ABSTRACT In this study we analyzed blood samples collected from 400 high risk patients for the prevalence of an inflammatory viral disease hepatitis B virus (HBV) and hepatitis C virus (HCV) with the help of standard kit assay and Enzyme-linked immunosorbent assay (ELISA). All the samples were selected randomly from the various places of District Gujrat, Pakistan. All the selected cases were first divided into four groups according to the age and sex (Group 1, Male below age 35 years; Group 2, Male above age 35 years; Group 3, Female below age 35 years; Group 4, Female above age 35 years), each group was comprised of 100 individual patients and analyzed for different parameters for the presence of HBV and HCV in comparison with positive and negative controls. The prevalence of HBV and HCV was higher in groups 2 (22%) and 4 (39%) respectively. Assay profile revealed that the incidence of HCV was higher in female patients as compare to the male patients. The present study indicates that more than 60% of the cirrhosis and hepatocellular carcinoma in the Region is attributable to hepatitis B virus (HBV) or hepatitis C virus (HCV) infection.
Cite this paper
Irshad, M. , Raza, M. , Anwar, Z. , Ikram, N. , Afroz, A. and Iqbal, H. (2012) Prevalence of viral HBV and HCV among different group patients in Gujrat Pakistan. Advances in Bioscience and Biotechnology, 3, 423-436. doi: 10.4236/abb.2012.324060.
 Liu, B., Li, J., Han, Y., Liu, Y., Kong, L., Cao, Y. and Huang, Z. (2010) Dynamic analysis of lymphocyte subsets of peripheral blood in patients with acute self-limited hepatitis B. Health 2, 736-741.
 Noah, D.N., Njouom, R., Bonny, A., Pirsou., Meli, J. and Sida, M.B. (2011) HBs antigene prevalence in blood donors and the risk of transfusion of hepatitis B at the central hospital of yaounde, Cameroon. Open J. Gastroenterol. 1, 23-27.
 Badar, N., Farooq, U., Ali, S., Nisar, N., Abubakar, M. and Qureshi, J.A. (2012) A Molecular Approach for Genotyping of Hepatitis B Virus Using Restriction Pattern Analysis of S Amplicon in Pakistan. Open J. Med. Microbiol. 2, 16-23.
 Lavenchy, D. (2004) Hapetitis B virus epidemiology, disease burden, treatment, and current and emerging prevention and control measures. J. Viral Hepatitis. 11, 97-107.
 Sato, S., Fujiyama, S. and Tanaka, M. (1994) Confection of hepatitis C virus in patients with chronic hepatitis B infection. J. Hepatol. 21, 159-66.
 Adjei, A.A., Armah, H.B. and Narter-Olaga, E.G. (2006) Seroprevalence of Cytomegalovirus among some voluntary blood donors at the 37 Military Hospital, Accra, Ghana. Ghana Med. J. 40, 99-104.
 Wild, C.P. and Hall, A.J. (2000) Primary prevention of hepatocellular carcinoma in developing countries. Mutat Res. 462, 381-393.
 Saher, F., Rehman, K., Qureeshi, J.A., Irshad, M. and Iqbal, H.M.N. (2012) Investigation of an inflammatory viral disease HBV in cardiac patients through Polymerase Chain Reaction. Adv. Biosci. Biotechnol. (Accepted).
 Akhtar, S., Moatter, T., Azam, S.I., Rahbar, M.H. and Adil, S. (1998) Prevalence and risk factors for interfamilial transmission of hepatitis C virus in Karachi, Pakistan. Baillieres Clin. Haematol. 11, 147-49.
 Hamid, H., Iqbal, R., Khan, M.H., Iftikhar, B., Aziz, S., Burki, F.K., Sethi, J., Hassan, M., Nisar, Y.B. and Krishan, J. (2008) Prevalence of hepatitis C in beta thalassaemic major. Gomal. J. Med. Sci. 6, 2.
 Mohammed, A.J., Qudah, A.A., Shishi, K.F., Sarayreh, A.A. and Quraan, L.A. (2009) Hepatitis C virus (HCV) infection in hemodialysis patients in the south of Jordan. Renal data Arab World, 20, 488-492.
 Herve, P. (2000). Transfusion safety: emergent or hypothetical risks. Transfus. Clin. Biol. 7, 30-38.
 Khan, A.A., Parveen, N., Mahaboob, V.S., Rajendraprasad, A., Ravindraprakash, H.R., Venkateswarlu, J., Rao, P., Pande, G., Narusu, M.L., Khaja, M.N., Pramila, R., Habeeb, A. and Habibullah, C.M. (2008) Management of hyperbilirubinemia in biliary atresia by hepatic progenitor cell transplantation through hepatic artery: a case report. Transplant Proc. 40, 1153-1155.
 Leung, N. (2002) Transmission of hepatitis B: case selection and duration of therapy. J. Gastroenterol. Hepatol. 17, 409-414.
 Fleming. (2000) The origin and evolution of hepatitis virus in humans. J. Gen. Virol. 82, 693-712.
 Rahman, M., Akhtar, G.N. and Lodi, Y. (2002) Transfusion transmitted HIV and HBV infection in Punjab, Pakistan. Pak. J. Med. Sci. 18, 18-25.