AJPS  Vol.3 No.4 , April 2012
Biological Control Potential of Colletotrichum gloeosporioides for Coffee Senna (Cassia occidentalis)
Abstract: A fungal pathogen, Colletotrichum gloeosporioides was isolated from a greenhouse-grown seedling of coffee senna (Cassia occidentalis) and evaluated as a mycoherbicide for that weed. Host range tests revealed that coffee senna, wild senna (C. marilandica), and sicklepod (C. obtusifolia) were also affected by this pathogen, but 35 other crop and weed species, representing 8 botanical families were not affected. The fungus sporulated prolifically on solid and liquid media with maximum spore germination and growth occurring at 20°C - 30°C. Optimal environmental conditions included at least 12 h of free moisture (dew) at 20°C - 30°C. Spray mixtures containing approximately 1.0 × 105 or more conidia·ml–1 gave maximum control when coffee senna seedlings were sprayed until runoff occurred. Coffee senna seedlings that were in the cotyledon to first-leaf growth stage were most susceptible to this pathogen. Weed control efficacy studies under field conditions demonstrated that control of coffee senna was directly proportional to the inoculum concentration applied. Results of these tests suggest that this fungus has potential as a mycoherbicide to control coffee senna, a serious weed in the southeastern U.S.
Cite this paper: C. Boyette, R. Hoagland, M. Weaver and K. Stetina, "Biological Control Potential of Colletotrichum gloeosporioides for Coffee Senna (Cassia occidentalis)," American Journal of Plant Sciences, Vol. 3 No. 4, 2012, pp. 430-436. doi: 10.4236/ajps.2012.34052.

[1]   P. Bruere, “A Coffee Substitute, Cassia occidentalis, That Is Toxic before Roasting,” Pharmaceutical Chemistry Journal, Vol. 9, No. 2, 1942, pp. 321-324.

[2]   H. L. Tookey and Q. Jones, “New Sources of Water-Soluble Seed Germs,” Economic Botany, Vol. 10, No. 2, 1965 pp. 165-174. doi:10.1007/BF02862828

[3]   D. H. Teem, C. S. Hoverland and G. A. Buchanan, “Sicklepod (Cassia obtusifolia) and Coffee Senna (Cassia occidentalis): Geographic Distribution, Germination, and Emergence,” Weed Science, Vol. 28, No. 1, 1980, pp. 68-71.

[4]   C. D. Elmore, “Weed Survey: Southern States,” Proceedings of the Southern Weed Science Society, Vol. 42, 1989, pp. 408-420.

[5]   T. M. Webster, “Weed Survey—Southern States: Broad-leaf Crops Subsection,” Proceedings of the Southern Weed Science Society, Vol. 54, 2001, pp. 244-259.

[6]   T. M. Webster and G. E. MacDonald, “A Survey of Weeds in Various Crops in Georgia,” Weed Technology, Vol. 15, No. 4, 2001, pp. 771-790. doi:10.1614/0890-037X(2001)015[0771:ASOWIV]2.0.CO;2

[7]   D. W. Hall, V. V. Vandiver and J. A. Ferrell, “Weeds in Florida (SP 37), Coffee Senna, Senna occidentalis L.,” Institute of Food and Agricultural Sciences, University of Florida, 2009.

[8]   J. Grichar, “Weed Control Issues and Update,” Texas Cooperative Extension Service, Peanut Progress, Vol. 1, No. 4, 2007, p. 2.

[9]   S. M. Brown and D. C. Bridges, “Comparative Biology and Control of Sicklepod and Coffee Senna,” Proceedings of the Southern Weed Science Society, Vol. 42, 1989, p. 111.

[10]   J. M. Higgins, R. H. Walker and T. Whitwell, “Coffee Senna (Cassia occidentalis) Competition with Cotton (Gossypium hirsutum),” Weed Science, Vol. 34, No. 1, 1986, pp. 52-56.

[11]   J. K. Norsworthy and M. J. Oliveira, “Coffee Senna (Cassia occidentalis) Germination and Emergence Is Affected by Environmental Factors and Seeding Depth,” Weed Science, Vol. 53, No. 5, 2005, pp. 657-662. doi:10.1614/WS-04-209R.1

[12]   F. E. Dayan, J. D. Weete and H. G. Hancock, “Physiological Basis for Differential Sensitivity to Sulfentrazone by Sicklepod (Senna obtusifolia) and Coffee Senna (Cassia occidentalis),” Weed Science, Vol. 44, No. 1, 1996, pp. 12-17.

[13]   D. L. Jordan, J. W. Wilcut and J. S. Richburg III, “DPX-PE350 for Weed Control in Peanut (Arachis hypogaea L.),” Peanut Science, Vol. 20, No. 2, 1993, pp. 97-101. doi:10.3146/i0095-3679-20-2-8

[14]   A. Keeton, E. C. Murdock, G. S. Stapleton and J. E. Toler, “Chemical Control Systems for Coffee Senna (Cassia occidentalis) in Cotton (Gossypium hirsutum),” Weed Technology, Vol. 10, No. 3, 1996, pp. 550-555.

