Health  Vol.4 No.2 , February 2012
Experimental antithrombotic effect of potatoes harvested in the autumn
Abstract: Prevention of arterial thrombotic diseases has a high priority in developed countries. While an inappropriate diet is known to enhance the risk for acute thrombotic events, a regular diet with proven antithrombotic effects might be beneficial in the prevention of such diseases. The present study is part of a series of investigations aimed to assess the possible antithrombotic activity of various fruits and vegetables. Previously we demonstrated antithrombotic effect of specific potato varieties harvested in the spring. The present study aimed to test seven varieties of potatoes, which were harvested in the autumn. The in vitro Global Thrombosis Test (GTT) was used for selection, which was then followed by the He-Ne laser-induced in vivo thrombosis test in mice treated with potato filtrates orally. We have shown that all seven potato varieties showed some antithrombotic effect and the heat-resistant effect of four varieties was highly significant. Our present findings add further components to a diet containing fruits and vegetables with antithrombotic effect.
Cite this paper: Yamamoto, J. , Masuda, M. , Hyodo, K. and Iwasaki, M. (2012) Experimental antithrombotic effect of potatoes harvested in the autumn. Health, 4, 108-112. doi: 10.4236/health.2012.42017.

[1]   Lichtenstein, A.H., Appel, L.J., Brands, M., Carnethon, M., Daniels, S., Franch, H.A., Franklin, B., Kris-Etherton, P., Harris, W.S., Howard, B., Karanja, N., Lefevre, M., Rudel, L., Sacks, F., Van Horn, L., Winston, M. and Wylie-Rosett, J. (2006) Summary of American heart association diet and lifestyle recommendations revision. Arteriosclerosis, Thrombosis and Vascular Biology, 26, 2186-2191. doi:10.1161/01.ATV.0000238352.25222.5e

[2]   Ulbricht, T.L. and Southgate, D.A. (1991) Coronary heart disease: Seven dietary factors. Lancet, 338, 985-992. doi:10.1016/0140-6736(91)91846-M

[3]   Renaud, S. and De Lorgeril, M. (1992) Wine, alcohol, platelets, and the French paradox for coronary heart disease. Lancet, 339, 1523-1526. doi:10.1016/0140-6736(92)91277-F

[4]   Joshipura, K.J., Ascherio, A., Manson, J.E., Stampfer, M.J., Rimm, E.B., Speizer, F.E., Hennekens, C.H., Spiegelman, D. and Willett, W.C. (1999) Fruit and vegetable intake in relation to risk of ischemic stroke. Journal of the American Medical Association, 282, 1233-1239. doi:10.1001/jama.282.13.1233

[5]   Liu, S., Manson, J.E., Lee, I.M., Cole, S.R., Hennekens, C.H., Willett, W.C. and Buring, J.E. (2000) Fruit and vegetable intake and risk of cardiovascular disease: The women’s health study. American Journal of Clinical Nutrition, 72, 922-928.

[6]   Joshipura, K.J., Hu, F.B., Manson, J.E., Stampfer, M.J., Rimm, E.B., Speizer, F.E., Colditz, G., Ascherio, A., Rosner, B., Spiegelman, D. and Willett, W.C. (2001) The effect of fruit and vegetable intake on risk for coronary heart disease. Annals of the Internal Medicine, 134, 1106-1114.

[7]   Bazzano, L.A., He, J., Ogden, L.G., Loria, C.M., Vupputuri, S., Myers, L. and Whelton, P.K. (2002) Fruit and vegetable intake and risk of cardiovascular disease in US adults: The first national health and nutrition examination survey epidemiologic follow-up study. American Journal of the Clinical Nutrition, 76, 93-99.

