AJAC  Vol.3 No.8 , August 2012
Simultaneous Quantification of Ibuprofen and Paracetamol in Tablet Formulations Using Transmission Fourier Transform Infrared Spectroscopy
Abstract: A very simple, non-destructive, inexpensive and green strategy was applied for the simultaneous determination of ibu-profen (IBP) and paracetamol (PC) using transmission Fourier Transform Infrared (FTIR) spectroscopy in tablet formulations for routine quality control laboratories. For the determination of the active pharmaceutical ingredients (API), KBr pellets containing known amount of standards and samples were used for acquisition of the FTIR spectra. The partial least squares (PLS) calibration model was developed using the spectral region from 1781 - 1683 cm-1 for IBP and 1630 - 1530 cm-1 for PC. The excellent coefficients of determination (R2), 0.9999 and 0.9998 were achieved for IBP and PC, respectively. The accuracy of calibration model was also verified through root mean square error of cross validation (RMSECV) which was found to be 0.064. This work clearly shows the capability of transmission FTIR spectroscopy for assessment of exact quantity of API to control the quality of finished products as well as during processing in pharmaceutical industries without involvement of any solvent.
Cite this paper: M. Mallah, S. Sherazi, S. Mahesar and A. Khaskheli, "Simultaneous Quantification of Ibuprofen and Paracetamol in Tablet Formulations Using Transmission Fourier Transform Infrared Spectroscopy," American Journal of Analytical Chemistry, Vol. 3 No. 8, 2012, pp. 503-511. doi: 10.4236/ajac.2012.38067.

[1]   D. R. Mehlisch, M. D. S. Aspley, S. E. Daniels and D. P. Bandy, “Comparison of the Analgesic Efficacy of Concurrent Ibuprofen and Paracetamol with Ibuprofen or Paracetamol alone in the Management of Moderate to Severe Acute Postoperative Dental Pain in Adolescents and Adults: A Randomized, Double-Blind, Placebo-Controlled, Parallel-Group, Single-Dose, Two-Center, Modified Factorial Study,” Clinical Therapeutics, Vol. 32, No. 5, 2010, pp. 882-895. doi:10.1016/j.clinthera.2010.04.022

[2]   D. Velasco, C. B. Danoux, J. A. Redondo, C. Elvira, J. S. Román, P. S. Wray and S. G. Kazarian, “pH-Sensitive Polymer Hydrogels Derived from Morpholine to Prevent the Crystallization of Ibuprofen,” Journal of Controlled Release, Vol. 149, No. 2, 2011, pp. 140-145. doi:10.1016/j.jconrel.2010.10.015

[3]   Y. Sun, K. Takaba, H. Kido, M. N. Nakashima and K. Nakashima, “Simultaneous Determination of Arylpropionic Acid Nonsteroidal Anti-Inflamatory Drugs in Pharmaceutical Formulations and Human Plasma by HPLC with UV Detection,” Journal of Pharmaceutical and Biomedical Analysis, Vol. 30, No. 5, 2003, pp. 1611-1619. doi:10.1016/S0731-7085(02)00549-6

[4]   S. Husain, M. Ki-fayatullah and R. Sekhar, “Simultaneous Determination of Ibu-profen and Acetaminophen in Pharmaceutical Preparations by Proton Magnetic Resonance Spectroscopy,” Journal of AOAC International, Vol. 77, No. 6, 1994, pp. 1443-1446.

