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 OJGen  Vol.2 No.1 , March 2012
Irr1/Scc3 cohesin interacts with Rec8 in meiotic prophase of Saccharomyces cerevisiae
Agata Cena, Piotr Kowalec, Danuta Płochocka, Anna Kurlandzka*
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Abstract: The meiotic cohesin complex of S. cerevisiae shares with the mitotic one the Irr1/Scc3, Smc1, and Smc3 subunits, while the meiosis-specific subunit Rec8 replaces mitotic subunit Scc1/Mcd1. We noticed earlier that the irr1-1 mutation (F658G) severely affected meiosis. The irr1 1/IRR1 cells were entering meiosis before having completed mitotic cell division. Using meiotic two-hybrid assay and co-immunoprecipitation we show that in cells arrested in pachytene due to a lack of a gene-regulatory factor Ndt80, the Irr1 protein interacts with Rec8p and the irr1-1 mutation abolishes this interaction. These findings indicate an important role of Irr1p in early stages of meiosis.
Keywords: Irr1/Scc3; Cohesin; Rec8; Meiosis; Saccharomyces cerevisiae
Cite this paper: Cena, A. , Kowalec, P. , Płochocka, D. and Kurlandzka, A. (2012) Irr1/Scc3 cohesin interacts with Rec8 in meiotic prophase of Saccharomyces cerevisiae. Open Journal of Genetics, 2, 1-6. doi: 10.4236/ojgen.2012.21001.
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