ABSTRACT In this study, Artemisia biennis was seeded in a greenhouse and raised to an average plant height of 100 cm. Aboveground plant portions were harvested and partitioned into leaves and stems, and dried; while roots were either removed from some soil (soil – roots) or left in soil (soil + roots). Greenhouse studies were conducted to evaluate the allelopathic potential of A. biennis leaves, roots, and stems; and soil – roots, and soil + roots on Solanum melanocerasum plant height and fresh weight plant–1. When 5 g of root and stem biomass were added to soil, S. melanocerasum plant height and fresh weight plant–1 was reduced by 75 and 88%, respectively. In contrast, 5 g of leaf biomass caused an increase in S. melanocerasum plant height and fresh weight plant–1 by 35% and 43%, respectively; whereas, 20 g of leaf biomass depressed both variables by 50% and 65%, also respectively. Plant height was more suppressed when S. melanocerasum grew in soil – roots as opposed to soil + roots, whereas fresh weight plant–1 was similar between soil treatments. S. melanocerasum plant height was reduced by 70 and 55% when grown in soil – roots and soil + roots, respectively. In contrast, S. melanocerasum fresh weight plant–1 was reduced by 76% in both soil treatments. The reduction in S. melanocerasum plant attributes in this study is indicative of the allelopathic potential of A. biennis. Furthermore, A. biennis allelopathy is differenttially expressed among plant parts, primarily in roots. This may explain how A. biennis is capable of dominating a habitat once it becomes established. The presence of extractable compounds with herbicidal activity could increase the potential usefulness of A. biennis.
Cite this paper
Kegode, G. , Ciernia, M. and Vlieger, D. (2012) Allelopathic potential of Artemisia biennis (biennial wormwood). Agricultural Sciences, 3, 582-587. doi: 10.4236/as.2012.34070.
 Hall, H.M. and Clements, F.E. (1923) The phylogenetic method in Taxonomy. The North American species of Artemisia, Chrysothamanus, and Atriplex. Carnegie Institution of Washington, Washington DC.
 Jehlik, V. (1984) Artemisia biennis in Czechoslovakia. Prelsalia. Academia, Praha.
 Kegode, G.O. and Christoffers, M.J. (2003) Intriguing world of weeds: Biennial wormwood (Artemisia biennis Willd.). Weed Technology, 17, 646-649.
 Stevens, O.A. (1932) The number and weight of seeds produced by weeds. American Journal of Botany, 19, 784- 794. doi:10.2307/2436042
 Mahoney, K.J. and Kegode, G.O. (2004) Biennial wormwood (Artemisia biennis) biomass allocation and seed production. Weed Science, 52, 246-254.
 Duke, S.O., Vaughn, K.C., Croom, E.M. Jr. and Elsohly, H.N. (1987) Artemi-sinin, a constituent of annual worm- wood (Artemisia annua), is a selective phytotoxin. Weed Science, 35, 499-505.
 Ferreira, J.F.S., Simon, J.E. and Janick, J. (1997) Artemisia annua: Botany, horticulture, pharmacology. Horticultural Reviews, 19, 319-371.
 Kawamoto, H., Sekine, H. and Furaya, T. (1999) Production of artemi-sinin and related sesquiterpenes in Japanese Artemisia annua during a vegetation period. Planta Medica, 65, 88-89. doi:10.1055/s-2006-960449
 Kim, J.H., (1996) Seasonal variation in concentration and composition of monoterpenes from Artemisia princeps var. orientalis. Korean Journal of Ecology, 19, 321-328.
 Barney, J.N., Hay, A.G. and Weston, L.A. (2005) Isolation and characterization of allelopathic volatiles from mug- wort (Artemisia vulgaris). Journal of Chemical Ecology, 31, 247-265. doi:10.1007/s10886-005-1339-8
 Johnson, W.G., Hartzler, R.G. and Nordby, D.E. (2004) Weeds to watch: Weeds that seem to be expanding their habitat range. Abstr. North Central Weed Sci. Soc, 59, 133.
 Ciernia, M.G. and Kegode, G.O., (2003) Allelopathic potential of biennial wormwood. North Central Weed Science Society, 58, 69.
 Kegode, G.O. and Ciernia, M.G. (2005) Biennial wormwood allelopathic potential. Weed Science Society of America, 45, 187.
 Chung, I.M., Kim, K.H., Ahn, J.K., Lee, S.B., Kim, S.H. and Hahn, S.J. (2003) Comparison of allelopathic potential of rice leaves, straw, and hull extracts on barnyardgrass. Agronomy Journal, 95, 1063-1070.
 Lydon, J., Teasdale, J.R. and Chen, P.K. (1997) Allelo- pathic activity of annual wormwood (Artemisia annua) and the role of artemisinin. Weed Science, 45, 807-811.
 Kim, J.H. (1997) Variation of monterpenoids in Artemisia feddei and Artemisia scoparia. Journal of Plant Biology, 40, 26-274. doi:10.1007/BF03030459
 Yun, K.W. and Choi, S. (2003) Seasonal variation in al- lelopathic potential of Artemisia princeps var. orientalis on plants and microbes. Journal of Plant Biology, 46, 105-110. doi:10.1007/BF03030438
 Van Geldre, E., Vergauwe, A. and Van den Eeckhout, E. (1997) State of the art of the production of the antimalarial compound artemisinin in plants. Plant Molecular Biology, 33, 199-209. doi:10.1023/A:1005716600612
 Lee, D.L., Prisbylla, M.P., Cromartie, T.H., Dagarin, D.P., Howard, S.E., Pro-van, W.M., Ellis, M.K., Fraser, T. and Mutter, L.C. (1997) The discovery and structural requirements of inhibitors of p-hydroxyphenylpyruvate dioxybenase. Weed Science, 45, 95-102.
 Weston, L.A. (1996) Utilization of allelopathy for weed management in agroecosystems. Agronomy Journal, 88, 860-866.
 Duke, S.O., Scheffler, B.E., Dayan, F E., Weston, L.A. and Ota, E. (2001) Strategies for using transgenes to pro- duce allelopathic crops. Weed Technology, 15, 826-834.
 Lopes-Lutz, D., Alviano, D.S., Alviano, C.S. and Kolod- ziejczyk, P.P. (2008) Screening of chemical composition, antimicrobial and antioxidant activities of Arte-misia essential oils. Phytochemistry, 69, 1732-1738.
 Inderjit and Dakshini, K.M.M. (1994) Allelopathic Effect of Pluchea lanceolata (Asteraceae) on characteristics of four soils and tomato and mustard growth. American Journal of Botany, 81, 799-804. doi:10.2307/2445760