Z-Scan method for measurement of bovine serum albumin concentrations
Affiliation(s)
School of Medicine, Royal College of Surgeons in Ireland-Bahrain, Busaiteen, Bahrain. Department of Basic Medical Science, Royal College of Surgeons in Ireland-Bahrain, Busaiteen, Bahrain.
School of Medicine, Royal College of Surgeons in Ireland-Bahrain, Busaiteen, Bahrain. Department of Basic Medical Science, Royal College of Surgeons in Ireland-Bahrain, Busaiteen, Bahrain.
ABSTRACT
The changes in the nonlinear refractive of Bradford reagent caused by its binding to bovine serum albumin (BSA) were investigated in aqueous solutions. The nonlinear refractive index at the wavelength of 514 nm was measured using Z-scan Technique. The presence of BSA shows an increase of the nonlinear refractive index coefficient over Bradford reagent alone. The nonlinear refractive index the BSA was found to be linearly dependent on the concentration of the BSA. The nonlinear values n2 shows extended linearity over wide range of relatively high concentration. This extended linear range can be considered as an advantage over traditional absorption at peak value.
The changes in the nonlinear refractive of Bradford reagent caused by its binding to bovine serum albumin (BSA) were investigated in aqueous solutions. The nonlinear refractive index at the wavelength of 514 nm was measured using Z-scan Technique. The presence of BSA shows an increase of the nonlinear refractive index coefficient over Bradford reagent alone. The nonlinear refractive index the BSA was found to be linearly dependent on the concentration of the BSA. The nonlinear values n2 shows extended linearity over wide range of relatively high concentration. This extended linear range can be considered as an advantage over traditional absorption at peak value.
Cite this paper
Obeid, A. , Alwatari, Y. , Culligan, K. and Henari, F. (2012) Z-Scan method for measurement of bovine serum albumin concentrations. Journal of Biomedical Science and Engineering, 5, 290-293. doi: 10.4236/jbise.2012.55037.
Obeid, A. , Alwatari, Y. , Culligan, K. and Henari, F. (2012) Z-Scan method for measurement of bovine serum albumin concentrations. Journal of Biomedical Science and Engineering, 5, 290-293. doi: 10.4236/jbise.2012.55037.
References
[1] Dhinaa, A.N. and Palanisany, P.K. (2010) Z-scan technique: To measure the total protein and albumin in blood. Journal of Biomedical Science and Engineering, 3, 285-290. doi:10.4236/jbise.2010.33038 [2] Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Chemistry, 72, 248-254. [3] Chial, H.J. and Splittgerber, A.G. (1993) A comparison of the binding of Coomassie brilliant blue to proteins at low and neutral pH. Analytical Biochemistry, 213, 362- 369. doi:10.1006/abio.1993.1433 [4] Compton, S.J. and Jones, C.G. (1985) Mechanism of dye response and interference in the Bradford protein assay. Analytical Biochemistry, 151, 369-374. doi:10.1016/0003-2697(85)90190-3 [5] Passad, P N. and Williams, D.J. (1991) Introduction to nonlinear effects in molecules and polymers, Wiley, New York, 307. [6] Henari, F.Z. and Culligan, K.G. (2010) The influence of pH on nonlinear refractive index of bromophenol blue. American Journal of Applied Sciences, 1, 27-30. [7] Shen, Y.R. (2003) The principles of nonlinear optics. John Wiley and Sons Ltd., New Jersey. [8] Sheik-Bahae, M., Said, A.A., Wei, T.H., Hagan, D.J. and Van Stryland, E.W. (1990) Sensitive measurement of optical nonlinearities using single beam. IEEE Journal of Quantum Electronics, 26, 760-769. doi:10.1109/3.53394
[1] Dhinaa, A.N. and Palanisany, P.K. (2010) Z-scan technique: To measure the total protein and albumin in blood. Journal of Biomedical Science and Engineering, 3, 285-290. doi:10.4236/jbise.2010.33038 [2] Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Chemistry, 72, 248-254. [3] Chial, H.J. and Splittgerber, A.G. (1993) A comparison of the binding of Coomassie brilliant blue to proteins at low and neutral pH. Analytical Biochemistry, 213, 362- 369. doi:10.1006/abio.1993.1433 [4] Compton, S.J. and Jones, C.G. (1985) Mechanism of dye response and interference in the Bradford protein assay. Analytical Biochemistry, 151, 369-374. doi:10.1016/0003-2697(85)90190-3 [5] Passad, P N. and Williams, D.J. (1991) Introduction to nonlinear effects in molecules and polymers, Wiley, New York, 307. [6] Henari, F.Z. and Culligan, K.G. (2010) The influence of pH on nonlinear refractive index of bromophenol blue. American Journal of Applied Sciences, 1, 27-30. [7] Shen, Y.R. (2003) The principles of nonlinear optics. John Wiley and Sons Ltd., New Jersey. [8] Sheik-Bahae, M., Said, A.A., Wei, T.H., Hagan, D.J. and Van Stryland, E.W. (1990) Sensitive measurement of optical nonlinearities using single beam. IEEE Journal of Quantum Electronics, 26, 760-769. doi:10.1109/3.53394