Health  Vol.2 No.5 , May 2010
The effect of detergent as polluting agent on the photosynthetic activity and chlorophyll content in bean leaves
Abstract: The paper investigates effects of detergent for domestic use on the photosynthetic activity and chlorophyll content in intact bean leaves. The plants were watered for 21 days with a solution of domestic washing powder of 0.60 g r/l. It was established that the activity of photosynthetic apparatus in the plant leaf PhACNorm [%] decreases exponentially with the length of plant treatment/watering. At the end of the treatment (21st day) the activity of photosynthetic apparatus in the dosed plant leaf was no more than 45% of that in control plant (those which were not watered with detergent solution). With increased plant treatment duration the changed chlorophyll concentration ΔChlNorm [%] rose non-linearly in plant leaves. The highest change ΔChlNorm [%] was observed on the 21st day and amounted to 12%.
Cite this paper: nullJovanic, B. , Bojovic, S. , Panic, B. , Radenkovic, B. and Despotovic, M. (2010) The effect of detergent as polluting agent on the photosynthetic activity and chlorophyll content in bean leaves. Health, 2, 395-399. doi: 10.4236/health.2010.25059.

[1]   Imandel, K., Razeghi, N. and Samar, P. (1978) Tehran ground water pollution by detergent. Water, Air, & Soil Pollution, 9, 119-122.

[2]   Korshenko, A. and GasimGul, A. (2005) Pollution of the Caspian Sea. Handbook of Environmental Chemistry, Springer-Verlag Berlin Heidelberg, 5, Part P, 109-142.

[3]   Adekola, B.N. and Eletta, O.A.A. (2007) A study of heavy metal pollution of Asa River, Ilorin. Nigeria; trace metal monitoring and geochemistry. Environmental Monitoring and Assessment, 12, 157-163.

[4]   Zhuravel, V.E., Bezverbnaya, I.P. and Buzoleva, S.L. (2004) Microbian indication of pollution of the coastal zone of the sea of Okhotsk and Avacha Bay. Russian Journal of Marine Biology, 30(2), 121-126.

[5]   Burdon, F., Bouchaud, J.P., Tannoudji, A. and Levy, C.C. (2002) Statistics & Laser Cooling (Paperback), Cambridge University Press, UK.

[6]   Lichthenthaler, K.H. and Buschmann, C. (1987) Chlorophyll Fluorescence Spectra of Green Bean Leaves. Journal of Plant Physiology, 129(1-2), 137-147.

[7]   Lichtenthaler, K.H. and Riderle, U. (1988) The role of the chlorophyll fluorescence in the detection of stress conditions in plants. CRC Critical Reviews in Analytical Chemistry, 19, S29-S85.

[8]   Jovanić, R.B. and Dramićanin, D.M. (2003) In vivo monitoring of chlorophyll fluorescence response to low-dose γ-irradiation in Pumpkin (Cucurbita pepo). Luminescence, 18, 274-277.

[9]   Aizdaicher, N.A. and Reunova, Yu. A. (2002) Effects of detergents on in vitro growth of diatom alga thalassiosira pseudonana. Russian Journal of Marine Biology, 28(5), 324-328.

[10]   Vasconcelos, A., Silva, C.J.S.M., Schroeder, M., Guebitz, G.M. and Cavaco-Paulo, A. (2006) Detergent formulations for wool domestic washings containing immobilized enzymes. Biotechnology Letters, 28(10), 725-731.

[11]   Ca´rdenas, L., Vidali, L., Domı´nguez, J., Pe´rez, H., Sa´nchez, F., Hepler, K.P. and Carmen Quinto, C. (1998) Rearrangement of actin microfilaments in plant root hairs responding to rhizobium etli nodulation signals. Plant Physiology, 116(3), 871-877.

[12]   Nand, L. and Richa, M. (2003) Synthetic detergent induced changes in the seed inhibition pattern and dehydrogenese activity in mungbean (Vigna radiata). EcoEnvConserv, 9(3), 379-383.

[13]   Park, J., Gu, Y., Lee, Y., Yang, Z. and Lee, Y. (2004) Phosphatidic acid induces leaf cell death in arabidopsis by activating the rho-related small G protein GTPase- mediated pathway of reactive oxygen species generation. Plant Physiology, 134(1), 129-136.

