DNA fingerprinting of Bacillus cereus from diverse sources by restriction fragment length polymorphism analysis
ABSTRACT
Bacillus cereus is an opportunistic pathogen causing food poisoning manifested by diarrhoeal or emetic syndrome. It is closely related to animal and human pathogens Bacillus anthracis and the insect pathogen Bacillus thuringiensis. In the present study, antibiotic resistance, heavy metal tolerance & molecular typing of Bacillus cereus from diverse sources such as soil, sewage water, air, fresh water, sea water and milk were studied. Bacillus cereus resistant to Penicillin (10 units/ml) and Amoxycillin (10 µg/ml); sensitive to Cobalt and Mercury even at 1 mM concentration were determined. All the isolate had plasmid ranging in number from 3-5 and their molecular weight ranged from 3.0 kb-2.0 kb. Plasmid DNA band specific to 6,557 bp has been found in Bacillus cereus isolated from sewage water, air, fresh water, sea water and milk.
Bacillus cereus is an opportunistic pathogen causing food poisoning manifested by diarrhoeal or emetic syndrome. It is closely related to animal and human pathogens Bacillus anthracis and the insect pathogen Bacillus thuringiensis. In the present study, antibiotic resistance, heavy metal tolerance & molecular typing of Bacillus cereus from diverse sources such as soil, sewage water, air, fresh water, sea water and milk were studied. Bacillus cereus resistant to Penicillin (10 units/ml) and Amoxycillin (10 µg/ml); sensitive to Cobalt and Mercury even at 1 mM concentration were determined. All the isolate had plasmid ranging in number from 3-5 and their molecular weight ranged from 3.0 kb-2.0 kb. Plasmid DNA band specific to 6,557 bp has been found in Bacillus cereus isolated from sewage water, air, fresh water, sea water and milk.
Cite this paper
nullHemalatha, S. and Banu, N. (2010) DNA fingerprinting of Bacillus cereus from diverse sources by restriction fragment length polymorphism analysis. Advances in Bioscience and Biotechnology, 1, 136-144. doi: 10.4236/abb.2010.12019.
nullHemalatha, S. and Banu, N. (2010) DNA fingerprinting of Bacillus cereus from diverse sources by restriction fragment length polymorphism analysis. Advances in Bioscience and Biotechnology, 1, 136-144. doi: 10.4236/abb.2010.12019.
References
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[1] Kramer, J.M. and Gilbert, R.J. (1992) Bacillus cereus gastroenteritis. In: Tu, A.T. Ed., Food poisoining, Handbook of Natural toxins, 7, 119-153. [2] Helgason, E., Okstad, O.A., Caugant, D.A., Johansen, H.A., Fouet, A., Mock M. and Hegna, I. (2000) Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis – one species on the basis of genetic evidence. Applied and Environmental Microbiology, 66(6), 2627-2630. [3] Ivanova, N., Sorokin, A., Galleron, I.A., Candelon, B., Bhattacharya, A. K., Reznik, G., Mikhailova, N., Lapidus, A., Chu, L., Mazur, M., Goltsman, E., Walnus, T.L., Grechkin, G., Pusch, M., Haselkorn, M., Fonstein, S., Ehrlich, Y., Overbeek, R. and Kyrpides, N. (2003) Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis. Nature, 423, 87-91. [4] Drobniewski, F.A. (1998) Bacillus cereus and related species. Clinical Microbiology Reviews, 6(4), 324-328. [5] Field, D. and Wills, C. (1998) Abundant micro satellite polymorphism in Saccharomyces cerevisiae, and the different distributions of micro satellite in eight prokaryotes and S.cerevisiae, results from strong mutation pressures and a variety of selective forces. Proceedings of National Academy of Sciences, USA, 95(4), 1647-1652. [6] Lu, J., Knox, M.R., Ambrose, M.J., Brown J.K.M. and Ellis, T.H.N. (1996) Comparative analysis of genetic diversity in pea assessed by RFLP and PCR based methods. Theoritical and Applied Genetics, 93(7), 1103-1111. [7] Williams and Wilkins. (1994) Bergy’s Manual of Determinative Bacteriology. In: Williams and Wilkins, 9th Edition. [8] Sambrook, J. and David, W.R. (2001) Molecular cloning: A laboratory manual. Cold spring Harbor Laboratory Press, Newyork. [9] Forster, T.J. (1983) Plasmid mediated resistance to antimicrobial and toxic metal in bacteria. Microbiology Review, 47(3), 361-409. [10] Ghosh, A., Singh, A., Ramteke, P. and Singh, V. (2000) Characterisation of large plasmid encoding resistance to toxic heavy metals in Salmonella abortus equi. Biochemical and Biophysical Research Communications, 272(1), 6-11.