ABSTRACT An epithelial receptor for the Lactobacillus surface adhesion factor was isolated and purified from mucus of the gastrointestinal tract of SPF chickens, using chromatography. The purified protein was analyzed with discontinuous, native gel electrophoresis and binding assay with cultured intestinal epithelial cells. A single band was obtained after purification. The molecular weight of this band was about 60 KDa. The purified protein inhibited the attachment of Lactobacillus to intestinal epithelial cells, suggesting that it is the gastrointestinal receptor for the surface adhesion factor of Lactobacillus.
Cite this paper
Lin, X. , Wang, Z. , Niu, Z. and Peng, J. (2012) Purification and characterization of a gastrointestinal tract epithelial receptor for the adhesion factor of Lactobacillus. Advances in Bioscience and Biotechnology, 3, 274-277. doi: 10.4236/abb.2012.33038.
 Wang, J.Z. and Fan, M. (2002) Protein technology. Science Press, Beijing.
Zheng, Y.J., Pan, L.J. and Wang, L.S. (1999) Purify adhension from biobacillus. Chinese Journal of Mocrobioloy and Immunology, 19, 196.
 Zhao, B. and He, S.J. (2002) Microbiological experiment. Beijing Science Press, Beijing.
 Bernet, M.F., Brassart, D. and Neeser, J.R. (1994) Lacto-bacillus acidophilus LA 1 binds to Cultured intestinal cell Lines and inhibits cell attachment cell invasion by enterovirulen. Bacteria Gut, 35, 483-489.
 Henriksson, A. and Conway, P.L. (1996) Adhesion of Lactobacillus fermentum 104-S to porcine stomach mucus. Current Microbiology, 33, 31-34.
 Turner, M.S., Peter, T. and Louisem, H. (1997) Identification and characterization of a basic cell surface-located protein from Lacto-bacillus fermentum BR11. Journal of Bacteriology, 179, 3310-3316.
 Sansonetti, P.J. (1991) Molecular and cellular bases of Shigella flexneri virulence. Bulletin of the National Academy of Medicine, 175, 803-809.
 Shandong Agriculture University (2002) Biochemistry and molecular biological test technology. Shandong Publishing Company, Tai’an.
 Sellwood, R. (1980) The interaction of the K88 antigen with porcine intestinal epithelial cell brush borders. Biochimica et Biophysica Acta, 632, 326-335.
 Takahiro, T., Ritva, V. and Benita, W. (1995) A collagen-binding s-layer protein in Lactobacillus crispatus. Applied and Environmental Microbiology, 61, 2467-2471