ABSTRACT The patterns of effective pollen dispersal and the relationships between pollen dispersal and genetic composition in Pinus koraiensis are still unclear. Hence, we investigated the mode of pollen dispersal of P. koraiensis in a clonal seed orchard in Lushuihe Forestry Bureau, Jilin Province, using SSR molecular markers tech-nique and the method of maximum likelihood. A total of 13 pairs of nuclear microsatellites po-lymorphic primers were used in the paternity analysis. We analyzed 100 progenies and 150 paternities. A total of 56 alleles were detected in 13 loci with 3-6 alleles (4.3 alleles in average) in a single locus. The primers of SsrPt_ctg7170 and SsrPt_ctg5333 had the maximum (6) and the minimum (3) alleles, respectively. The averaged values of observed heterozygosity, expected heterozygosity and polymorphism information content of experimental groups were 0.648, 0.601 and 0.533, respectively. The mating dis-tance of P. koraiensis followed an approximately normal distribution. The most effective pollen for the female parent came from male parent trees 15-45 m away with an average mating dis-tance of 32.60 m and the longest of 67.88 m. The pollen source of offspring was not random but greatly affected by the wind direction during the pollination season of P. koraiensis. These re-sults have important implications for seed or-chard design to improve the genetic quality of seeds and seed production.
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nullFeng, F. , Sui, X. , Chen, M. , Zhao, D. , Han, H. and Li, M. (2010) Mode of pollen spread in clonal seed orchard of Pinus koraiensis. Journal of Biophysical Chemistry, 1, 33-39. doi: 10.4236/jbpc.2010.11004.
 Ma, J.L., Zhang, L.W., Cheng D. and Li, J.W. (1992) Geographic distribution of Pinus koraiensis in the world. Journal of Northeast Forestry University, 20, 40-47.
 Levin, D.A. and Kerster, H.W. (1974) Gene flow in seed plants. Evolutionary Biology, 7, 139-220.
 Hamrick, J.L. and Schnabel, A. (1985) Understanding the genetic structure of plant populations: Some old problems and a new approach. In: Gregorius, H.R., Ed., Population Genetics in Forestry, Springer-Verlag, New York, 5, 0-70.
 Adams, W.T., Birkes, D.S. and Erickson, V.J. (1992) Using genetic markers to measure gene flow and pollen dispersal in forest tree seed orchards. In: Wyatt, R., Ecology and Evolution of Plant Reproduction: New Ap-proaches, Chapman and Hall, New York.
 Meagher, T.R. (1986) Analysis of paternity within a nat-ural population of Chamaelirium leuteum. I. Identification of most-likely male parents. American Naturalist, 128(2), 199-215.
 Snow, A.A. and Lewis, P.O. (1993) Reproductive traits and male fertility in plants Empirical approaches. Annual Review of Ecology and Systematics, 24, 331-351.
 Adams, W.T. and Birkes, D.S. (1989) Matting patterns in seed orchards. Proceedings of 20th Southern Forest Tree Improvement Conference, Charleston, 26-30 June 1989, 75-86.
 Wheeler, N.C. and Jech, K.S. (1992) The use of electro-phoretic markers in seed orchard research. New Forests, 6(1-4), 311-328.
 Lian, C.L., Miwa, M. and Hogetsu, T. (2001) Outcrossing and paternity analysis of Pinus densiflora (Japanese red pine) by microsatellite polymorphism. The Genetics Society of Great Britain, 87, 88-98.
 Kong, F.L. (2006) Quantitative genetics in plants. China Agricultural University Press, Beijing, 20-50.
 Wang, J., Yang C.P. and Liu, G.F. (2006) Extraction of genomic DNA from woody plants and it’s identification. Bulletin of Botanical Research, 26, 589-594.
 Zhou, Y., Bui, T., Auckland, L.D. and Williams, C.G. (2002) Undermethylated DNA as a source of microsatel-lites from a conifer genome. Genome, 45(1), 91-99.