[5]   H. E. Abdellatef, M. M. Ayad, S. M. Soliman and N. F. Youssef, “Spectrophotometric and Spec-trodensitometric Determination of Paracetamol and Drotaverine HCl in Combination,” Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Vol. 66, No. 4-5, 2007, pp. 1147-1151. doi:10.1016/j.saa.2006.05.028

[6]   P. Valderrama and R. J. Poppi, “Second Order Standard Addition Method and Fluores-cence Spectroscopy in the Quantification of Ibuprofen Enanti-omers in Biological Fluids,” Chemometrics and Intelligent Laboratory Systems, Vol. 106, No. 2, 2011, pp. 160-165. doi:10.1016/j.chemolab.2010.05.012

[7]   C. D. Palma, R. D. Paola, C. Perrotta, E. Mazzon, D. Cattaneo, E. Trabucchi, S. Cuzzocrea and E. Clementi, “Ibuprofen-Arginine Generates Nitric Oxide and Has En- hanced Anti-Inflammatory Effects,” Pharmacological Research, Vol. 60, No. 4, 2009, pp. 221-228. doi:10.1016/j.phrs.2009.06.002

[8]   A. O. Santini, J. E. D. Oliveira, H. R. Pezza and L. Pezza, “A New Potentiometric Ibuprofenate Ion Sensor Immobilized in a Graphite Matrix for Determination of Ibuprofen in Tablets,” Microchemical Journal, Vol. 84, No. 1-2, 2006, pp. 44-49. doi:10.1016/j.microc.2006.04.007

[9]   K. G. Kumar and R. Letha, “Determination of Paracetamol in Pure Form and in Dosage Forms Using N,N-Di-bromo Dimethylhydantoin,” Journal of Pharmaceutical and Biomedical Analysis, Vol. 15, No. 11, 1997, pp. 1725-1728. doi:10.1016/S0731-7085(96)01976-0

[10]   M. R. Khoshayand, H. Abdollahi, M. Shariatpanahi, A. Saadatfard and A. Mohammadi, “Simultaneous Spectrophotometric Determination of Paracetamol, Ibuprofen and Caffeine in Pharmaceuticals by Chemometric Methods,” Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Vol. 70, No. 3, 2008, pp. 491-499. doi:10.1016/j.saa.2007.07.033

[11]   Sirajuddin, A. R. Khaskheli, A. Shah, M. I. Bhanger, A. Niaz and S. A. Mahesar, “Simpler Spectrophotometric Assay of Paracetamol in Tablets and Urine Samples,” Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Vol. 68, No. 3, 2007, pp. 747-751. doi:10.1016/j.saa.2006.12.055

[12]   D. Basu, K. K. Mahalana-bis and B. Roy, “Application of Least Squares Method in Matrix Form: Simultaneous Determination of Ibuprofen and Paracetamol in Tablets,” Journal of Pharmaceutical and Bio-medical Analysis, Vol. 16, No. 5, 1998, pp. 809-812. doi:10.1016/S0731-7085(97)00111-8

[13]   Y. M. Issa, S. I. M. Zayed and I. H. I. Habib, “Simultaneous Determination of Ibu-profen and Paracetamol Using Derivatives of the Ratio Spectra Method,” Arabian Journal of Chemistry, Vol. 4, No. 3, 2011, pp. 259-263. doi:10.1016/j.arabjc.2010.06.044

[14]   E. Din?, C. Yücesoy and F. Onur, “Simultaneous Spectrophotometric Determination of Mefenamic Acid and Paracetamol in a Pharmaceutical Preparation Using Ratio Spectra Derivative Spectrophotometry and Chemometric Methods,” Journal of Pharmaceutical and Biomedical Analysis, Vol. 28, No. 6, 2002, pp. 1091-1100. doi:10.1016/S0731-7085(02)00031-6

[15]   T. Madrakian, A. Afkhami and M. Mohammadnejad, “Second-Order Advantage Applied to Simultaneous Spectrofluorimetric Determination of Paracetamol and Mefenamic Acid in Urine Samples,” Analytica Chimica Acta, Vol. 645, No. 1-2, 2009, pp. 25-29. doi:10.1016/j.aca.2009.05.002

[16]   M. A. Oliva, R. A. Olsina and A. N. Masi, “Selective Spectrofluorimetric Method for Paracetamol Determination through Coumarinic Compound Formation,” Talanta, Vol. 66, No. 1, 2005, pp. 229-235. doi:10.1016/j.talanta.2004.11.015

