ABSTRACT Aeromonas hydrophila isolated from fish (Labeo rohita), pond water, river water, raw meat of chicken and mutton and raw cow milk were characterized through Random Amplified Polymorphic DNA (RAPD) analysis and Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS PAGE) of cellular proteins. RAPD-PCR amplification of bacterial DNA was done by using ten random primers (OPA-01 to 10) and found some distinct banding pattern on agarose gel. RAPD profile was studied with each isolate and absolute polymorphism indicating its application as an ideal tool for molecular characterization. Other methods like morphological, serological and biochemical characterization gives contradictory results and total cellular protein profiling does not show any significant polymorphism for identification and discrimination.
Cite this paper
A. Sarkar, M. Saha, A. Patra and P. Roy, "Characterization of Aeromonas hydrophila through RAPD-PCR and SDS-PAGE Analysis," Open Journal of Medical Microbiology, Vol. 2 No. 2, 2012, pp. 37-40. doi: 10.4236/ojmm.2012.22005.
 M. Altwegg, “Subtyping Methods for Aeromonas Species,” In: B. Austin, M. Altwegg, P. J. Gosling and S. W. Joseph, Eds., The Genus Aeromonas, John Wiley & Sons, Chichester, 1996, pp. 109-126.
 M. Altwegg, “Aeromonas and Plesiomonas,” In: P. Murray, E. Baron, M. Pfaller, F. Tenover and R. Yolken, Eds., Manual of Clinical Microbiology, ASM Press, Washington DC, 1999, pp. 507-516.
 G. Brandi, M. Sisti, G. F. Schiavano, L. Salvaggio and A. Albano, “Survival of Aeromonas hydrophila, Aeromonas caviae and Aeromonas sobria in Soil,” Journal of Applied Microbiology, Vol. 81, No. 4, 1996, pp. 439-444.
 M. Kupfer, P. Kuhnert, B. M. Korczak, R. Peduzzi and A. Demarta, “Genetic Relationships of Aeromonas Strains Inferred from 16S rRNA, gyrB and rpoB Gene Sequences,” International Journal of Systematic and Evolutionary Microbiology, Vol. 56, No. 12, 2006, pp. 2743- 2751. doi:10.1099/ijs.0.63650-0
 M. Altwegg, H. K. Geiss and B. J. Freij, “Aeromonas as a Human Pathogen,” Critical Reviews in Microbiology, Vol. 16, No. 4, 1989, pp. 253-286.
 R. D. Isaacs, S. D. Paviour, D. E. Bunker and S. D. R. Lang, “Wound Infection with Aerogenic Aeromonas Strains: A Review of Twenty-Seven Cases,” European Journal of Clinical Microbiology & Infections Diseases, Vol. 7, No. 3, 1988, pp. 355-360. doi:10.1007/BF01962336
 J. M. Janda, “Recent Advances in the Study of the Taxonomy, Pathogenicity, and Infectious Syndromes Associated with the Genus Aeromonas,” Clinical Microbiology Reviews, Vol. 4, No. 4, 1991, pp. 397-410.
 E. J. Kuijper, L. van Alphen, E. Leenders and H. C. Zanen, “Typing of Aeromonas Strains by DNA Restriction Endonuclease Analysis and Polyacrylamide Gel Electrophoresis of Cell Envelopes,” Journal of Clinical Microbiology, Vol. 27, No. 6, 1989, pp. 1280-1285.
 N. P. Moyer, G. Martinetti, J. Luthy-Hottenstein and M. Altwegg, “Value of rRNA Gene Restriction Patterns of Aeromonas spp. for Epidemiological Investigations,” Current Microbiology, Vol. 24, No. 1, 1992, pp. 15-21.
 J. Sambrook, E. F. Fritsch and T. Maniatis, “Molecular Cloning: A Laboratory Manual,” 2nd Edition, Cold Spring Harbor Laboratory, New York, 1989.
 P. C. Thomas, P. R. Divya, V. Chandrika and M. P. Paulton, “Genetic Characterization of Aeromonas hydrophila Using Protein Profiling and RAPD PCR,” Asian Fisheries Science, Vol. 22, No. 2, 2009, pp. 763-771.
 M. Nei and W. H. Li, “Mathematical Model for Studying Genetic Variation in Terms of Restriction Endonucleases,” Proceedings of the National Academy of Sciences of the United States of America, Vol. 76, No. 10, 1979, pp. 5269-5273.
 M. Miyata, T. Aoki, V. Inglis, T. Yoshida and M. Endo, “RAPD Analysis of Aeromonas salmonicida and Aeromonas hydrophila,” Journal of Applied Microbiology, Vol. 79, No. 2, 1995, pp. 181-185.
 J. I. MacInnes, T. J. Trust and J. H. Corsa, “Deoxyribonucleic Acid Relationships among Members of the Genus Aeromonas,” Canadian Journal of Microbiology, Vol. 25, No. 5, 1979, pp. 579-586. doi:10.1139/m79-083
 H. Hadrys, M. Balick and B. Schierwater, “Applications of Random Amplified Polymorphic DNA (RAPD) in Molecular Ecology,” Molecular Ecology, Vol. 1, No. 1, 1992, pp. 55-63.