GSC  Vol.2 No.2 , May 2012
A Novel Immunological Technique for Detection of Gramicidin (S) Producing Bacteria
ABSTRACT
A serological kit was prepared for the first time to detect bacteria that produce Gramicidin (S). Since, an immunocomplex of antibiotic Gramicidin (S) was prepared followed by immunonization of laboratory animales to obtain specific antibodies in their serum. Serial dilutions of the prepared antibodies were tested directly against samples of bacteria and compared with control samples, which include bacteria that produce Gramicidin (S) (as a positive control) and the bacteria that does not produce Gramicidin (S) (as a negative control) depending slide method after the incubation of bacteria in a suitable media for 3 - 4 hours at 37°C. The prepared kites were evaluated in reference Laboratories and they proved to be highly sensitive (100%), specific (100%), more economic and reliable procedure, in addition to its stability for more than one year. It was the first time to prepare a kit for detection of bacteria which produce Gramicidin (S) directly within few minutes.

Cite this paper
F. Houssein Kamel, "A Novel Immunological Technique for Detection of Gramicidin (S) Producing Bacteria," Green and Sustainable Chemistry, Vol. 2 No. 2, 2012, pp. 55-58. doi: 10.4236/gsc.2012.22009.
References
[1]   G. Nagamurthi and S. Rambhav, “Gramicidin (S): Structure-Activity Relationship,” Journal of Biosciences, Vol. 7, No. 3-4, 1985, pp. 323-329.

[2]   W. Peter, “Three-Component Synthesis of α,β-Cyclopro- pyl-γ-Amino Acids,” American Chemical Society, Divi- sion of Organic Chemistry, Vol. 127, No. 206, 2005, pp. 5742-5743.

[3]   A. Llamas-Saiz, “Double-Stranded Helical Twisted β-Sheet Channels in Crystals of Gramicidin (S) Grown in the Presence of Trifluoroacetic and Hydrochloric Acids,” Acta Crystallographica Section D, Vol. 63, No. 3, 2007, pp. 401-407. doi:10.1107/S0907444906056435

[4]   P. Brick, “Structural Basis for the Activation of Pheny- lalanine in the Non-Ribosomal Biosynthesis of Gramicidin S,” The EMBO Journal, Vol. 16, No. 14, 1997, pp. 4174-4183. doi:10.1093/emboj/16.14.4174

[5]   T. Korzybaski, Z. Kowszk and W. Kurylowicz, “Antibiotics: Origin, Nature and Properties,” Pergamon Press, Oxford, London, NewYork, 1967.

[6]   E. D. Snell and G. T. Snell, “Calorimetric Method of Analysis,” Van Nostr and Reinhold Company, Newark, Toranto, London. 1971.

[7]   E. Kate and A. L. Demaine, “The Peptide Antibiotic of Bacillus: Chemistry, Biogenesis, and Possible Functions,” Bacteriology Reviews, Vol. 41, No. 2, 1977, pp. 449-474.

[8]   S. S. LateEf, S. Gupta, L. P. Jayathlilaka, S. Krishnanchettia, J. S. Huanq and B. S. Lee, “An Improved Protocol for Coupling Synthetic Peptides to Carrier Proteins for Antibody Production Using DMF to Solubilize Peptides,” Journal of Biomolecular Techniques, Vol. 18, No. 3, 2007, pp. 173-176.

[9]   L. P. Cawley, “Electrophoresis and Immunoelectropho-resis,” Little Brown Co., Boston, 1973.

[10]   W. P. Jencks, M. R. Jetton and E. L. Durrum, “Paper Electrophoresis as a Quantitative Method. Serum Proteins,” Biochemical Journal, Vol. 60, No. 2, 1955, pp. 205-215.

[11]   A. Gavin, K. Hoebe, B. Duong, T. Ota, C. Martin, B. Beutler and D. Nemazee, “Adjuvant-Enhanced Antibody Responses in the Absence of Toll-Like Receptor Signaling,” Science, Vol. 314, No. 5807, 2006, pp. 1936-1938. doi:10.1126/science.1135299

[12]   M. J. Crumpton, “Protein Antigens: The Molecular Bases of Antigenicity and Immunogenicity,” In: M. Sela, Ed., The Antigens, Academic Press, New York, San Francisco, London, 1974, pp. 1-78.

[13]   B. Benaceraf and E. R. Unanue, Textbook of Immunology, 1969, pp. 23-29, 54-75.

[14]   F. Kurzer and K. Donraghi-Zadeh, “Advances in the Chemistry of Carbodiimides,” Chemical Reviews, Vol. 67, No. 2, 1967, pp. 107-152. doi:10.1021/cr60246a001

[15]   E. A. Kabat and M. M. Mayer, “Experimental Immuno- chemistry,” Thomas, Spring Field, 1961.

[16]   H. G. Khorana, “Observations on the Use of Dicyclo- hexylcarbodiimide in the Synthesis of Peptides,” Chemical Industries, London, 1955.

[17]   S. AL-Safi and F. H. Kamel, “Efficiency of Three Methods in Separation Highly Purified Human IgG,” Iraqi Journal of Chemistry, Vol. 19, No. 1, 1994, pp. 104-122.

[18]   P. Delves and I. Roitt, “Encyclopedia of Immunology,” 2nd Edition, Academic Press Inc., San Diego, 1999.

 
 
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