ABSTRACT Four groups of antibiotic activity compounds were separated and purified from the ethyl acetate fraction of a broth culture of Streptomyces herbaricolor HNS2-2.Trace analyses were performed and anti-microbial activities were determined. The antibiotic activity compounds were identified as quercetin dehydrate ①, p-hydroxyphenyl ②, 4’-hydroxyflavanone ③, and 3-hydroxyflavone ④ based on spectroscopic data. Compounds 1-4 showed significant antimicrobial activities against Staphylococcus aureus and the tobacco mosaic virus in vitro, suggesting their potential agricultural and medical applications.
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