AiM  Vol.2 No.2 , June 2012
Effect of Mild Sonication Conditions on the Attributes of Lactobacillus delbrueckii ssp. bulgaricus LB-12
ABSTRACT
Lactobacillus delbrueckii ssp. bulgaricus is a widely used bacterium for the production of some fermented dairy products. Mild sonication intensity condition is a non-destructive technique that uses sound waves to cause cavitation in aqueous solutions and may improve the permeability of membranes, speed up the transfer of substrates and promote cellular growth and propagation. The objective was to determine the effect of mild sonication intensities at different temperatures on growth, bile tolerance and protease activity of Lactobacillus delbrueckii ssp. bulgaricus LB-12. The treatments were four sonication intensities (8.07, 14.68, 19.83 and 23.55 W/cm2) randomized at three different temperatures (4℃, 22℃ and 40℃). The energy input (1500 J) was kept constant in all treatments. Control samples did not receive any sonication treatment. Growth and bile tolerance were determined every 2 h for 12 h of incubation. Protease activity was determined at 0, 12 and 24 h. Mild sonication conditions included 1) mild sonication intensities, 2) temperatures and 3) times, all three of which played a role in influencing the desirable attributes of Lactobacillus delbrueckii ssp. bulgaricus LB-12. Of all the mild sonication intensities studied, 14.68 W/cm2 had the best overall influence at certain time points forimproving the bile tolerance and growth at 4℃ and protease activity at 40℃. Mild sonication intensity of 23.55 W/cm2 had the best overall influence at certain time points for protease activity of at 22℃. Some mild sonication conditions could be recommended for improvement of some characteristics of Lactobacillus delbrueckii ssp. bulgaricus LB-12.

Cite this paper
M. Moncada, K. J. Aryana and C. Boeneke, "Effect of Mild Sonication Conditions on the Attributes of Lactobacillus delbrueckii ssp. bulgaricus LB-12," Advances in Microbiology, Vol. 2 No. 2, 2012, pp. 104-111. doi: 10.4236/aim.2012.22014.
References
[1]   Code of Federal Regulations, “Low Fat Yogurt,” 2010.

[2]   International Dairy Food Association, “Dairy Facts,” 2010. http://www.idfa.org/files/_willow/products/481_DF2010.pdf

[3]   GIA, Yogurt sales by Global Industry Analyst Inc, 2010. http://www.nutritionaloutlook.com/category/company/global-industry-analysts-inc

[4]   D. Granato, G. F. Branco, A. Gomes-Cruz, J. A. Fonseca-Farias and N. P. Shah, “Probiotic Dairy Products as Functional Foods,” Comprehensive Reviews in Food Science and Food Safety, Vol. 9, No. 5, 2010, pp. 455-470. doi:10.1111/j.1541-4337.2010.00120.x

[5]   D. J. McClement, “Advance in the Application of Ultrasound in Food Analysis and Processing,” Trends in Food Science and Technology, Vol. 6, No. 9, 1995, pp. 293-299. doi:10.1016/S0924-2244(00)89139-6

[6]   W. G. Pitt and A. Ross, “Ultrasound Increases the Rate of Bacterial Cell Growth,” Biotechnology Progress, Vol. 19, No. 3, 2003, pp. 1038-1044. doi:10.1021/bp0340685

[7]   N. P. Shah and P. Jelen, “Survival of Lactic Acid Bacteria and Their Lactases under Acidic Conditions,” Journal of Food Science, Vol. 55, No, 2, 1990, pp. 506-509. doi:10.1111/j.1365-2621.1990.tb06797.x

[8]   D. Wang, M. Sakakibara, N. Kondoh and K. Suzuki, “Ultrasound-Enhanced Lactose Hydrolysis in Milk Fermentation with Lactobacillus bulgaricus,” Department of Applied Chemistry and Biotechnology, Vol. 65, No. 1, 1996, pp. 86-92.

