Irr1/Scc3 cohesin interacts with Rec8 in meiotic prophase of Saccharomyces cerevisiae
Affiliation(s)
Institute of Biochemistry and Biophysics, Polish Academy of Sciences Pawinskiego 5A 02-106 Warszawa, Poland.
Institute of Biochemistry and Biophysics, Polish Academy of Sciences Pawinskiego 5A 02-106 Warszawa, Poland.
ABSTRACT
The meiotic cohesin complex of S. cerevisiae shares with the mitotic one the Irr1/Scc3, Smc1, and Smc3 subunits, while the meiosis-specific subunit Rec8 replaces mitotic subunit Scc1/Mcd1. We noticed earlier that the irr1-1 mutation (F658G) severely affected meiosis. The irr1 1/IRR1 cells were entering meiosis before having completed mitotic cell division. Using meiotic two-hybrid assay and co-immunoprecipitation we show that in cells arrested in pachytene due to a lack of a gene-regulatory factor Ndt80, the Irr1 protein interacts with Rec8p and the irr1-1 mutation abolishes this interaction. These findings indicate an important role of Irr1p in early stages of meiosis.
The meiotic cohesin complex of S. cerevisiae shares with the mitotic one the Irr1/Scc3, Smc1, and Smc3 subunits, while the meiosis-specific subunit Rec8 replaces mitotic subunit Scc1/Mcd1. We noticed earlier that the irr1-1 mutation (F658G) severely affected meiosis. The irr1 1/IRR1 cells were entering meiosis before having completed mitotic cell division. Using meiotic two-hybrid assay and co-immunoprecipitation we show that in cells arrested in pachytene due to a lack of a gene-regulatory factor Ndt80, the Irr1 protein interacts with Rec8p and the irr1-1 mutation abolishes this interaction. These findings indicate an important role of Irr1p in early stages of meiosis.
Cite this paper
Cena, A. , Kowalec, P. , Płochocka, D. and Kurlandzka, A. (2012) Irr1/Scc3 cohesin interacts with Rec8 in meiotic prophase of Saccharomyces cerevisiae. Open Journal of Genetics, 2, 1-6. doi: 10.4236/ojgen.2012.21001.
Cena, A. , Kowalec, P. , Płochocka, D. and Kurlandzka, A. (2012) Irr1/Scc3 cohesin interacts with Rec8 in meiotic prophase of Saccharomyces cerevisiae. Open Journal of Genetics, 2, 1-6. doi: 10.4236/ojgen.2012.21001.
References
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[1] Marston, A.L. and Amon, A. (2004) Meiosis: Cell-cycle controls shuffle and deal. Nature Reviews Molecular Cell Biology, 5, 983-997. doi:10.1038/nrm1526 [2] Lee, B. and Amon, A. (2001) Meiosis: How to create a specialized cell cycle. Current Opinion in Cell Biology, 13, 770-777. doi:10.1016/S0955-0674(00)00282-9 [3] Brar, G.A., Hochwagen, A. and Ee, L.S. et al. (2009) The multiple roles of cohesin in meiotic chromosome mor- phogenesis and pairing. Molecular Biology of the Cell, 20, 1030-1047. doi:10.1091/mbc.E08-06-0637 [4] Klein, F., Mahr, P., Galova, M., et al. (1999) A central role for cohesins in sister chromatid cohesion, formation of axial elements, and recombination during yeast meiosis. Cell, 98, 91-103. doi:10.1016/S0092-8674(00)80609-1 [5] Nasmyth, K. (2001) Disseminating the genome: joining, resolving, and separating sister chromatids during mitosis and meiosis. Annual Review of Genetics, 35, 673-745. doi:10.1146/annurev.genet.35.102401.091334 [6] Buonomo, S.B., Clyne, R.K., Fuchs, J., et al. (2000) Disjunction of homologous chromosomes in meiosis I depends on proteolytic cleavage of the meiotic cohesin Rec8 by separin. Cell, 103, 387-398. doi:10.1016/S0092-8674(00)00131-8 [7] Shonn, M.A., McCarroll, R. and Murray, A.W. (2000) Requirement of the spindle checkpoint for proper chromosome segregation in budding yeast meiosis. Science, 289, 300-303. doi:10.1126/science.289.5477.300 [8] Uhlmann, F. (2003) Separase regulation during mitosis. Biochemical Society Symposia, 70, 243-251. [9] Katis, V.L., Lipp, J.J., Imre, R., et al. (2010) Rec8 phosphorylation by casein kinase 1 and Cdc7-Dbf4 kinase regulates cohesin cleavage by separase during meiosis. Developmental Cell, 18, 397-409. doi:10.1016/j.devcel.2010.01.014 [10] Brar, G.A., Kiburz, B.M., Zhang, Y., et al. (2006) Rec8 phosphorylation and recombination promote the step-wise loss of cohesin in meiosis. Nature, 441, 532-536. doi:10.1038/nature04794 [11] Cena A., Koz?owska E., P?ochocka D., et al. (2008) The F658G substitution in Saccharomyces cerevisiae cohesin Irr1/Scc3 is semidominant in the diploid and disturbs mitosis, meiosis and the cell cycle. European Journal of Cell Biology, 87, 8318-8344. doi:10.1016/j.ejcb.2008.05.002 [12] Chu, S. and Herskowitz, I. (1998) Gametogenesis in yeast is regulated by a transcriptional cascade dependent on Ndt80. Molecular Cell, 1, 685-696. doi:10.1016/S1097-2765(00)80068-4 [13] Rose, M., Winston, F. and Hieter, P. (1990) Methods in yeast genetics. A cold spring harbor laboratory course. Cold Spring Harbor Laboratory Press, New York. [14] Arora, C., Kee, K., Maleki, S., et al. (2004) Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism. Molecular Cell, 13, 549-559. doi:10.1016/S1097-2765(04)00063-2 [15] Buonomo, S.B., Rabitsch, K.P., Fuchs, J., et al. (2003) Division of the nucleolus and its release of CDC14 during anaphase of meiosis I depends on separase, SPO12, and SLK19. Developmental Cell, 4, 727-739. doi:10.1016/S1534-5807(03)00129-1 [16] Gruber, S., Haering, C.H. and Nasmyth, K. (2003) Chromosomal cohesin forms a ring. Cell, 112, 765-777. doi:10.1016/S0092-8674(03)00162-4 [17] Bialkowska, A. and Kurlandzka, A. (2002) Proteins interacting with Lin 1p, a putative link between chromosome segregation, mRNA splicing and DNA replication in Saccharomyces cerevisiae. Yeast, 19, 1323-1333. doi:10.1002/yea.919 [18] Hunter, S., Apweiler, R., Attwood, T.K., et al. (2009) InterPro: The integrative protein signature database. Nucleic Acids Research, 37, D224-D228. doi:10.1093/nar/gkn785 [19] Finn, R.D., Mistry, J., Tate, J., et al. (2010) The Pfam protein families database. Nucleic Acids Research, 38, D211-D222. doi:10.1093/nar/gkp985