AJPS  Vol.3 No.7 , July 2012
Water Stress Effects on Leaf Growth and Chlorophyll Content but Not the Grain Yield in Traditional Rice (Oryza sativa Linn.) Genotypes of Assam, India II. Protein and Proline Status in Seedlings under PEG Induced Water Stress
Abstract: Abiotic stresses can directly or indirectly affect the physiological status of an organism by altering its metabolism, growth, and development. The leaf growth and Chlorophyll content has significantly shown to vary from the control ones while the grain yield was not affected. While many plant species naturally accumulate proline and protein as major organic osmolytes when subjected to different abiotic stresses. These compounds are thought to play adaptive roles in mediating osmotic adjustment and protecting sub cellular structures in stressed plants. Different approaches have been contemplated to increase the concentrations of proline like compounds in plants grown under stress conditions to increase their stress tolerance. Seven different traditional rice varieties of Assam were evaluated for their response to osmolyte production under physiological drought condition through simulation at three levels of osmotic stress of 0.15 bar, 0.25 bar and 0.56 bar of physiological drought initiated by polyethylene glycol (PEG 6000). Along with the evaluation for osmolyte response the different components of genotypic variation for six different drought-sustaining characters in the seven rice varieties were also substantiated. The results indicated that plant height and seed number have significant genotypic coefficient of variability (GCV) and heritability. Verities like Laodubi, Leserihali, Beriabhanga and Borah were screened out as the best drought sustaining variety.
Cite this paper: J. Chutia and S. Borah, "Water Stress Effects on Leaf Growth and Chlorophyll Content but Not the Grain Yield in Traditional Rice (Oryza sativa Linn.) Genotypes of Assam, India II. Protein and Proline Status in Seedlings under PEG Induced Water Stress," American Journal of Plant Sciences, Vol. 3 No. 7, 2012, pp. 971-980. doi: 10.4236/ajps.2012.37115.

[1]   R. K. Sarkar, J. N. Reddy, S. G. Sharma and M. I. Abdelbagi, “Physiological Basis of Submergence Tolerance in Rice and Implication for Crop Improvement,” Current Science, Vol. 9, No. 7, 2006, pp. 899-906.

[2]   J. K. Zhu, “Salt and Drought Stress Signal Transduction in Plants,” Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 53, 2002, pp. 247-273.

[3]   J. M. Cutler, K. W. Sahan and P. L. Steponkus, “Alteration of the Internal Water Relations of Rice in Response to Drought Hardening,” Crop Science, Vol. 20, No. 3, 1980, pp. 307-310. doi:10.2135/cropsci1980.0011183X002000030004x

[4]   Y. Jiang, S. E. Macdonald and J. J. Zwiazak, “Effects of Cold Storage and Water Stress on Water Relations and Gas Ex-change of White Spruce (Picea glauca) Seedlings,” Tree Physiology, Vol. 15, No. 4, 1995, pp. 267- 273.

[5]   A. Ran-jbarfordoei, R. Samson, P. V. Damne and R. Lemeur, “Effects of Drought Stress Induced by Polyethylene Glycol on Pigment Content and Photosynthetic Gas Exchange of Pistacia khinjuk and P mutica,” Photosynthetic, Vol. 38, No. 3, 2000, pp. 443-447. doi:10.1023/A:1010946209484

[6]   O. Chezen, W. hartwig and P. M. Newman, “The Different Effects of PEG-6000 and NaCl on Leaf Development Are Associated with Differential Inhibition of Root Water Transport,” Plant Cell, Vol. 18, No. 7, 1995, pp. 727-735. doi:10.1111/j.1365-3040.1995.tb00575.x

[7]   M. Ashraf and J. W. O’Leary, “Effect of Drought Stress on Growth, Water Rela-tions and Gas Exchange of Two Lines of Sunflower Differing in Degree of Salt Tolerance,” International Journal of Plant Sciences, Vol. 157, No. 6, 1996, pp. 729-732. doi:10.1086/297395

[8]   K. Singh and B. S. Afria, “Seed Technological Approach for Evaluation of Drought Tolerance in Wheat Germplasm,” In: T. P. Yadav and C. Ram, Eds., Proc. Nation Semin. Seed Sci Tech. HAU, Hissar. 1988, pp. 72-178.

