OJBD  Vol.2 No.1 , March 2012
Leukemogenic Fusion Gene (p190 BCR-ABL) Transduction into Hematopoietic Stem/Progenitor Cells in the Common Marmoset
Abstract: Patients with Philadelphia chromosome (p190 BCR-ABL fusion gene)-positive acute lymphoblastic leukemia have a poor prognosis despite intensive therapeutic intervention.In this study, we attempted to develop a leukemia nonhuman primate model that mimics various human systems. Hematopoietic stem/progenitor cells in the common marmoset were transduced with a lentiviral vector containing the p190 BCR-ABL fusion gene by ex vivo transduction or in vivo direct bone marrow injection. In the latter model, BCR-ABL gene expression was maintained for more than one and a half years. One marmoset unexpectedly developed myelofibrosis-like disease. However, none of the marmosets have developed leukemia to date. In conclusion, we successfully achieved sustained p190 BCR-ABL gene expression in vivo. However, a genetic mutation in addition to p190 BCR-ABL may be required for the malignant transformation of hematopoietic stem/progenitor cells in the common marmoset during the short observation period. This novel in vivo approach will help develop a marmoset leukemia model in the future.
Cite this paper: Y. Dong, S. Kobayashi, Y. Tian, M. Ozawa, T. Hiramoto, K. Izawa, Y. Bai, Y. Soda, E. Sasaki, T. Itoh, Y. Maru, S. Takahashi, K. Uchimaru, N. Oyaizu, A. Tojo, C. Kai and K. Tani, "Leukemogenic Fusion Gene (p190 BCR-ABL) Transduction into Hematopoietic Stem/Progenitor Cells in the Common Marmoset," Open Journal of Blood Diseases, Vol. 2 No. 1, 2012, pp. 1-10. doi: 10.4236/ojbd.2012.21001.

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