[9]   M. E. Kreft and P. Jelen, “Stability and Activity of β-Galactosidase in Sonicated Cultures of Lactobacillus delbrueckii ssp. bulgaricus 11842 as Affected by Temperature and Ionic Environments,” Journal of Food Science, Vol. 65, No. 8, 2000, pp. 1364-1368. doi:10.1111/j.1365-2621.2000.tb10613.x

[10]   D. Wang and M. Sakakibara, “Lactose Hydrolysis and β-Galactosidase Activity in Sonicated Fermentation with Lactobacillus Strains,” Ultrasonics Sonochemestry, Vol. 4, No. 3, 1997, pp. 255-261.

[11]   R. I. Dave and N. P. Shah, “Evaluation of Media for Selective Enumeration of Streptococcus thermophilus, Lactobacillus delbrueckii ssp. bulgaricus, Lactobacillus acidophilus, and Bifidobacteria,” Journal of Dairy Science, Vol. 79, No. 9, 1996, pp. 1529-1536. doi:10.3168/jds.S0022-0302(96)76513-X

[12]   Y. M. Lin and C. M. Young, “Folate Levels in Cultures of Lactic Acid Bacteria,” International Dairy Journal, Vol. 10, No. 5-6, 2000, pp. 409-413. doi:10.1016/S0958-6946(00)00056-X

[13]   C. P. Champagne, Y. Raymond, J. Gonthier and P. Audet. “Enumeration of the Contaminating Bacterial Microbiota in Unfermented Pasteurized Milks Enriched with Probiotic Bacteria,” Canadian Journal of Microbiology, Vol. 55, No. 4, 2009, pp. 410-418. doi:10.1139/W08-151

[14]   D. I. A. Pereira and G. R. Gibson, “Cholesterol Assimilation by Lactic Acid Bacteria and Bifidobacteria Isolated from the Human Gut,” Applied of Environmental Microbiology, Vol. 68, No. 9, 2002, pp. 4689-4693. doi:10.1128/AEM.68.9.4689-4693.2002

[15]   C. J. Oberg, B. C. Weimer, L. V. Moyes, R. J. Brown and G. H. Richardson, “Proteolytic Characterization of Lactobacillus delbrueckii ssp. bulgaricus strains by the o-Phthaldialdehyde Test and Amino Acid Analysis,” Journal of Dairy Science, Vol. 74, No. 2, 1991, pp. 398-403. doi:10.3168/jds.S0022-0302(91)78181-2

[16]   M. T. Liong and N. P. Shah, “Acid and Bile Tolerance and Cholesterol Removal Ability of Lactobacilli Strains,” Journal of Dairy Science, Vol. 88, No. 1, 2005, pp. 55-66. doi:10.3168/jds.S0022-0302(05)72662-X

[17]   E. Simova, Z. Simov, D. Beshkova, G. Frengova, Z. Dimitrov and Z. Spasov, “Amino Acid Profiles of Lactic Acid Bacteria, Isolated from Kefir Grains and Kefir Starter Made from Them,” International Journal of Food Microbiology, Vol. 107, No. 2, 2006, pp. 112-123. doi:10.1016/j.ijfoodmicro.2005.08.020

[18]   Y. Kobayashi, D. Sakai, T. Iwashina, S. Iwabuchi and J. Mochida, “Low-Intensity Pulsed Ultrasound Stimulates Cell Proliferation, Proteoglycan Synthesis and Expression of Growth Factor-Related Genes in Human Nucleus Pulposus Cell Line,” European Cells Materials, Vol. 17, 2009, pp. 15-22.

[19]   P. A. Clark and J. H. Martin, “Selection of Bifidobacteria for Use as Dietary Adjuncts in Cultured Dairy Foods: III. Tolerance to Simulated Bile Concentrations of Human Small Intestines,” Cultured Dairy Products Journal, Vol. 29, No. 3, 1994, pp. 18-21.

[20]   S. Lick, K. Drescher and K. J. Heller, “Survival of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus in the Terminal Ileum of Fistulated G?t-tingenminipigs,” Applied of Environmental Microbiology, Vol. 67, No. 9, 2001, pp. 4137-4143. doi:10.1128/AEM.67.9.4137-4143.2001

 
 
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