[9]   K. Singh and B. L. Kakralya, “Seed Physiological Approach for Evaluation of Drought Tolerance in Groundnut Stress and Environmental Plant Physiology,” (Eds. K K Bora and Karan Singh and Arvind Kumar pp Pointer Publishers, Jaipur, Rajasthan, 2001, pp. 45-152,

[10]   M. El. Midaoui, H. Serieys and F. Kaan, “Effects of Osmotic and Water Stresses on Root and Shoot Morphology and Seed Yield in Sunflower (Helianthus annuus L.) Genotypes Breed for Morocco or Issued from Introgression with H. argophyllus T. & G. and H. debilis Nutt,” HELIA, Vol. 26, Nr. 38, 2003, pp. 1-16

[11]   J. Zhang and M. B. Kirkham, “Water Relations of Water Stressed Split Root C4 and C3 Plants,” American Journal of Botany, Vol. 82, No. 10, 1995, pp. 1220-1229. doi:10.2307/2446244

[12]   N. Holmberg and L. Bulow, “Im-proving Stress Tolerance in Plant by Gene Transfer,” Trends in Plant Science, Vol. 3, No. 2, 1998, pp. 61-66. doi:10.1016/S1360-1385(97)01163-1

[13]   M. Kasuga, W. Liu, S. Miura, K. Yamaguchi-Shinozaki and K. Shinozaki, “Im-proving Plant Drought, Salt, and Freezing Tolerance by Gene Transfer of a Single Stress- Inducible Transcription Factor,” Nature Biotechnology, Vol. 17, 1999, pp. 287-291. doi:10.1038/7036

[14]   R. Serrano, F. A. Culianz-Macia and V. Moreno, “Genetic Engineering of Salt and Drought Tolerance with Yeast Regulatory Genes,” Scientia Horticulturae, Vol. 78, No. 1-4, 1999, pp. 261-269. doi:10.1016/S0304-4238(98)00196-4

[15]   P. M. Hasegawa, R. A. Bressan, J. K. Zhu and H. J. Bohnert, “Plant Cellular and Molecular Responses to High Salinity,” Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 51, 2000, pp. 463-499. doi:10.1146/annurev.arplant.51.1.463

[16]   J. K. Zhu, “Plant Salt Tolerance,” Trends in Plant Science, Vol. 6, No. 2, 2001, pp. 66-71. doi:10.1016/S1360-1385(00)01838-0

[17]   V. Prabhavathi, J. S. Yadav, P. A. Kumar and M. V. Rajam, “Abiotic Stress Toler-ance in Transgenic Eggplant (Solanum melongena L.) by In-troduction of Bactrial Mannitol Phophodehydrogenase Gene,” Molecular Breeding, Vol. 9, No. 2, 2002, pp. 137-147. doi:10.1023/A:1026765026493

[18]   D. Rontein, G. Basset and A. D. Hanson, “Metabolic Engineering of Osmoprotectant Ac-cumulation in Plants,” Metabolic Engineering, Vol. 4, No. 1, 2002, pp. 49-56. doi:10.1006/mben.2001.0208

[19]   D. P. Singh, “Water deficit stress in Stress physiology,” New age Publishers, New Delhi, 2003, pp. 64-79.

[20]   M. Ashraf and M. R. Foolad, “Role of Glycine Betaine and Proline in Improving Plant Abiotic Stress Resistance,” Environmental and Experimental Botany, Vol. 59, No. 2, 2007, pp. 206-216. doi:10.1016/j.envexpbot.2005.12.006

[21]   B. N. Jha and R. A. Singh, “Physiological Responses of Rice Varieties to Different Levels of Moisture Stress,” Plant Physiology, Vol. 2, No. 1, 1997, pp. 81-84.

[22]   Association of Official Seed Analysis (AOSA), “Seed Vigor Testing Handbook,” Contribution No. 32 to the handbook on Seed Testing, 1983.

[23]   L. S. Bates, R. P. Walden and I. D. Teare, “Rapid Determination of Free Proline for Water-Stress Studies,” Plant and Soil, Vol. 39, No. 1, 1973, pp. 205-207. doi:10.1007/BF00018060

[24]   E. P. Chinard, The Journal of Biological Chemistry, Vol. 199, 1952, p. 91.

[25]   O. H. Lowry, N. J. Rosenbrough, A. L. Farr and R. J. Randall, “Protein Measurement with Folin Phenol Reagent,” The Journal of Bio-logical Chemistry, Vol. 193, No. 1, 1951, pp. 265-275.

[26]   G. W. Burton, “Quantitative Inheritance in Grasses,” Proceedings of 6th Intern, Grasslands Congress, Vol. 1, 1952, pp. 277-283.

[27]   H. W. Johnson, C. L. Haward and A. R. Khan, “Genotypic, Phenotypic Correlations in Soyabean and Their Implication in Selection,” Agronomy Journal, Vol. 47, No. 10, 1955, pp. 477-483. doi:10.2134/agronj1955.00021962004700100008x

[28]   H. W. Johnson, H. F. Robinson and R. E. Comstock, “Estimates of Genetic and Environmental Variability in Soyabean,” Agron-omy Journal, Vol. 47, No. 7, 1955, pp. 314-318. doi:10.2134/agronj1955.00021962004700070009x

[29]   N. C. Turner, “Role of Shoot Characteristics in Drought Resistance of Crop Plants,” In: Drought Resistance in Crops with Emphasis on Rice, IRRI, 1982, pp. 115-134

[30]   T. T. Chang, G. C. Loresto and O. Tagumpay, “Screening Rice Germplasm for Drought Resistance,” Sabard Journal, Vol. 6, 1974, pp. 9-16.

