Studies on the optimal culture conditions of Aureobasidium pullulans to produce exopolysaccharides
Affiliation(s)
Life Science College, Dalian Nationalities University, Dalian, China. Environment and Resources College, Dalian Nationalities University, Dalian, China. Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China. Nanjing Agricultural University, Nanjing, China.
Life Science College, Dalian Nationalities University, Dalian, China. Environment and Resources College, Dalian Nationalities University, Dalian, China. Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China. Nanjing Agricultural University, Nanjing, China.
ABSTRACT
In the current study, in order to change the permeability of cell membrane and solve the problem of linked group of fungi mycelium, the method of adjusting osmotic pressure of medium and adding tween-80 was established. The utilized strain with relatively high exopolysaccharide (EPS) yield and low pigment level was obtained after the rejuvenation and sifting of long-preserved Aureobasidium pullulans strain. The optimal proportion of substrate was determined by means of orthogonal test. The transformation ratio of EPS was increased by 10% - 20% and the pigment content was greatly reduced. The fermenting liquor is between creamy white and pale yellow, and the white primary product can be gained without decolourization step. Furthermore, to magnify to 5 L bioreactor can get the similar result.
In the current study, in order to change the permeability of cell membrane and solve the problem of linked group of fungi mycelium, the method of adjusting osmotic pressure of medium and adding tween-80 was established. The utilized strain with relatively high exopolysaccharide (EPS) yield and low pigment level was obtained after the rejuvenation and sifting of long-preserved Aureobasidium pullulans strain. The optimal proportion of substrate was determined by means of orthogonal test. The transformation ratio of EPS was increased by 10% - 20% and the pigment content was greatly reduced. The fermenting liquor is between creamy white and pale yellow, and the white primary product can be gained without decolourization step. Furthermore, to magnify to 5 L bioreactor can get the similar result.
Cite this paper
Ma, J. , Jiang, G. , Yao, S. , Jin, H. and Wang, C. (2012) Studies on the optimal culture conditions of Aureobasidium pullulans to produce exopolysaccharides. Journal of Biomedical Science and Engineering, 5, 203-207. doi: 10.4236/jbise.2012.54027.
Ma, J. , Jiang, G. , Yao, S. , Jin, H. and Wang, C. (2012) Studies on the optimal culture conditions of Aureobasidium pullulans to produce exopolysaccharides. Journal of Biomedical Science and Engineering, 5, 203-207. doi: 10.4236/jbise.2012.54027.
References
[1] Leathers, T.D. (2003) Biotechnological production and applications of pullulan. Applied Microbiology and Bio-technology, 62, 468-473. doi:10.1007/s00253-003-1386-4 [2] Lee, J.H., Kim, J.H., Zhu, I.H., Zhan, X.B., Lee, J.W., Shin, D.H., et al. (2001) Optimization of conditions for the production of pullulan and high molecular weight pullulan by Aureobasidium pullulans. Biotechnology Letters, 23, 817-820. doi:10.1023/A:1010365706691 [3] Shingel, K.I. (2004) Current knowledge on biosynthesis, biological activity, and chemical modification of the exopolysaccharide, pullulan. Carbohydrate Research, 339, 447-460. doi:10.1016/j.carres.2003.10.034 [4] Thibault, J. and LeDuy, A. (1999) Pullulan, microbial production methods. In: Flickinger, M.C. and Drew, S.W., Eds., Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation, John Wiley & Sons, New York, 2232-2247. [5] Gibson, L.H. and Coughlin, R.W. (2002) Optimization of high molecular weight pullulan production by Aureo-basidium pullulans in batch fermentations. Biotechnology Progress, 18, 675-678. doi:10.1021/bp0200043 [6] Chi, Z.M. and Zhao, S.Z. (2003) Optimization of medium and cultivation conditions for pullulan production by a new pullulan-producing yeast strain. Enzyme and Microbial Technology, 33, 206-211. doi:10.1016/S0141-0229(03)00119-4 [7] Lacroix, C., Leduy, A., Noel, G. and Choplin, L. (1985) Effect of pH on the batch fermentation of pullulan from sucrose medium. Biotechnology and Bioengineering, 27, 202-207. doi:10.1002/bit.260270216 [8] Gibbs, P.A. and Seviour, R.J. (1996) Does the agitation rate and/or oxygen saturation influence exopolysaccharide production by Aureobasidium pullulans in batch culture? Applied Microbiology and Biotechnology, 46, 