doi: 10.4236/jbpc.2012.33030.
References
[1]   Mori, A., Noda, Y. and Packer, L. (1998) The anticonvulsant zonisamide scavenges free radicals. Epilepsy Research, 30, 153-158. doi:10.1016/S0920-1211(97)00097-1

[2]   Asanuma, M., Miyazaki, I., Diaz-Corrales, F.J., Miyoshi, K., Ogawa, N. and Murata, M. (2008) Preventing effects of a novel anti-parkinsonian agent zonisamide on dopamine quinone formation. Neuroscience Research, 60, 106-113. doi:10.1016/j.neures.2007.10.002

[3]   Mori, A., Noda, Y., Kaneyuki, T. and Packer, L. (2000) Antiepileptic mechanism of zonisamide involves its antioxidant effect in the brain. In: Karanithi, N. and Packer, L., Eds., Microneutrients and Health: Molecular Biological Mechanisms, AOCS Press, Champaign, 236-246.

[4]   Rosen, G.M. and Rauckman, E.J. (1984) Spin trapping of superoxide and hydroxyl radicals. In: Packer, L., Ed., Methods in Enzymology, Oxygen Radicals in Biological Systems, Academic Press, San Diego, 198-209. doi:10.1016/S0076-6879(84)05026-6

[5]   Janzen, E.G. (1984) Spin trapping. In: Packer, L., Ed., Methods in Enzymology Oxygen Radicals in Biological Systems, Academic Press, San Diego, 188-198.

[6]   Ito, T., Yamaguchi, T., Miyazaki, H., Sekine, Y., Shimizu, M., Ishida, S., Yagi, K., Kakegawa, N., Seino, M. and Wada, T. (1982) Pharmacokinetic studies of AD-810, a new antiepileptic compound. Phase I trials. Arzneinmittelforsch, 32, 1581-1586.

[7]   Nakasa, H., Komiya, M., Ohmori, S., Rikihisa, T., Tiuchi, M. and Kitada, M. (1993) Characterization of human liver microsomal cytochrome P450 involved in the reductive metabolism of zonisamide. Molecular Pharmacology, 44, 216-221.

[8]   Nakasa, H., Ohmori, S. and Kitada, M. (1996) Formation of 2-sulphamoylphenol from zonisamide under aerobic conditions in rat liver microsomes. Xenobiotica, 26, 495-501. doi:10.3109/00498259609046727

[9]   Kitamura, S., Sugihara, K., Kuwasako, M. and Tatsumi, K. (1997) The role of mammalian intestinal bacteria in the reductive metabolism of zonisamide. Journal of Pharmacy and Pharmacology, 49, 253-256. doi:10.1111/j.2042-7158.1997.tb06790.x

 
 
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