[15]   R. Charudattan, “The Mycoherbicide Approach with Plant Pathogens,” In: D. O. TeBeest, Ed., Microbial Control of Weeds, Chapman and Hall, New York, 1991, pp. 24-57. doi:10.1007/978-1-4615-9680-6_2

[16]   C. D. Boyette and J. R. McAlpine, “Herbicidal Control of Sicklepod and Coffee Senna with Colletotrichum gloeosporioides,” US Patent No. 5,529,773, 1996.

[17]   C. D. Boyette, “Adjuvants Enhance the Biological Control Potential of an Isolate of Colletotrichum gloeosporioides for Biological Control of Sicklepod (Senna obtusifolia),” Biocontrol Science and Technology, Vol. 16, No. 10, 2006, pp. 1057-1066. doi:10.1080/09583150600828692

[18]   C. D. Boyette, R. E. Hoagland and M. A. Weaver, “Effect of Row Spacing on Biological Control of Sicklepod (Senna obtusifolia) with Colletotrichum gloeosporioides,” Biocontrol Science and Technology, Vol. 17, No. 9, 2007, pp. 957-967. doi:10.1080/09583150701553157

[19]   C. E. Windels, P. M. Burnes and T. Kommendahl, “Five-Year Preservation of Fusarium Species in Silica Gel and Soil,” Phytopathology, Vol. 78, No. 1, 1988, pp. 107-109. doi:10.1094/Phyto-78-107

[20]   J. Tuite, “Plant Pathological Methods: Fungi and Bacteria,” Burgess Press, Minneapolis, 1969, 239 Pages.

[21]   J. E. M. Mordue, “CMI Descriptions of Pathogenic Fungi and Bacteria,” No. 315, 1971.

[22]   J. T. Daniel, G. E. Templeton, R. J. Smith Jr. and W. T. Fox, “Biological Control of Northern Jointvetch in Rice with an Endemic Fungal Disease,” Weed Science, Vol. 21, No. 4, 1973, pp. 303-307.

[23]   C. D. Boyette, G. E. Templeton and R. J. Smith, “Control of Winged Water Primrose (Jussiae decurrens) and Northern Jointvetch (Aeshynomene virginica) with Fungal Pathogens,” Weed Science, Vol. 27, No. 2, 1979, pp. 497-501.

[24]   C. D. Boyette, “Control of Hemp Sesbania with a Fungal Pathogen, Colletotrichum truncatum,” US Patent No. 5,304,328, 1991.

[25]   C. D. Boyette, P C Quimb, Jr., C. T. Bryson, G. H. Egley and F. E. Fulgham, “Biological Control of Hemp Sesbania (Sesbania exaltata) under Field Conditions with Colletotrichum truncatum Formulated in an Emulsifiable Invert,” Weed Science, Vol. 41, No. 3, 1993, pp.496-499.

[26]   J. Cardina, R. H. Littrell and R. T. Hanlin, “Anthracnose of Florida Beggarweed (Desmodium tortussum) by (Colletotrichum truncatum),” Weed Science, Vol. 36, No. 3, 1988, pp. 329-334.

[27]   T. L. Kirkpatrick and G. E. Templeton, “Potential of Colletotrichum malvarum for Biological Control of Prickly Sida,” Plant Disease, Vol. 66, No. 4, 1982, pp. 323-325. doi:10.1094/PD-66-323

[28]   L. A. Wymore, C. Poirier, A. K. Watson and A. R. Gotlieb, “Colletotrichum coccodes, a Potential Bioherbicide for Control of Velvetleaf (Abutilon theophrasti),” Plant Disease, Vol. 72, No. 6, 1988, pp. 534-538. doi:10.1094/PD-72-0534

[29]   A. J. Wapshire, “A Strategy for Evaluating the Safety of Organisms for Biological Weed Control,” Annals of Applied Biology, Vol. 77, No. 2, 1974, pp. 201-211. doi:10.1111/j.1744-7348.1974.tb06886.x

[30]   P. C. Quimby Jr., F. E. Fulgham, C. D. Boyette and W. J. Connick Jr., “An Invert Emulsion Replaces Dew in Biocontrol of Sicklepod—A Preliminary Study,” In: D. A. Hovde and G. B. Beestman, Eds., Pesticide Formulations and Application System, Vol. 8, 1989, ASTM-STP 980. American Society for Testing Materials, Philadelphia, pp. 264-270.

[31]   P. C. Quimby Jr., N. K. Zidak, C. D. Boyette and W. E. Grey, “A Simple Method for Stabilizing and Granulating Fungi,” Biocontrol Science and Technology, Vol. 9, No. 1, 1999, pp. 5-8. doi:10.1080/09583159929857

[32]   P.C. Quimby Jr., A. J. Caesar, J. L. Birdsall, W. J. Connick Jr., C. D. Boyette, N. K. Zidack and W. E. Grey, “Granulated Formulation and Method for Stabilizing Biocontrol Agents,” US Patent No. 6,455,036, 2002.