[8]   Harrison, P. (2005) Platelet function analysis. Blood Review, 19, 111-123. doi:10.1016/j.blre.2004.05.002

[9]   Ferreiro, J.L., Sibbing, D. and Angiolillo, D.J. (2010) Platelet function testing and risk of bleeding complications. Thrombosis et Haemostasis, 103, 1128-1135. doi:10.1160/TH09-11-0799

[10]   Lippi, G., Cervellin, G., Franchini, M. and Favaloro, E.J. (2010) Biochemical markers for the diagnosis of venous thromboembolism: the past, present and future. Journal of the Thrombosis and Thrombolysis, 30, 459-471. doi:10.1007/s11239-010-0460-x

[11]   Gorog, D.A., Sweeny, J.M. and Fuster, V. (2009) Antiplatelet drug “resistance”. Part 2: Laboratory resistance to antiplatelet drugs-fact or artifact? Nature Reviews Cardiology, 6, 365-373.

[12]   Ratnatunga, C.P., Edmondson, S.F., Rees, G.M. and Kovacs, I.B. (1982) High-dose aspirin inhibits shear-induced platelet reaction involving thrombin generation. Circulation, 85, 1077-1082.

[13]   Gorog, D.A. and Kovacs, I.B. (1995) Thrombotic status analyser. Thrombosis et Haemostasis, 73, 514-520.

[14]   Yamamoto, J., Naemura, A., Ura, M., Ijiri, Y., Yamashita, T., Kurioka, A. and Koyama, A. (2006) Testing various fruits for anti-thrombotic effect: I. Mulberries. Platelets, 17, 555-564. doi:10.1080/09537100600759295

[15]   Yamamoto, J., Naemura, A., Ijiri, Y., Ogawa, K., Suzuki, T., Shimada, Y., Giddings, J.C. (2008) The antithrombotic effects of carrot filtrates in rats and mice. Blood Coagulation & Fibrinolysis, 19, 785-792. doi:10.1097/MBC.0b013e3283177b19

[16]   Yamamoto, J., Yamashita, T., Ikarugi, H., Taka, T., Hashimoto, M., Ishii, H., Watanabe, S. and Kovacs, I.B. (2003) Gorog thrombosis test: A global in-vitro test of platelet function and thrombolysis. Blood Coagulation & Fibrinolysis, 14, 31-39. doi:10.1097/00001721-200301000-00007

[17]   Kovacs, I.B., Tigyi-Sebes, A., Trombitas, K. and Gorog, P. (1975) Evans blue: An ideal energy-absorbing material to produce intravascular microinjury by He-Ne gas laser. Microvascular Research, 10, 107-124. doi:10.1016/0026-2862(75)90025-4

[18]   Ijiri, Y., Miura, M., Hashimoto, M., Fukunaga, C., Wata-nabe, S., Kubota, A., Oiwa, K., Okuda, T. and Yamamoto, J. (2002) A new model to evaluate the diet-induced prothrombotic status, using He-Ne laser-induced throm-bogenesis in the carotid artery of apolipoprotein E-deficient and low-density lipoprotein receptor-deficient mice. Blood Coagulation & Fibrinolysis, 13, 497-504. doi:10.1097/00001721-200209000-00004

[19]   Yamamoto, J., Taka, T., Yamada, K., Ijiri, Y., Murakami, M., Hirata, Y., Naemura, A., Hashimoto, M., Yamashita, T., Oiwa, K., Seki, J., Suganuma, H., Inakuma, T. and Yoshida, T. (2003) Tomatoes have natural antithrombotic effects. British Journal of Nutrition, 90, 1031-1038. doi:10.1079/BJN2003994

[20]   Naemura, A., Mitani, T., Ijiri, Y., Tamura, Y., Yamashita, T., Okimura, M. and Yamamoto, J. (2005) Anti-thrombotic effect of strawberries. Blood Coagulation & Fibrinolysis, 16, 501-509. doi:10.1097/01.mbc.0000184737.50594.a8

[21]   Ichiura, D., Naemura, A., Ura, M., Mori, M. and Yamamoto, J. (2009) Antithrombotic effect of potato in animal experiments. Food, 3, 8-12.