503-510. doi:10.1007/s002530050851 [9] Barnett, C., Smith, A., Scanlon, B. and Israilides, C.J. (1999) Pullulan production by Aureobasidium pullulans growing on hydrolysed potato starch waste. Carbohydrate Polymers, 38, 203-209. doi:10.1016/S0144-8617(98)00092-7 [10] Goksungur, Y., Ucan, A. and Guvenc, U. (2003) Production of pullulan from beet molasses and synthetic medium by Aureobasidium pullulans. Turkish Journal of Biology, 28, 23-30. [11] West, T.P. and Strohfus, B. (1997) Effect of manganese on polysaccharide production and cellular pigmentation in the fungus Aureobasidium pullulans. World Journal of Microbiology and Biotechnology, 13, 233-235. doi:10.1023/A:1018554201153 [12] Youssef, F., Roukas, T. and Biliaderis, C.G. (1999) Pullulan production by a nonpigmented strain of Aureobasidium pullulans using batch and fed-batch culture. Process Biochemistry, 34, 355-366. doi:10.1016/S0032-9592(98)00106-X [13] Audet, J., Gagnon, H., Lounes, M. and Thibault, J. (1998) Polysaccharide production: Experimental comparison of the performance of four mixing devices. Bioprocess and Biosystems Engineering, 19, 45-52. doi:10.1007/s004490050481 [14] Lazaridou, A. (2002) Characterization of pullulan produced form beet molasses by Aureobasidium pullulans in a stirred rank reactor under varying agitation. Enzyme and Microbial Technology, 31, 122-132. doi:10.1016/S0141-0229(02)00082-0
[1] Leathers, T.D. (2003) Biotechnological production and applications of pullulan. Applied Microbiology and Bio-technology, 62, 468-473. doi:10.1007/s00253-003-1386-4 [2] Lee, J.H., Kim, J.H., Zhu, I.H., Zhan, X.B., Lee, J.W., Shin, D.H., et al. (2001) Optimization of conditions for the production of pullulan and high molecular weight pullulan by Aureobasidium pullulans. Biotechnology Letters, 23, 817-820. doi:10.1023/A:1010365706691 [3] Shingel, K.I. (2004) Current knowledge on biosynthesis, biological activity, and chemical modification of the exopolysaccharide, pullulan. Carbohydrate Research, 339, 447-460. doi:10.1016/j.carres.2003.10.034 [4] Thibault, J. and LeDuy, A. (1999) Pullulan, microbial production methods. In: Flickinger, M.C. and Drew, S.W., Eds., Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation, John Wiley & Sons, New York, 2232-2247. [5] Gibson, L.H. and Coughlin, R.W. (2002) Optimization of high molecular weight pullulan production by Aureo-basidium pullulans in batch fermentations. Biotechnology Progress, 18, 675-678. doi:10.1021/bp0200043 [6] Chi, Z.M. and Zhao, S.Z. (2003) Optimization of medium and cultivation conditions for pullulan production by a new pullulan-producing yeast strain. Enzyme and Microbial Technology, 33, 206-211. doi:10.1016/S0141-0229(03)00119-4 [7] Lacroix, C., Leduy, A., Noel, G. and Choplin, L. (1985) Effect of pH on the batch fermentation of pullulan from sucrose medium. Biotechnology and Bioengineering, 27, 202-207. doi:10.1002/bit.260270216 [8] Gibbs, P.A. and Seviour, R.J. (1996) Does the agitation rate and/or oxygen saturation influence exopolysaccharide production by Aureobasidium pullulans in batch culture? Applied Microbiology and Biotechnology, 46, 503-510. doi:10.1007/s002530050851 [9] Barnett, C., Smith, A., Scanlon, B. and Israilides, C.J. (1999) Pullulan production by Aureobasidium pullulans growing on hydrolysed potato starch waste. Carbohydrate Polymers, 38, 203-209. doi:10.1016/S0144-8617(98)00092-7 [10] Goksungur, Y., Ucan, A. and Guvenc, U. (2003) Production of pullulan from beet molasses and synthetic medium by Aureobasidium pullulans. Turkish Journal of Biology, 28, 23-30. [11] West, T.P. and Strohfus, B. (1997) Effect of manganese on polysaccharide production and cellular pigmentation in the fungus Aureobasidium pullulans. World Journal of Microbiology and Biotechnology, 13, 233-235. doi:10.1023/A:1018554201153 [12] Youssef, F., Roukas, T. and Biliaderis, C.G. (1999) Pullulan production by a nonpigmented strain of Aureobasidium pullulans using batch and fed-batch culture. Process Biochemistry, 34, 355-366. doi:10.1016/S0032-9592(98)00106-X [13] Audet, J., Gagnon, H., Lounes, M. and Thibault, J. (1998) Polysaccharide production: Experimental comparison of the performance of four mixing devices. Bioprocess and Biosystems Engineering, 19, 45-52. doi:10.1007/s004490050481 [14] Lazaridou, A. (2002) Characterization of pullulan produced form beet molasses by Aureobasidium pullulans in a stirred rank reactor under varying agitation. Enzyme and Microbial Technology, 31, 122-132. doi:10.1016/S0141-0229(02)00082-0