 Chagné, D., Chaumeil, P., Ramboer, A., Collada, C., Guevara, A., Cervera, M.T., Vendramin, G.G., Garcia, V., Frigerio, J.M., Echt, C., Richardson, T. and Plomion, C. (2004) Cross-species transferability and mapping of genomic and cDNA SSRs in pines. Theoretical and Applied Genetics, 109(6), 1204-1214.
 Liewlaksaneeyanawin, C., Ritland, C.E., EI-Kassaby, Y.A. and Ritland, K. (2004) Single-copy, species-transferable microsatellite markers developed from loblolly pine ESTs. Theoretical and Applied Genetics, 109(2), 361-369.
 Ge, S. (1998) Isozymes and studies on population genetic variation in forest trees. Journal of Nanjing Forest Uni-versity, 1, 68-77.
 El-Kassaby, Y.A. (1991) Genetic variation within and among conifer populations: review and ecaluation of methods. In: Fineschi, S., Ed., Biochemical Markers in the Population Genetics of Forest Trees, SPB Academic Publishing, Hague, 61-76.
 Nei, M. (1972) Genetic distance between populations. American Naturalist, 106, 283-292.
 Botstein, D., White, R.L., Skolnick, M. and Davis, R.W. (1980) Construction of a genetic linkage maps in man using restriction fragment length polymorphisms. Ameri-can Journal of Human Genetics, 32(3), 314-331.
 Smouse, P.E. and Meagher, T.R. (1994) Genetic analysis of male reproductive contributions in Chamaelirium lu-teum (L.) gray (Liliaceae). Genetics, 136(1), 313-322.
 Chen, X.Y. and Song, Y.C. (2000) Analysis of parentage for naturally for naturally established seedlings of cyclo- balanopsis glauca. Journal of Wuhan Botanical Research, 18, 174-180.
 Sun, Y.G. and Li, H.G. (2007) The paternity analysis for open-pollination progenies of Liriodendron L. using SSR markers. Chinese Bulletin of Botany, 24, 590-596..
 Zhang, D.M., Sun, P.G., Shen, X.H. and Ru, G.X. (2009) Paternity analysis of open-and control-pollinated seeds collected from a seed orchard of Pinus tabulaeformis. Chinese Journal of Plant Ecology, 33(2), 302-310.
 He, T.H. and Ge, S. (2001) Mating system, paternity analysis and gene flow in plant populations. Acta Phy-toecologica Sinica, 25, 144-154..
 Sulaiman, Z., Collins, G., Witherspoon, J. and Sedgley, M. (2004) Identification of pollen donors for the avocado cultivar Gwen in a mixed orchard by isozyme analysis. Journal of Horticultural Science and Biotechnology, 79(4), 571-575.
 Wang, G.Y., Yi, H.B., Wang, B., Zhang, S.F., Wang, G.X., Huang, Q.C., Zhang, G.T. and Sun, Q.Y. (1995) Study on the pollen density in the seed orchard of Korean Pine. Journal of Northeast Forestry University, 23, 58-63.
 Stern, K. and Roche, L. (1974) Genetics of Forest Eco-systems. Springer-Verlag, Berlin, 65-69.
 Friedman, S.T. and Adams, W.T. (1985) Estimation of gene flow into two seed orchards of loblolly pine (Pinus taeda L.). Theoretical and Applied Genetics, 69(5-6), 609- 615.
 Squillace, A.E. and Long, E.M. (1982) Proportion of pol-len from nonorchard sources. In: Franklin, E.C., Ed., Pollen Management Handbook, USDA Agriculture Hand- book # 587, AVI Publishing Company Inc., Washington DC, 15-19.
 Lai, H.L. and Wang, Z.R. (1997) A study on gene flow between a Masson pine seed orchard and a plantation near the orchard. Journal of Nanjing Forestry University, 21, 37-41.
 Harju, A. and Muona, O. (1989) Background pollination in Pinus sylvestris seed orchards. Scandinavian Journal of Forest Research, 4(1-4), 513-520.
 Wang, X.R., Lindgren, D., Szmidt, A.E. and Yazdani, R. (1991) Pollen migration into a seed orchard of Pinus syl-vestris and the methods of its estimation using allozyme markers. Scandinavian Journal of Forest Research, 6(1-4), 379-386.