ABSTRACT A simple, sensitive and validated HPTLC method has been developed to determine Niacin and simvastatin simultaneously in synthetic mixture form. Chromatographic separation was achieved on a RP18 plate using a mixture of Methanol: Water: Acetic acid (60:40:0.1) at a wavelength of 237 nm. Linearity of themethod was found to be in the concentration range of
5000.0-25000.0 μg/ml for niacin and 100.0-500.0 μg/ml for simvastatin with correlation coefficient greater than 0.999. The method can be used for simultaneous determination of Niacin and Simvastatin.
Cite this paper
Tiwari, P. and Sathe, P. (2010) Development and validation of HPTLC method for niacin and simvastatin in binary combination. Advances in Bioscience and Biotechnology, 1, 131-135. doi: 10.4236/abb.2010.12018.
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