NS  Vol.2 No.5 , May 2010
Kinetic spectrophotometric determination of certain cephalosporins using iodate/iodide mixture
Abstract: A simple, precise and accurate kinetic spectro-photometric method for determination of ce-fradine anhydrous, cefaclor monohydrate, ce-fadroxil monohydrate, cefalexin anhydrous and cefixime in bulk and in pharmaceutical formula-tions has been developed. The method based on a kinetic investigation of the reaction of the free carboxylic acid group of the drug with a mixture of potassium iodate and potassium iodide at room temperature to form yellow coloured triiodide ions. The reaction was followed up spectrophotometrically by measuring the increase in absorbance at 352 nm as a function of time. The initial rate, fixed time, variable time and rate-constant methods were adopted for constructing the calibration curves but fixed time method has been found to be more applicable. The analytical performance of the method, in terms of accuracy and precision, was statistically validated; the results were satisfactory. The method has been successfully applied to the determination of the studied drugs in commercial pharmaceutical formulations. Statistical comparison of the results with a well established reported method showed excellent ag- reement and proved that there is no significant difference in the accuracy and precision.
Cite this paper: El-Shaboury, S. , Mohamed, F. , Saleh, G. and Rageh, A. (2010) Kinetic spectrophotometric determination of certain cephalosporins using iodate/iodide mixture. Natural Science, 2, 432-443. doi: 10.4236/ns.2010.25053.

[1]   United States Pharmacopoeia 31 and NF 26. (2008) American Pharmaceutical Association, Washington, DC.

[2]   El-Obeid, H.A., Gad-Kariem, E.A., Al-Rashood, K.A., Al-Khames, H.A., El-Shafie, F.S. and Bawaseer, G.A.M. (1999) A selective colorimetric method for the determi-nation of penicillins and cephalosporins with α-aminoacyl functions. Analytical Letters, 32(14), 2809-2823.

[3]   Metwally, F.H., Alwarthan, A.A. and Al-Tamimi, S.A. (2001) Flow-injection spectrophotometric determination of certain cephalosporins based on the formation of dyes. Il Farmaco, 56(8), 601-607.

[4]   Sastry, C.S.P., Rao, S.G., Naidu, P.Y. and Srinivas, K.R. (1998) New spectrophotometric method for the determi-nation of some drugs with iodine and wool fast blue BL. Talanta, 45(6), 1227-1234.

[5]   Ivama, V.M., Rodrigues, L.N.C, Guaratini, C.C.I and Zanoni, M.V.B. (1999) Spectrophotometric determination of cefaclor in pharmaceutical preparations. Quimica Nova, 22(2), 201-204.

[6]   Al-Momani, I.F. (2004) Flow-injection spectrophotomet-ric determination of amoxycillin, cefalexin, ampicillin, and cefradine in pharmaceutical formulations. Analytical Letters, 37(10), 2099-2110.

[7]   Yang, J., Zhou, G.J., Cao, X.H., Ma, Q.L. and Dong, J. (1998) Study on the fluorescence characteristics of alka-line degradation of cefadroxil, cefradine, cefotaxime so-dium and amoxycillin. Analytical Letters, 31, 1047-1060.

[8]   Aly, F.A., Hefnawy, M.M. and Belal, F. (1996) A selective spectrofluorimetric method for the determination of some α-aminocephalosporins in formulations and biological fluids. Analytical Letters, 29(1), 117-130.

[9]   Yang, J.H., Zhou, G.J., Jie, N.Q., Han, R.J., Lin, C.G. and Hu, J.T. (1996) Simultaneous determination of cefalexin and cefadroxil by using the coupling technique of syn-chronous fluorimetry and h-point standard additions method. Analytica Chimica Acta, 325(3), 195-200.

[10]   Yang, J.H., Ma, Q.L., Wu, X., Sun, L.M., Cao, X.H. (1999) A new luminescence spectrometry for the deter-mination of some β-lactamic antibiotics. Analytical Letters, 32(3), 471-480.

[11]   Chailapakul, O., Aksharanandana, P., Frelink, T., Einaga, Y. and Fujishima, A. (2001) The electrooxidation of sul-fur-containing compounds at boron-doped diamond elec-trode. Sensors and Actuators B, 80(3), 193-201.

[12]   Chailapakul, O., Fujishima, A., Tipthara, P. and Siri-wongchai, H. (2001) Electroanalysis of glutathione and cefalexin using the boron-doped diamond thin-film elec-trode applied to flow-injection analysis. Analytical Sci-ences, 17(ICAS2001), i417-i422.

[13]   Li, Q.L. and Chen, S. (1993) Studies on electrochemical behaviour of cefalexin. Analytica Chimica Acta, 282(1), 145-152.

[14]   Crouch, S.R., Cullen, T.F., Scheeline, A. and Kirkor, E.S. (1998) Kinetic determinations and some kinetic aspects of analytical chemistry. Analytical Chemistry, 70(12), 53R-106R.

[15]   Perez-Bendito, D., Gomez-Hens, A. and Silva, M. (1996) Advances in drug analysis by kinetic methods. Journal of Pharmaceutical and Biomedical Analysis, 14(8-10), 917-930.

[16]   Espinosa-Mansilla, A., Acedo Valenzuela, M.I., Salinas, F. and Canada, F. (1998) Kinetic determination of ansamicins in pharmaceutical formulations and human urine; manual and semiautomatic (stopped-flow) procedures. Analytica Chimica Acta, 376(3), 365-375.

[17]   Helaleh, M.I.H. and Abu-Nameh, E.S.M. (1998) A kinetic approach for determination of cefadroxil in phar-maceuticals by alkaline hydrolysis. Journal of AOAC In-ternational, 81(3), 528-533.

[18]   Rahman, N., Ahmad, Y. and Azmi, S.N.H. (2005) Kinetic spectrophotometric method for the determination of ramipril in pharmaceutical formulations. AAPS Pharm- SciTech, 6(3), E543-E551.

[19]   Neil, S.I. (1987) Physical organic chemistry. John Wiley & Sons, New York, 93.

[20]   Kelly, F.C. (1953) Studies on the stability of iodine compounds in iodized salt. Bulletin of World Health Or-ganization, 9(2), 217-230.

[21]   Yatsimirskii, K.B. (1966) Kinetic methods of analysis. Pergamon Press, London, 43.

[22]   Laitinen H.A., Harris, W.E. (1975) Chemical analysis. 2nd Edition, McGraw-Hill, New York.

[23]   (2005) Topic Q2 (R1): Validation of analytical procedures: text and me thodology. International Conference on Harmonization, Foster.

[24]   Saleh, G.A., Askal, H., Darwish, I. and El-Shorbagi, A. (2003) Spectroscopic analytical study for the charge- transfer complexation of certain cephalosporins with chloranilic acid. Analytical Sciences, 19(2), 281-287.

[25]   Harvey, D. (2000) Modern analytical chemistry. Boston, McGraw-Hill, Massachusetts, 108.

[26]   The Merck index (2001) An encyclopedia of chemicals, drugs and pharmaceuticals. 13th Edition, Merck & Co., INC., New Jersey, 133.

[27]   Svehla, G. (1979) Vogel’s textbook of macro and semi- micro qualitative inorganic analysis. 5th Edition, the Chaucer Press, Great Britain, 342.