Back
 CM  Vol.3 No.2 , June 2012
“Qi-Invigorating” Chinese Tonic Herbs (Shens) Stimulate Mitochondrial ATP Generation Capacity in H9c2 Cardiomyocytes in Situ and Rat Hearts ex Vivo
Abstract: In the study, using ethanol extracts of Renshen (Panax ginseng C A Meyer), Xiyangshen (Panax quinquefolius L.) and Dangshen (Codonopsis pilosulae), we investigated the effect of these “Qi-invigorating” Chinese tonic herbs on mitochondrial ATP generation capacity (ATP-GC) in H9c2 cardiomyocytes in situ and rat hearts ex vivo. All three types of “Shens” stimulated mitochondrial ATP-GC, with Renshen being most potent. While a parallel enhancement in mitochondrial ATP-GC was observed in Renshen- and Xiyangshen-pretreated rats, Dangshen treatment did not produce detectable effect ex vivo. The discrepancy between in situ and ex vivo assays for Dangshen may be attributed by its limited oral-bioavailability to the heart. The tissue specific activity of Shens on mitochondrial ATP-GC may be explained by the “Meridian Theory” in traditional Chinese medicine.
Cite this paper: H. Wong, W. Cheung, W. Tang and K. Ko, "“Qi-Invigorating” Chinese Tonic Herbs (Shens) Stimulate Mitochondrial ATP Generation Capacity in H9c2 Cardiomyocytes in Situ and Rat Hearts ex Vivo," Chinese Medicine, Vol. 3 No. 2, 2012, pp. 101-105. doi: 10.4236/cm.2012.32016.
References

[1]   D. D. Kitts and C. Hu, “Efficacy and Safety of Ginseng,” Public Health Nutrition, Vol. 3, No. 4a, 2000, pp. 473-485. doi:10.1017/S1368980000000550

[2]   J.-M. Lu, Q. Z. Yao and C. Y. Chen, “Ginseng Compounds: An Update on Their Molecular Mechanisms and Medical Applications,” Current Vascular Pharmacology, Vol. 7, No. 3, 2009, pp. 293-302. doi:10.2174/157016109788340767

[3]   L.-W. Qi, C.-Z. Wang and C.-S. Yuan, “American Ginseng: Potential Structure-Function Relationship in Cancer Chemoprevention,” Biochemical Pharmacology, Vol. 80, No. 7, 2010, pp. 947-954. doi:10.1016/j.bcp.2010.06.023

[4]   T. K. Yun, “Panax Ginseng—A Non-Organ-Specific Cancer Preventive?” Lancet Oncology, Vol. 2, No. 1, 2001, pp. 49-55. doi:10.1016/S1470-2045(00)00196-0

[5]   S. F. Chu and J. T. Zhang, “New Achievements in Ginseng Research and Its Future Prospects,” Chinese Journal of Integrative Medicine, Vol. 15, No. 6, 2009, pp. 403-408. doi:10.1007/s11655-009-0403-6

[6]   W. C. S. Cho, W.-S. Chung, S. K. W. Lee, A. W. N. Leung, C. H. K. Cheng and K. K. M. Yue, “Ginsenoside Re of Panax Ginseng Possesses Significant Antioxidant and Antihyperlipidemic Efficacies in Streptozotocin-Induced Diabetic Rats,” European Journal of Pharmacology, Vol. 550, No. 1-3, 2006, pp. 173-179. doi:10.1016/j.ejphar.2006.08.056

[7]   X. T. Li, J. J. Zhang and W. W. Chen, “The Effects of TCM Qi-Invigorating, Qi Regulating Drugs and Polysaccharides on the Energy Charge of Rat Skeletal Muscle Cells,” Journal of Beijing University of Traditional Chinese Medicine, Vol. 23, No. 5, 2000, pp. 36-38.

[8]   H. Y. Leung and K. M. Ko, “Herba Cistanche Extract Enhances Mitochondrial ATP Generation in Rat Hearts and H9c2 Cells,” Pharmaceutical Biology, Vol. 46, No. 6, 2008, pp. 418-424. doi:10.1080/13880200802055883

[9]   H. S. Wong, H. Y. Leung and K. M. Ko, “Yang-Invigorating Chinese Tonic Herbs Enhance Mitochondrial ATP Generation in H9c2 Cardiomyocytes,” Chinese Medicine, Vol. 2, No. 1, 2011, pp. 1-5. doi:10.4236/cm.2011.21001

[10]   H. Y. Leung, P. Y. Chiu, M. K. T. Poon and K. M. Ko, “A Yang-Invigorating Chinese Herbal Formula Enhances Mitochondrial Functional Ability and Antioxidant Capacity in Various Tissues of Male and Female Rats,” Rejuvenation Research, Vol. 8, No. 4, 2005, pp. 238-247. doi:10.1089/rej.2005.8.238

[11]   P. Ronner, E. Friel, K. Czerniawski and S. Frankle, “Luminometric Assays of ATP, Phosphocreatine, and Creatine for Estimation of Free ADP and Free AMP,” Analytical Biochemistry, Vol. 275, No. 2, 1999, pp. 208-216. doi:10.1006/abio.1999.4317

[12]   Z. C. Lin, J. Y. Zhang, Y. R. Ke, Z. C. Chen and Z. L. Guo, “A Pentsaological Research on Dangshen (Codonopsis SPP., Campanulaceae),” Journal of Chinese Medicine, Vol. 18, No. 1-2, 2007, pp. 51-64. (in Chinese)

[13]   H. J. Jiao, “The Pharmacological Basis and Clinical Applications of Dangshen,” Clinical Medicine, Vol. 25, No. 4, 2005. (in Chinese)

[14]   Y. X. Sun and J. C. Liu, “Structural Characterization of A Water-Soluble Polysaccharide from the Roots of Codonopsis Pilosula and Its Immunity Activity,” International Journal of Biological Macromolecules, Vol. 43, No. 3, 2008, pp. 279-282. doi:10.1016/j.ijbiomac.2008.06.009

[15]   Y. Shen, “Protective Effect of Spleen_Invigorating Chinese Medicinal Herbs for Gastric Mucosa,” World Chinese Journal of Digestology, Vol. 4, No. 7, 1999, pp. 357-358.

 
 
Top