[14]   Behzadipou, M., Kluge, M. and Liithjea, S. (2001) Changes in plasma membrane fluidity of corn (Zea mays L.) roots after Brij 58 treatment. Protoplasma, 217, 65- 69.

[15]   Brandt, K.K., Hesseloy, M.E., Rosloev, E.P., Enriksen, K. and Oyrensen, J.S. (2001) Toxic effects of linear alkylbenzene sulfonate on metabolic activity, growth rate, and microcolony formation of nitrosomonas and nitrosospira strains. Applied and Environmental Microbiology, 67(6), 2489-2498.

[16]   Nanba, O. and Satoh, K. (1987), Proceedings of the National Academy of Sciences, USA. 84, 109-112.

[17]   Reunova, Y.A. and Ayzdaycher, N.A. (2003) Effects of detergent on chlorophyll a content and quantity dynamics of microalga Chroomonas salina (Wils.) Butch. (Cryptophyta). International Journal on Algae, 5, 106-110.

[18]   Mimuro, M. and Katoh, T. (1991) Carotenoids in photosynthesis: Absorption, transfer and dissipation of light energy. Pure and Applied Chemistry, 63(1), 123-130.

[19]   Green, B.R. (1988) The chlorophyll-protein complexes of higher plant photosynthetic membranes or Just what green band is that? Photosynthesis Research, 15(1), 30- 32.

[20]   Gadallah, M.A.A. (2004) Phytotoxic effects of industrial and sewage waste waters on growth, chlorophyll content, transpiration rate and relative water content of potted sunflower plants. Water, Air, & Soil Pollution, 89(1-2), 33-47.

[21]   Szabad, J., Lehoczki, E., Szalay, L. and Csatorday, K. (1984) Lutein-chlorophyll-a energy transfer in detergent micelles, Biophysics of Structure & Mechanism, 1(1), 65- 74.

[22]   Eggink, L.L., Park, H. and Hoober, J.K. (2001) The role of chlorophyll b in photosynthesis: Hypothesis, BioMed Central.

[23]   Murphy, D.J. and Woodrow, I.E. (1984) The effects of Triton X-100 and n-octyl f-D-glucopyranoside on energy transfer in photosynthetic membranes. Biochemical Journal, 224(3), 989-993.

[24]   Liu, S., Dong, F.Q., Tang, C.Q., Kuang, T.Y., Li, L.B. and Liu, Y. (2006) Photodamage to pigment in the photosystem reaction center D1/D2/Cytochrome b559 complex. Journal of Integrated Plant Biology, 48(7), 800- 806.

[25]   Moya, I., Silvestri, M., Vallon, O., Cinque, G. and Bassi, R. (2001) Time-resolved fluorescence analysis of the photosystem II antenna proteins in detergent micelles and liposomes. American Chemical Society.

[26]   Tang, D., Jankowiak, R., Seibert, M. and Small, G.J. (1991) Effects of detergent on the excited state structure and relaxation dynamics of the photosystem II reaction center: A high resolution hole burning study. Journal of Photosynthesis Research, 27(1), 19-29.

[27]   Ivanov, B.N., Ignatova, L.K. and Romanova, A.K. (2007) Diversity in forms and functions of carbonic anhydrase in terrestrial higher plants. Russian Journal of Plant Physiology, 54(2), 143-162.

[28]   Santacruz-Ruvalcaba, F., Gutiérrez-Pulido, H. and Rodríguez-Garay, B. (1999) Efficient in vitro propagation of agave parrasana berger. Plant Cell, Tissue and Organ Culture, 56(3), 163-167.

[29]   Klimov, V.V., Karapetian, N.V. and Krasnovskiĭ, A.A. (1975) The effect of detergent Triton X = 100 on the light induced changes in the fluorescence yield of chloroplasts. Journal of Molecular Biology (Mosk), 9, 219-226.

[30]   Katoh, S. (2003) Early research on the role of plastocyanin in photosynthesis. Photosynthesis Research, 76(1-3), 255-261.

[31]   Dekker, J.P., Germano, M., Roon, H. and Boekema, E,J. (2002) Photosystem II solubilizes as a monomer by mild detergent treatment of unstacked thylakoid membranes. Photosynthesis Research, 72(2), 203-210.

[32]   Vernon, L.P. (2003) Photosynthesis and the Charles F. Kettering Research Laboratory. Photosynthesis Research, 76(1-3), 379-388.