A simple, economic, and time-efficient related substance, GC method has been developed for the analysis of 1,4-Cyclohexanedione mono-
ethylene ketal(will be specified as ketal) in the presence of a potential impurity 1,4-cyclohexa-
nedione bis (ethylene ketal) [will be specified as diketal]. Successful chromatographic separa- tion of the ketal from the impurity was achieved on a DBWAX ETR, 30 m x 0.32 mm x 1.0μ FT column with nitrogen as carrier gas and FID detector. The method was validated for linearity, accuracy, precision, and specificity and can be used for quality control during manufacture of ketal. A validated GC method is reported for the ketal for the first time.
Cite this paper
nullKarthikeyan, B. and Srinivasan, V. (2010) A validated, related substance, GC method for 1,4-cyclohexanedione mono-ethylene Ketal. Journal of Biophysical Chemistry, 1, 72-75. doi: 10.4236/jbpc.2010.11009.
 Mussini, P., Orsini, F. and Pelizzoni, F. (1975) A prepara-tion of the ethylene glycol monoketal of cyclohexane-1, 4-dione. Synthetic Communications, 5(4), 283-286.
 Huet, F., Pellet, M. and Conia, J. M. (1976) α-Diketone- monoketals and-monothioketals. Tetrahedron Letters, 17 (39), 3579- 3582.
 Huet, F., Pellet, M. and Conia, J. M. (1976) α-cyclopropylidene-ketals-ketones and aldehydes. Tetra-hedron Letters, 18(29), 2521-2524.
 Nangia, A. and Chandrakala, P. S. (1996) Experimental and computational studies on the hydrolysis rate of ethy-lene ketals in 1,3-cyclohexenediones. Journal of the Chemical Society, 108(1), 51-56.
 Volpe, T., Revial, G., Pfau, M. and dAnjelo, J. (1986) Synthesis of 2-methyl-1,3, cyclopentane dione monoe-thylene ketal. Tetrahedron Letters, 27(25), 2853-2854,.
 Miriyala, B. Bhattacharyya, S. and Williamson, J. S. (2004) Chemoselective reductive alkylation of ammonia with carbonyl compounds: synthsis of primary and sym-metrical secondary amines. Tetrahedron, 60, 1463-1471,.
 Balko, T. W., Fields, S. C. and Webster, J. D. (1999) Total synthesis of (+)-8′-trifluoromethyl abscisic acid. Tetrahe-dron Letters, 40(35), 6347-6351.
 Torbeck, L. (2002) Complying with ICH and FDA re-quirements for assay validation. Suffield press, Evanston.
 (2006) International conferences on Harmonization. Draft revised guidance on impurities in new drug substances, Q3A(R2).
 (1994) International Conferences on Harmonization. Validation of analytical procedures, Q2(R).