AJPS  Vol.3 No.4 , April 2012
Examination of Potential Measures of Vine Maturity in Potato
Abstract: Plant maturity is a complex physiological trait routinely evaluated by plant breeders because of its agronomic, economic, and breeding implications. In potato, plant maturity is typically estimated by monitoring vine characteristics. This study investigates several reported measures of vine maturity in potato cultivars, including examination of flower development, leaf chlorophyll content, and leaf peroxidase activity to see what method is most appropriate for maturity classification in temperate production regions. These three measures were evaluated multiple times throughout a single growing season across two locations. The data were analyzed using canonical discriminant and dichotomous tree analyses. Both methods revealed that flower development is not an accurate indicator of maturity, even though it is a common component of maturity screening protocols. Peroxidase measures were slightly better, but the optimal period of time to assess this trait was variable across locations and a considerable time commitment is required to collect and process samples. Monitoring leaf chlorophyll content throughout plant development was most appropriate for predicting vine maturity under these conditions, as this trait showed the most consistency and greatest prediction accuracy (69% - 71%) relative to peroxidase activity and flowering development. Additionally, chlorophyll measurements are a more practical method of measuring maturity due to the ease of data collection. Leaf chlorophyll content best distinguished late cultivars from early-medium and medium-late cultivars. However, it did not separate early-medium from medium-late cultivars. Combining chlorophyll monitoring with peroxidase and flowering measures improved the ability to distinguish among the early-medium and medium-late maturity classes. However, doing so only increased classification accuracy by 3%.
Cite this paper: E. Haga, B. Weber and S. Jansky, "Examination of Potential Measures of Vine Maturity in Potato," American Journal of Plant Sciences, Vol. 3 No. 4, 2012, pp. 495-505. doi: 10.4236/ajps.2012.34059.

[1]   R. E. Voss, “Potato,” In: The Agricultural Handbook Number 66: The Commercial Storage of Fruits, Vegetables, and Florist and Nursery Stocks, United States Department of Agriculture-Agricultural Research Services (USDA-ARS), 2005.

[2]   D. R. Douglas and J. J. Pavek, “Screening Potatoes for Field Resistance to Early Blight,” American Potato Journal, Vol. 49, No. 1, 1972, pp. 1-9. doi:10.1007/BF02862935

[3]   C. A. Bormann, et al., “Tagging Quantitative Trait Loci for Maturity-Corrected Late Blight Resistance in Tetraploid Potato with PCR-Based Candidate Gene Markers,” Molecular Plant Microbe Interactions, Vol. 17, No. 10, 2004, pp. 1126-1138. doi:10.1094/MPMI.2004.17.10.1126

[4]   A. Johanson and H. D. Thurston, “The Effect of Cultivar Maturity on the Resistance of Potatoes to Early Blight Caused by Alternaria solani,” American Potato Journal, Vol. 67, No. 9, 1990, pp. 615-623. doi:10.1007/BF03043447

[5]   J. Sliwka, et al., “Tagging QTLs for Late Blight Resistance and Plant Maturity from Diploid Wild Relatives in a Cultivated Potato (Solanum tuberosum) Background,” Theoretical and Applied Genetics, Vol. 115, No. 1, 2007, pp. 101-112. doi:10.1007/s00122-007-0546-9

[6]   S. H. Jansky, A. Hamernik and P. C. Bethke, “Germplasm Release: Tetraploid Clones with Resistance to Cold-Induced Sweetening,” American Journal of Potato Re- search, Vol. 88, No. 3, 2010, pp. 218-225. doi:10.1007/s12230-011-9186-3

[7]   P. C. Struik, M. H. P. W. Visker, J. B. Pauwels and L. T. Colon, “Are Cuttings Suitable for Assessing Maturity Type in Potato (Solanum tuberosum)?” Annals of Applied Biology, Vol. 147, No. 1, 2005, pp. 27-34. doi:10.1111/j.1744-7348.2005.00008.x

[8]   H. J. van Eck, “Localisation of Morphological Traits on the Genetic Map of Potato Using RFLP and Isozyme Markers,” Thesis, Landbouw universiteit, Wageningen, 1995.

[9]   I. Simko, “Comparative Analysis of Quantitative Trait Loci for Foliage Resistance to Phytophthora infestans in Tuber-Bearing Solanum Species,” American Journal Potato Research, Vol. 79, 2002, pp. 125-132. doi:10.1007/BF02881521

[10]   R. Zhang, “Genetic Characterization and Mapping of Par- tial Resistance to Early Blight in Diploid Potato,” Ph.D. Thsis, Pennsylvania State University, University Park, 2004, p. 158.

[11]   M. H. P. W. Visker, L. C. P Keizer, H. J. Van Eck, E. Jacobsen, L. T. Colon and P. C. Struik, “Can the QTL for Late Blight Resistance on Potato Chromosome 5 Be Attributed to Foliage Maturity Type,” Theoretical and Applied Genetics, Vol. 106, No. 2, 2003, pp. 317-325.

