Irr1/Scc3 cohesin interacts with Rec8 in meiotic prophase of Saccharomyces cerevisiae

Agata  Cena; Piotr  Kowalec; Danuta  Płochocka; Anna  Kurlandzka

doi:10.4236/ojgen.2012.21001

Agata Cena, Piotr Kowalec, Danuta Płochocka, Anna Kurlandzka^*

Abstract: The meiotic cohesin complex of S. cerevisiae shares with the mitotic one the Irr1/Scc3, Smc1, and Smc3 subunits, while the meiosis-specific subunit Rec8 replaces mitotic subunit Scc1/Mcd1. We noticed earlier that the irr1-1 mutation (F658G) severely affected meiosis. The irr1 1/IRR1 cells were entering meiosis before having completed mitotic cell division. Using meiotic two-hybrid assay and co-immunoprecipitation we show that in cells arrested in pachytene due to a lack of a gene-regulatory factor Ndt80, the Irr1 protein interacts with Rec8p and the irr1-1 mutation abolishes this interaction. These findings indicate an important role of Irr1p in early stages of meiosis.

Keywords: Irr1/Scc3; Cohesin; Rec8; Meiosis; Saccharomyces cerevisiae

Cite this paper: Cena, A. , Kowalec, P. , Płochocka, D. and Kurlandzka, A. (2012) Irr1/Scc3 cohesin interacts with Rec8 in meiotic prophase of Saccharomyces cerevisiae. Open Journal of Genetics, 2, 1-6. doi: 10.4236/ojgen.2012.21001.

References

[1] Marston, A.L. and Amon, A. (2004) Meiosis: Cell-cycle controls shuffle and deal. Nature Reviews Molecular Cell Biology, 5, 983-997. doi:10.1038/nrm1526

[2] Lee, B. and Amon, A. (2001) Meiosis: How to create a specialized cell cycle. Current Opinion in Cell Biology, 13, 770-777. doi:10.1016/S0955-0674(00)00282-9

[3] Brar, G.A., Hochwagen, A. and Ee, L.S. et al. (2009) The multiple roles of cohesin in meiotic chromosome mor- phogenesis and pairing. Molecular Biology of the Cell, 20, 1030-1047. doi:10.1091/mbc.E08-06-0637

[4] Klein, F., Mahr, P., Galova, M., et al. (1999) A central role for cohesins in sister chromatid cohesion, formation of axial elements, and recombination during yeast meiosis. Cell, 98, 91-103. doi:10.1016/S0092-8674(00)80609-1

[5] Nasmyth, K. (2001) Disseminating the genome: joining, resolving, and separating sister chromatids during mitosis and meiosis. Annual Review of Genetics, 35, 673-745. doi:10.1146/annurev.genet.35.102401.091334

[6] Buonomo, S.B., Clyne, R.K., Fuchs, J., et al. (2000) Disjunction of homologous chromosomes in meiosis I depends on proteolytic cleavage of the meiotic cohesin Rec8 by separin. Cell, 103, 387-398. doi:10.1016/S0092-8674(00)00131-8

[7] Shonn, M.A., McCarroll, R. and Murray, A.W. (2000) Requirement of the spindle checkpoint for proper chromosome segregation in budding yeast meiosis. Science, 289, 300-303. doi:10.1126/science.289.5477.300

[8] Uhlmann, F. (2003) Separase regulation during mitosis. Biochemical Society Symposia, 70, 243-251.

[9] Katis, V.L., Lipp, J.J., Imre, R., et al. (2010) Rec8 phosphorylation by casein kinase 1 and Cdc7-Dbf4 kinase regulates cohesin cleavage by separase during meiosis. Developmental Cell, 18, 397-409. doi:10.1016/j.devcel.2010.01.014

[10] Brar, G.A., Kiburz, B.M., Zhang, Y., et al. (2006) Rec8 phosphorylation and recombination promote the step-wise loss of cohesin in meiosis. Nature, 441, 532-536. doi:10.1038/nature04794

[11] Cena A., Koz?owska E., P?ochocka D., et al. (2008) The F658G substitution in Saccharomyces cerevisiae cohesin Irr1/Scc3 is semidominant in the diploid and disturbs mitosis, meiosis and the cell cycle. European Journal of Cell Biology, 87, 8318-8344. doi:10.1016/j.ejcb.2008.05.002

[12] Chu, S. and Herskowitz, I. (1998) Gametogenesis in yeast is regulated by a transcriptional cascade dependent on Ndt80. Molecular Cell, 1, 685-696. doi:10.1016/S1097-2765(00)80068-4

[13] Rose, M., Winston, F. and Hieter, P. (1990) Methods in yeast genetics. A cold spring harbor laboratory course. Cold Spring Harbor Laboratory Press, New York.

[14] Arora, C., Kee, K., Maleki, S., et al. (2004) Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism. Molecular Cell, 13, 549-559. doi:10.1016/S1097-2765(04)00063-2

[15] Buonomo, S.B., Rabitsch, K.P., Fuchs, J., et al. (2003) Division of the nucleolus and its release of CDC14 during anaphase of meiosis I depends on separase, SPO12, and SLK19. Developmental Cell, 4, 727-739. doi:10.1016/S1534-5807(03)00129-1

[16] Gruber, S., Haering, C.H. and Nasmyth, K. (2003) Chromosomal cohesin forms a ring. Cell, 112, 765-777. doi:10.1016/S0092-8674(03)00162-4

[17] Bialkowska, A. and Kurlandzka, A. (2002) Proteins interacting with Lin 1p, a putative link between chromosome segregation, mRNA splicing and DNA replication in Saccharomyces cerevisiae. Yeast, 19, 1323-1333. doi:10.1002/yea.919

[18] Hunter, S., Apweiler, R., Attwood, T.K., et al. (2009) InterPro: The integrative protein signature database. Nucleic Acids Research, 37, D224-D228. doi:10.1093/nar/gkn785

[19] Finn, R.D., Mistry, J., Tate, J., et al. (2010) The Pfam protein families database. Nucleic Acids Research, 38, D211-D222. doi:10.1093/nar/gkp985