AJAC  Vol.3 No.4 , April 2012
A 100% Water Mobile Phase HPLC-PDA Analysis of Meamine and Related Analogues
Abstract: This paper describes a reserved-phase high performance liquid chromatographic method for detecting melamine (MEL) and related analogues, cyanuric acid (CYA), ammeline (AML), and ammelide (AMD), using a 100% water mobile phase. Chromatographic separation was performed an Inertsil? ODS-4 (250 × 4.6 mm, 5 μm) with a water mobile phase and a photodiode-array detector. The monitoring wavelength was adjusted to 210 nm which represents an average maximum for all the analytes. The total run time was < 8 min. The method shows high stability, significant linearity and satisfactory sensitivity. The detection limits were established in the range 23 - 46 ng.mL–1. An inexpensive and harmless method for the simultaneous detection of MEL, CYA, AML, and AMD was developed and may be further applied to the quantification in foods.
Cite this paper: N. Furusawa, "A 100% Water Mobile Phase HPLC-PDA Analysis of Meamine and Related Analogues," American Journal of Analytical Chemistry, Vol. 3 No. 4, 2012, pp. 295-299. doi: 10.4236/ajac.2012.34040.

[1]   Health Canada, “Melamine,” 2011.

[2]   World Health Organization, “Melanie and Cyanuric Acid: Toxicity, Preliminary Risk Assessment and Guidance on Levels in Food,” Updated 30 October 2008.

[3]   W. H. Tolleson, et al., “Background Paper on the Chemistry of Melamine Alone and in Combination with Related Compounds,” Health Canada, Ottawa, 2008.

[4]   E. Braekevelt, et al., “Determination of Melamine, Ammeline, Ammelide and Cyanuric Acid in Infant Formula purchased in Canada by Liquid Chromatography-Tandem Mass Spectrometry,” Food Additives and Contaminants, Vol. 28, No. 6, 2011, pp. 698-704. doi:10.1080/19440049.2010.545442

[5]   US Food and Drug Administration, “Interim Melamine and Analogues Safety/Assessment Executive Summary,” 2011. Re-viewofScientificInformationandAssessments/ucm155012. htm

[6]   G. Venkatasami and J. R. Sowa Jr., “A Rapid, Ace-tonitrile-Free, HPLC Method for Determination of Melamine in Infant Formula”, Analytica Chimica Acta, Vol. 665, No. 1, 2010, pp.227-230. doi:10.1016/j.aca.2010.03.037

[7]   M. Rambla-Alegre, et al., “Development of an Analytical Metho-dology to Quantify Melamine in Milk Using Micellarliquid Chromatography and Validation According to EU Regulation 2002/654/EC,” Talanta, Vol. 81, No. 3, 2010, pp. 894-900. doi:10.1016/j.talanta.2010.01.034

[8]   J. Mi, et al., “Agilent Technologies Application Note 5989- 9950EN,” 2008.

[9]   B. N. Tran, et al., “Use of Methanol for Efficient Extraction and Analysis of Melamine and Cyanuric Acid Residues in Dairy Products and Pet Foods”, Journal of Agricultural Food Chemistry, Vol. 58, No. 1, 2010, pp. 101- 107. doi:10.1021/jf903040z

[10]   H. Yu, et al., “Development of a High Performance Liquid Chromatography Method and a Liquid Chromatography-Tandem Mass Spectrometry Method with Pressurized Liquid Extraction for Simultaneous Quantification and Confirmation of Cyromazine, Melamine and Its Metabolites in Foods of Animal Origin,” Analytica Chimica Acta, Vol. 682, No. 1-2, 2010, pp. 48-58. doi:10.1016/j.aca.2010.09.032

[11]   S. Tittlemier, “Background Paper on Methods for the Analysis of Melamine and Related Compounds in Foods and Animal Feeds,” World Health Organization, Ottawa, 2008.

[12]   US FDA Laboratory Information Bulletin No 4421.

[13]   P. T. Anastas and J. C. Warner, “Green Chemistry: Theory and Practice,” Oxford University Press, Oxford, 1998.

[14]   T. Yoshimura, T. Nishinomiya, Y. Homda and M. Murabayashi, “Green Chemistry—Aim for the Zero Emission-Chemicals,” Sankyo Publishing Co. Ltd. Press, Tokyo, 2001.

[15]   US Department of Health and Human Services Food and Drug Administration, Center for Drug Evaluation and Research, and Center for Veterinary Medicine, US Food and Drug Administration, “Guidance for Industry: Bioanalytical Mthod Validation,” 2001. c-eRegulatoryInformation/Guidances/ucm070107.pdf#search ='FDA