RAPD analysis for genetic diversity of germplasm resources of Strobilanthes
ABSTRACT
The 18 samples representing 18 populations of Strobilanthes cusia (Nees) O. Ktze in Fujian Province of China were analyzed with RAPD markers. Eleven primers were used, a total of 106 bands were scored and 88 of them were polymorphic. The percentage of polymorphic loci was 77.36%, Nei’s gene diversity was 0.2420, and Shannon’s index was 0.3700. The 18 populations were classified into 2 groups based on the RAPD data by the method of hierarchical cluster analysis. Most of the populations from Fujian were clustered into a group, other populations were clustered into the other group. There was a high level of genetic diversity among the populations, and the genetic differentiation was obvious among the populations from Fujian.
The 18 samples representing 18 populations of Strobilanthes cusia (Nees) O. Ktze in Fujian Province of China were analyzed with RAPD markers. Eleven primers were used, a total of 106 bands were scored and 88 of them were polymorphic. The percentage of polymorphic loci was 77.36%, Nei’s gene diversity was 0.2420, and Shannon’s index was 0.3700. The 18 populations were classified into 2 groups based on the RAPD data by the method of hierarchical cluster analysis. Most of the populations from Fujian were clustered into a group, other populations were clustered into the other group. There was a high level of genetic diversity among the populations, and the genetic differentiation was obvious among the populations from Fujian.
Cite this paper
Ning, S. , Zhang, Y. , Zhu, R. , Zhu, L. , Lin, J. and Wei, D. (2012) RAPD analysis for genetic diversity of germplasm resources of Strobilanthes. Agricultural Sciences, 3, 400-404. doi: 10.4236/as.2012.33047.
Ning, S. , Zhang, Y. , Zhu, R. , Zhu, L. , Lin, J. and Wei, D. (2012) RAPD analysis for genetic diversity of germplasm resources of Strobilanthes. Agricultural Sciences, 3, 400-404. doi: 10.4236/as.2012.33047.
References
[1] Anonym (1975) A collection of the medicine Heber in China. Beijing People’s Medical Publishing House, Bei-jing. [2] Qian, Y.C. (1999) Investigation of the Strobilanthes cusia (Nees) O. Ktzehas in south Zhejiang province. Lishizhen Medicine and Materia Medica Research, 10, 7-8. [3] Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (2002) Short protocols in molecular biology. 5th Edition, Wiley, New York.
[1] Anonym (1975) A collection of the medicine Heber in China. Beijing People’s Medical Publishing House, Bei-jing. [2] Qian, Y.C. (1999) Investigation of the Strobilanthes cusia (Nees) O. Ktzehas in south Zhejiang province. Lishizhen Medicine and Materia Medica Research, 10, 7-8. [3] Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (2002) Short protocols in molecular biology. 5th Edition, Wiley, New York.