[17]   S. Azhagvuel and R. Sekar, “Method Development and Validation for the Simultaneous Determination of Cetirizine Dihydrochloride, Paracetamol, and Phenylpropanolamine Hydrochloride in Tablets by Capillary Zone Electrophoresis,” Journal of Pharmaceutical and Biomedical Analysis, Vol. 43, No. 3, 2007, pp. 873-878. doi:10.1016/j.jpba.2006.08.028

[18]   M. Kazutaka, Y. Takahisa, M. Takayoshi, T. Daisuke, S. Toshiaki, I. Yoshinori and O. Ryozo, “A Rapid and Simultaneous Determination of Several Analgesic Antiinflammatory Agents by Capillary Zone Elec-trophoresis,” Therapeutic Drug Monitoring, Vol. 25, No. 5, 2003, pp. 574-580. doi:10.1097/00007691-200310000-00006

[19]   B. C. Lourencao, R. A. Medeiros, R. C. R. Filho, L. H. Mazo and O. F. Filho, “Simultaneous Voltammetric Determination of Paracetamol and Caffeine in Pharmaceutical Formulations Using a Boron-Doped Diamond Electrode,” Talanta, Vol. 78, No. 3, 2009, pp. 748-752. doi:10.1016/j.talanta.2008.12.040

[20]   S. S. Nair, S. A. John and T. Sagara, “Simultaneous Determination of Paracetamol and Ascorbic Acid Using Tetraoctylammonium Bromide Capped Gold Nanoparticles Immobilized on 1,6-Hexanedithiol Modified Au Electrode,” Electrochimica Acta, Vol. 54, No. 27, 2009, pp. 6837-6843. doi:10.1016/j.electacta.2009.06.077

[21]   I. Baranowska, P. Markowski and J. Baranowski, “Simultaneous Determination of 11 Drugs Belonging to Four Different Groups in Human Urine Samples by Reversed-Phase High-Performance Liquid Chromatography Method,” Analytica Chimica Acta, Vol. 570, No. 1, 2006, pp. 46-58. doi:10.1016/j.aca.2006.04.002

[22]   K. R. I.-Lorenzini, J. A. Desmeules, M. Besson, J. L. Veuthey, P. Dayer and Y. Daali, “Two-Dimensional Liquid ChromatographyIon Trap Mass Spectrometry for the Simultaneous Determination of Ketorolac Enantiomers and Paracetamol in Human Plasma: Application to a Pharmacokinetic Study,” Journal of Chromatography A, Vol. 1216, No. 18, 2009, pp. 3851-3856. doi:10.1016/j.chroma.2009.02.071

[23]   X. Chen, J. Huang, Z. Kong and D. Zhong, “Sensitive Liquid Chromatogra-phy-Tandem Mass Spectrometry Method for the Simultaneous Determination of Paracetamol and Guaifenesin in Human Plasma” Journal of Chromatography B, Vol. 817, No. 2, 2005, pp. 263-269. doi:10.1016/j.jchromb.2004.12.011

[24]   H. G. Lou, H. Yuan, Z. R. Ruan and B. Jiang, “Simultaneous Determination of Paracetamol, Pseudoephedrine, Dextrophan and Chlor-pheniramine in Human Plasma by Liquid Chromatogra-phy-Tandem Mass Spectrometry,” Journal of Chromatography B, Vol. 878, No. 7-8, 2010, pp. 682-688.

[25]   M. H. Talpur, S. T. H. Sherazi and S. A. Mahesar, “Impact of Frying on Key Fatty Acid Ratios of Canola Oil,” European Journal of Lipid Science and Technology, Vol. 114, No. 2, 2012, pp. 222-228. doi:10.1002/ejlt.201100156

[26]   M. H. Khaskheli, S. T. H. Sherazi, H. M. Ujan and S. A. Mahesar, “Transmission FT-IR Spectroscopic Analysis of Human Kidney Stones,” Turkish Journal of Chemistry, Vol. 36, No. 3, 2012, pp. 477-483.