[12]   M. H. P. W. Visker, et al., “Correlation between Late Blight Resistance and Foliage Maturity Type in Potato,” Euphytica, Vol. 137, No. 3, 2004, pp. 311-323. doi:10.1023/B:EUPH.0000040451.21852.d8

[13]   A. L. Adám, C. S. Bestwick, B. Barna and J. W. Mansfield, “Enzymes Regulating the Accumulation of Active Oxygen Species during the Hypersensitive Reaction of Bean to Pseudomonas syringae pv. Phaseolicola,” Planta, Vol. 197, No. 2, 1995, pp. 240-249.

[14]   S. K. Sandhu, et al., “Peroxidase as a Biochemical Marker of Maturity Levels in Potato (Solanum tuberosum) Cultivars under Short Days,” New Zealand Journal of Crop and Horticultural Science, Vol. 35, No. 1, 2007, pp. 171-175. doi:10.1080/01140670709510181

[15]   C. P. Malik and M. B. Singh, “Plant Enzymology and Histo-Enzymology: A Text Manual,” Kalyani Publishers, New Delhi, India, 1980, p. 434.

[16]   T. Janda, G. Szalai, O. V. Rios-Gonzalez and E. Paldi, “Comparative Study of Frost Tolerance and Antioxidant Activity in Cereals,” Plant Science, Vol. 164, No. 2, 2003, pp. 301-306. doi:10.1016/S0168-9452(02)00414-4

[17]   J. Fu, B. Huang and G. Zhang, “Physiological and Bio- chemical Changes during Seed Filling in Relation to Leaf Senescence in Soybean,” Biologia Plantarium, Vol. 43, No. 4, 2000, pp. 545-548.

[18]   E. E. Ewing, “Cuttings as Simplified Models of the Potato Plant,” P. H. Li, Ed., Potato Physiology, Academic Press, Orlando, 1985, pp. 153-207.

[19]   J. J. McGrady and E. E. Ewing, “Potato Cuttings as Models to Study Maturation and Senescence,” Potato Research, Vol. 33, No. 1, 1990, pp. 97-108. doi:10.1007/BF02358134

[20]   P. L. Minotti, D. E. Halseth and J. B. Sieczka, “Field Chlorophyll Measurements to Assess the Nitrogen Status of Potato Varieties,” HortScience, Vol. 29, No. 12, 1994, pp. 1497-1500.

[21]   E. J. Botha, B. J. Zebarth and B. Leblon, “Non-Destructive Estimation of Potato Leaf Chlorophyll and Protein Contents from Hyperspectral Measurements Using the Prospect Radiative Transfer Model,” Canadian Journal of Plant Science, Vol. 86, 2006, pp. 279-291. doi:10.4141/P05-017

[22]   R. E. Webb, et al., “Atlantic: A New Potato Variety with High Solids, Good Processing Quality, and Resistance to Pests,” American Potato Journal, Vol. 55, No. 3, 1978, pp. 141-145. doi:10.1007/BF02852087

[23]   R. L. Plaisted, et al., “Pike: A Full Season Scab and Golden Nematode Resistant Chipstock Variety,” American Journal of Potato Research, Vol. 75, No. 3, 1998, pp. 117-120. doi:10.1007/BF02895845

[24]   J. Stark, D. Westermann and L. T. Hopkins, “Nutrient Management Guidelines for Russet Burbank Potatoes,” Extension Bulletin 840, Moscow, University of Idaho Extension, Idaho, 2004.

[25]   W. Hamester and U. Hils, “World Catalogue of Potato Varieties,” Agrimedia GmbH, Bergen, Germany, 2003.

[26]   A. L. Tek, W. R. Stevenson, J. P. Helgeson and J. Jiang, “Transfer of Tuber Soft Rot and Early Blight Resistances from Solanum brevidens into Cultivated Potato,” Theoretical and Applied Genetics, Vol. 109, No. 2, 2004, pp. 249-254. doi:10.1007/s00122-004-1638-4

[27]   E. J. Botha, B. Leblon, B. Zebarth and J. Watmough, “Non-Destructive Estimation of Potato Leaf Chlorophyll from Canopy Hyperspectral Reflectance Using the Inverted Prosail Model,” International Journal of Applied Earth Observation and Geoinformation, Vol. 9, No. 4, 2007, pp. 360-374. doi:10.1016/j.jag.2006.11.003

[28]   A. Ierna, “Characterization of Potato Genotypes by Chlorophyll Fluorescence during Plant Aging in a Mediterranean Environment,” Photosynthetica, Vol. 45, No. 4, 2007, pp. 568-575. doi:10.1007/s11099-007-0097-y

[29]   R Development Core Team, “R: A Language and Environment for Statistical Computing,” R Foundation for Statistical Computing, ISBN 3-900051-07-0, Vienna, Austria, 2008.

[30]   SAS? 9.2 Enhanced Logging Facilities, SAS Institute Inc., Cary, NC, USA Copyright ? 2008.

[31]   C. Navarro, J. A. Abelenda, E. Cruz-Oro, C. A. Cuellar, S. Tamaki, J. Silva, K. Shimamoto and S. Prat, “Control of Flowering and Storage Organ Formation in Potato by Flowering Locus T,” Nature, Vol. 478, No. 7367, 2011, pp. 119-123. doi:10.1038/nature10431

[32]   The Potato Genome Sequencing Consortium, “Genome Sequence and Analysis of the Tuber Crop Potato,” Nature, Vol. 475, No. 7355, 2011, pp. 189-195. doi:10.1038/nature10158