[31]   Sharif-zadeh and J. Mohsen, “Influence of Priming Tech-niques on Seed Germination Behavior of Maize Inbreed Lines (Zea mays L.),” Journal of Agricultural and Biological Science, Vol. 3, No. 3, 2008, pp. 22-25

[32]   C. B. Shah and R. S. Loomis, “Ribonucleic Acid and Protein Metabolism in Sugar Beet during Drought,” Plant Physiology, Vol. 18, 1975, pp. 240-254.

[33]   A. Hadas, “Water Uptake and Germination of Leguminous Seeds under Changing External Water Potential in Osmoticum Solution,” Journal of Experimental Botany, Vol. 27, No. 3, 1976, pp. 480-489. doi:10.1093/jxb/27.3.480

[34]   J. H. Everiff, “Seed Germina-tion Characteristics of Three Weedy Plants Species from South Taxas,” Journal of Range Management, Vol. 36, No. 2, 1983, pp. 246-249. doi:10.2307/3898175

[35]   M. G. Huck, B. Kleper and M. M. Taylor, “Diurnal Variation in Root Diameter,” Plant Physiol-ogy, Vol. 45, No. 4, 1970, pp. 529-530. doi:10.1104/pp.45.4.529

[36]   K. K. Baruahand, K. Singh and A. Kumar, “Evaluation of Drought Tolerance in Groundnut Stress and Environmental Plant Physiology,” Pointer Publishers, Jaipur, Rajasthan, 1998, pp. 145-152.

[37]   R. Serraj and T. R. Sinclair, “Osmolyte Accumulation: Can It Really Help Increase Crop Yield under Drought Conditions?” Plant, Cell & Envi-ronment, Vol. 25, No. 2, 2002, pp. 333-341. doi:10.1046/j.1365-3040.2002.00754.x

[38]   P. A. Genkel, N. A. Satarova and E. K. Tvorus, “Effect of Drought on Protein Synthesis and the State of Ribosomed in Plants,” Fiziol, Rast., Vol. 14, 1967, pp. 898-907.

[39]   T. C. Hsiao, “Rapid Changes in the Levels of Polyribosomes in Zea mays in Reponse to Wa-ter Stress,” Plant Physiology, Vol. 46, No. 2, 1970, pp. 281-285. doi:10.1104/pp.46.2.281

[40]   J. J. Chinoy, Y. D. Singh and K. Gurumurti, “Biosynthesis of Ascorbic Acid and Mobilization Patterns of Macromolecules during Water Stressing Germinat-ing Ciser Seedling,” Biology Plant (Prague), Vol. 16, 1974, pp. 301-307.

[41]   A. Tyagi, N. Kumar and R. K. Sairam, “Efficacy of RWC, Membrane Stability, Osmotic Potential, Endogenous ABA and Root Biomass as Indices for Selection against Water Stress in Rice,” Indian Journal of Plant Physiology, Vol. 4, No. 4, 1999, pp. 302-306.

[42]   K. K. Baruah, S. S. Bhuyan, T. J. Ghosh and A. K. Pathak, “Response of Rice Genotypes to Moisture Stress Imposed at Seedling Stage,” Indian Journal of Plant Physiology, Vol. 3, No. 3, 1998, pp. 181-184.

[43]   A. H. Bunting and A. H. Kasam, “Principles of Crop Water Use, Dry Matter Production That Govern Choice of Crops and Systems,” In: F. R. Bidinger and C. Johansen, Eds., Drought Research Priorities for the Dryland Tropics, ICRISAT, Pathancheru, 1988, pp. 43-61.

[44]   M. R. Foolad, “Comparison of Salt Tol-erance during Seed Germination and Vegetative Growth in Tomato by QTL Mapping,” Genome, Vol. 42, No. 4, 1999, pp. 727- 734. doi:10.1139/g98-163

[45]   M. R. Foolad, P. Subbiah, C. Kramer, G. Hargrave and G. Y. Lin, “Genetic Relationships among Cold, Salt and Drought Tolerance during Seed Germi-nation in an Interspecific cross of Tomato,” Euphytica, Vol. 130, No. 2, 2003, pp. 199-206. doi:10.1023/A:1022871500583

[46]   M. R. Foolad, L. Zhang and P. Subbiah, “Genetics of Drought Tolerance during Seed Germination in Tomato: Inheritance and QTL Mapping,” Ge-nome, Vol. 46, No. 4, 2003, pp. 536-545. doi:10.1139/g03-035

[47]   M. Ashraf and P. J. C. Harrish, “Potential Biochemical Indicators of Salinity Tolerance in Plants,” Plant Science, Vol. 166, No. 1, 2004, pp. 3-16. doi:10.1016/j.plantsci.2003.10.024