[27]   S. A. Mahesar, S. T. H. Sherazi, A. A. Kandhro, M. I. Bhanger, A. R. Khaskheli and M. Y. Talpur, “Evaluation of Important Fatty Acid Ratios in Poultry Feed Lipids by ATR FTIR Spectroscopy,” Vibrational Spectroscopy, Vol. 57, No. 2, 2011, pp. 177-181. doi:10.1016/j.vibspec.2011.06.009

[28]   S. T. H. Sherazi, M. Ali and S. A. Mahesar, “Application of Fourier-Transform Infrared (FT-IR) Transmission Spe- ctroscopy for the Estimation of Roxithromycin in Pharmaceutical Formulation,” Vibrational Spectroscopy, Vol. 55, No. 1, 2011, pp. 115-118. doi:10.1016/j.vibspec.2010.09.010

[29]   S. A. Mahesar, A. Bendini, L. Cerretani, M. B-Car- boginin and S. T. H. Sherazi, “Application of a Spectroscopic Method to Estimate the Olive Oil Oxidative Status,” European Journal of Lipid Science and Technology, Vol. 112, No. 12, 2010, pp. 1356-1362. doi:10.1002/ejlt.201000388

[30]   S. A. Mahesar, A. A. Kandhro, L. Cerretani, A. Bendini, S. T. H. Sherazi and M. I. Bhanger. “Determination of Total Trans Fat Content in Pakistani Cereal Based Foods by SB-HATR FT-IR Spectroscopy Coupled with Partial Least Square Regression,” Food Chemistry, Vol. 123, No. 4, 2010, pp. 1289-1293. doi:10.1016/j.foodchem.2010.05.101

[31]   S. T. H. Sherazi, M. Y. Talpur, S. A. Mahesar, A. A. Kandhro and S. Arain, “Main Fatty Acids Classes in Vegetable Oils by SB-ATR-Fourier Transform Infrared (FTIR) Spectroscopy,” Talanta, Vol. 80, No. 2, 2009, pp. 600-606. doi:10.1016/j.talanta.2009.07.030

[32]   S. T. H. Sherazi, A. Kandhro, S. A. Mahesar, M. I. Bhanger, M. Y. Talpur and S. Arain, “Application of Transmission FT-IR Spectroscopy for the Trans Fat Determination in the Industrially Processed Edible Oils,” Food Chemistry, Vol. 114, No. 1, 2009, pp. 323-327. doi:10.1016/j.foodchem.2008.09.058

[33]   F. R. van de Voort, J. Sedman and S. T. H. Sherazi, “Correcting For Underlying Absorption Interferences in FT-IR Trans Analysis of Edible Oils Using 2d Correlation Tech- niques,” Journal of Agricultural and Food Chemistry, Vol. 56, No. 5, 2008, pp. 1532-1537. doi:10.1021/jf0725068

[34]   F. R. van de Voort, J. Sedman and S. T. H. Sherazi, “Improved Determination of Isolated Trans Isomers in edible oils by Fourier Transform Infrared Spectroscopy Using Spectral Reconstitution,” Journal of the Association of Official Analytical Chemists International, Vol. 90, No. 2, 2007, pp. 446-451.

[35]   S. Naz, S. T. H. Sherazi. F. N. Talpur, M. Y. Taplur and H. Kara, “Instantaneous Fortitude of Bioactive Constituents in Deodorizer Distillate by GC-MS,” Journal of American Chemists Society (JAOCS), 2012, Accepted for publication.

[36]   AOAC International, “AOAC Guidelines for Single Laboratory Validation of Chemical Methods for Dietary Supplements and Botanicals,” Association of Official Analytical Chemists, Arlington, 2002.