ABSTRACT Dicranostigma Leptopodum (Maxim) Fedde (DL- F), which had been previously documented to suppress oxidative hemolysis of erythrocytes and enhance immune functions of murine peri- toneal macrophages, was investigated for its effect on anti-tumor activity. Of alkaloids extracted from DLF, five have been identified with employment of chromatographic analysis. An antiproliferative role of these alkaloids was determined on SMMC-7721 Human Hepatoma Ce- lls in an apoptosis-inducing manner, through MTT assaying, Trypan blue exclusion assaying and cytometric analysis of cell cycle distribution. To further examine their inhibitory effects on tumor progression, murine H22 cells were inoculated into Kunming mice to determine the role of these alkaloids of DLF in inhibiting tumor growth in the tumor-implanted mice. It was found that these alkaloids of DLF enhanced the tumor shrinkage effectively wherein its tumor inhibitory rate and immunohistochemistry stain- ing of the tumor were determined and profiled, respectively.
Cite this paper
Zhang, W. , Lv, M. , Hai, J. , Wang, Q. and Wang, Q. (2010) Dicranostigma leptopodum (maxim) fedde induced apoptosis in SMMC-7721 human hepatoma cells and inhibited tumor growth in mice. Natural Science, 2, 457-463. doi: 10.4236/ns.2010.25056.
 Molinari, G. (2009) Natural products in drug discovery: Present status and perspectives. Advances in Experimental Medicine and Biology, 655(1), 13-27.
Ji, H.F., Li, X.J. and Zhang, H.Y. (2009) Natural products and drug discovery: Can thousands of years of ancient medical knowledge lead us to new and powerful drug combinations in the fight against cancer and dementia? EMBO Report, 10(3), 194-200.
Kong, D.X., Li, X.J., Tang, G.Y. and Zhang, H.Y. (2008) How many traditional Chinese medicine components have been recognized by modern Western medicine? A chemoinformatic analysis and implications for finding multicomponent drugs. ChemMedChem, 3(2), 233-236.
Li, J.W. and Vederas, J.C. (2009) Drug discovery and natural products: end of an era or an endless frontier? Science, 325(5937), 161-165.
Efferth, T., Li, P.C., Konkimalla, V.S. and Kaina, B. (2007) From traditional Chinese medicine to rational cancer therapy. Trends in Molecular Medicine, 13(8), 353-361
Li, X.J. and Zhang, H.Y. (2008) Western-medicine-vali- dated anti-tumor agents and traditional Chinese medicine. Trends in Molecular Medicine, 14(1), 1-2.
Wang, X., Zhang, L.H. and Wang, Q. (2006) Enhanced effect of Dicranostigma Leptopodum (Maxim.) Fedde on experimental immunosuppression in mice. Journal of Lanzhou University (Natural Sciences), 36(4), 6-12.
Zhao, Q., Han, Y., Du, Y.P., Wang, T.P. and Wang, Q. (2006) The effect of Dicranostigma Leptopodum (Maxim) Fedde (DLF) extraction on suppressing oxidative hemo-lysis of erythrocytes and its mechanism. Journal of Lanzhou University Medical Sciences, 3, 3-11.
Ruo, X., Chang, H.W. and Ma, G.G. (1981) An analysis of the chemical components of Dicranostigma Leptopo-dum (Maxim.) Fedde. Chinese Pharmaceutical Bulletin, 16(2), 118-123.
Yan Dang HG, Junxi Liu, Sijiu Yu. 2009. Alkaloid from Dicranostigma Leptopodum (Maxim) Fedde. Chinese Chemical Letters, 20(10), 1218-1220.
Copper, C.L., Newman, C.I. and Collins, G.E. (2008) Simple and rapid extraction, separation, and detection of alkaloids in beverages. Journal of Separation Science, 31(21), 3727-3731.
Chen, J., Wang, F., Liu, J., Lee, F.S., Wang, X. and Yang, H. (2008) Analysis of alkaloids in Coptis chinensis Franch by accelerated solvent extraction combined with ultra performance liquid chromatographic analysis with photodiode array and tandem mass spectrometry detec-tions. Analytical Chimica Acta, 613(2), 184-195.
Marks, D.C., Belov, L., Davey, M.W., Davey, R.A. and Kidman, A.D. (1992) The MTT cell viability assay for cytotoxicity testing in multidrug-resistant human leukemic cells. Leukemia Research, 16(12), 1165-1173.
Darlington, G.J. (2007) Viability Staining of Mammalian Cell Cultures. Cold Spring Harbor Protocols, pdb. prot4769, New York.
Winter, H.K., Ehrlich, V.A., Grusch, M., Lackner, A., Schulte-Hermann, R., et al. (2008) Use of four new human-derived liver-cell lines for the detection of geno- toxic compounds in the single-cell gel electrophoresis (SCGE) assay. Mutation Research, 657(2), 133-139.
Cai, J., Lei, L.S., Chi, D.B. (2009) Antineoplastic effect of koumine in mice bearing H22 solid tumor. Journal of Southern Medical University, 29(9), 1851-1852.
Zhu, B.D., Yuan, S.J., Zhao, Q.C., Li, X., Li, Y., Lu, Q.Y. (2005) Antitumor effect of Gefitinib, an epidermal growth factor receptor tyrosine kinase inhibitor, combined with cytotoxic agent on murine hepatocellular carcinoma. World Journal of Gastroenterology, 11(9), 1382-1386.
Herrmann, H.J. and Schlosser, G. (1974) Detection of osteoid tissues and degenerated intercellular bone sub-stance using a modification of the hematoxylin-cosin staining technique (author’s transl). Zentralbl Allgemeine Pathology, 118, 342-347.
Abe, Y., Yonemura, K., Nishida, K. and Takagi, K. (1994) Giant cell tumor of bone: analysis of proliferative cells by double-labeling immunohistochemistry with anti-pro- liferating cell nuclear antigen antibody and culture pro-cedure. Nippon Seikeigeka Gakkai Zasshi, 68(5), 407- 414.
Baker, J.T., Borris, R.P., Carte, B., Cordell, G.A., Soejarto, D.D., et al. (1995) Natural product drug discovery and development: new perspectives on international col-laboration. Journal of Natural Products, 58(9), 1325- 1357.
Itokawa, H., Morris-Natschke, S.L., Akiyama, T. and Lee, K.H. (2008) Plant-derived natural product research aim- ed at new drug discovery. Journal of Nature Medicine, 62(3), 263-280.
Belicza, M. (2009) Evaluation of morphologically deter- mined apoptotic index. Acta Medica Croatica, 63(Suppl 2), 3-12.