JDM  Vol.2 No.1 , February 2012
Effect of quercetin on postprandial glucose excursion after mono- and disaccharides challenge in normal and diabetic rats
Abstract: Postprandial hyperglycemia is a major risk factor for diabetic complications leading to disabilities and mortality in diabetics. Quercetin, a flavonoid, has been tried in traditional medicine for treating diabetes. The present study was designed to evaluate the potential of quercetin to control postprandial blood glucose level after maltose and glucose loading in normal and STZ-induced diabetic rats. Normal male Albino wistar rats and STZ-induced diabetic rats were treated with 300 and 600 mg/kg quercetin orally to evaluate the effect on postprandial hyperglycemia after carbohydrate loading, using acarbose as comparator. The results clearly showed ameliorated postprandial hyperglycemia due to the use of quercetin (300 and 600 mg/kg), it significantly dampened the postprandial hyperglycemia by 32.0% and 64.0% respectively, in maltose loaded diabetic rats, and 30.3% after 300 mg/kg dose in normal rats, compared to control; while acarbose produced 51% and 54% decrease in this respect in the two models respecttively. Quercetin in 600 mg/kg dose produces significantly more reduction in postprandial hyperglycemia compared to acarbose, while in rats that received glucose and quercetin, postprandial hyperglycemia was not significantly affected. In conclusion, quercetin effectively suppresses postprandial hyperglycemia in STZ-induced diabetic rats loaded with maltose, which may be attributed to α-glucosidase inhibition. Quercetin could be used as a potential supplement for treating postprandial hyperglycemia.
Cite this paper: Hussain, S. , Ahmed, Z. , Mahwi, T. and Aziz, T. (2012) Effect of quercetin on postprandial glucose excursion after mono- and disaccharides challenge in normal and diabetic rats. Journal of Diabetes Mellitus, 2, 82-87. doi: 10.4236/jdm.2012.21013.

[1]   Parkin, C.G. and Brooks, N. (2002) Is postprandial glucose control important? Is it practical in primary care settings? Clinical Diabetes, 20, 71-76. doi:10.2337/diaclin.20.2.71

[2]   ADA (2001) Postprandial blood glucose. Diabetes Care, 24, 775-778. doi:10.2337/diacare.24.4.775

[3]   Hanefeld, M., Schmechel, H., Julius, U. and Schwanebeck, U. (1996) Determinants for coronary heart disease in non-insulin-dependent diabetes mellitus: Lessons from the diabetes intervention study. Diabetes Research and Clinical Practice, 30, 67-70. doi:10.1016/S0168-8227(96)80040-4

[4]   Ortiz-Andrade, R.R., Garcia-Jimenez, S., Castillo-Espana, P., Ramirez-Avila, G., Villalobos-Molina, R. and Estrada-Soto, S. (2007) alpha-Glucosidase inhibitory activity of the methanolic extract from Tournefortia hartwegiana: An anti-hyperglycemic agent. Journal of Ethnopharmacology, 109, 48-53.

[5]   Monnier, L. and Colette, C. (2006) Contributions of fasting and postprandial glucose to hemoglobin A1c. Endocrinology Practice, 12, 42-46.

[6]   Van de Laar, F.A. (2008) Alpha-glucosidase inhibitors in the early treatment of type 2 diabetes. Vascular Health Risk Management, 4, 1189-1195.

[7]   Van de Laar, F.A., Lucassen, P.L., Akkermans, R.P., van de Lisdonk, E.H., et al. (2005) Alpha-glucosidase inhibitors for patients with type 2 diabetes: Results from a Cochrane systematic review and meta-analysis. Diabetes Care, 28, 154-163. doi:10.2337/diacare.28.1.154

[8]   Matsuda, H., Nishida, N. and Yoshikawa, M. (2002) Antidiabetic principles of natural medicines. V. Aldose reductase inhibitors from Myrcia multiflora DC. (2): Structures of myrciacitrins III, IV, and V. Chemistry and Pharmacy Bulletin, 50, 429-431. doi:10.1248/cpb.50.429

[9]   Adewole, S.O., Caxton-Martins, E.A. and Ojewole, J.A.O. (2006) Protective effect of quercetin on the morphology of pancreatic β-cells of streptozotocin-treated diabetic rats. African Journal of Traditional Complementary and Alternative Medicine, 4, 64-74.

[10]   Shetty, A.K., Rashmi, R., Rajan, M.G.R., Sambaiah, K. and Salimath P.V. (2004) Antidiabetic influence of quercetin in streptozotocin-induced diabetic rats. Nutrition Research, 24, 373-381. doi:10.1016/j.nutres.2003.11.010

[11]   Anjaneyulu, M. and Chopra, K. (2004) Quercetin, an anti-oxidant bioflavonoid, attenuates diabetic nephropathy in rats. Clinical and Experimental Pharmacology and Physiology, 31, 244-248. doi:10.1111/j.1440-1681.2004.03982.x

[12]   Coskun, O., Kanter, M., Korkmaz, A. and Oter, S. (2005) Quercetin, a flavonoid antioxidant, prevents and protects streptozotocin-induced oxidative stress and β-cell damage in rat pancreas. Pharmacological Research, 51, 117-123. doi:10.1016/j.phrs.2004.06.002

[13]   Kobori, M., Masumoto, S., Akimoto, Y. and Takahashi, Y. (2009) Dietary quercetin alleviates diabetic symptoms and reduces streptozotocin-induced disturbance of hepatic gene expression in mice. Molecular Nutrition and Food Research, 53, 859-868. doi:10.1002/mnfr.200800310

[14]   Youl, E., Bardy, G., Magous, R., Cros, G., Sejalon, F., Virsolvy, A., et al. (2010) Quercetin potentiates insulin secretion and protects INS-1 pancreatic β-cells against oxidative damage via the ERK1/2 pathway. British Journal Pharmacology, 161, 799-814. doi:10.1111/j.1476-5381.2010.00910.x

[15]   Anjaneyulu, M. and Chopra, K. (2004) Quercetin attenuates thermal hyperalgesia and cold allodynia in STZ-induced diabetic rats. Indian Journal of Experimental Biology, 42, 766-769.

[16]   Ishikawa, A., Yamashita, H., Hiemori, M., Inagaki, E., Kimoto, M., Okamoto, M., et al. (2007) Characterization of inhibitors of postprandial hyperglycemia from the leaves of Nerium indicum. Journal of Nutrition Science and Vitaminology, 53, 166-173. doi:10.3177/jnsv.53.166

[17]   Jo, S.H., Ka, E.H., Lee, H.S., Apostolidis, E., Jang, H.D. and Kwon, Y.I. (2009) Comparison of antioxidant potential and rat intestinal α-glucosidases inihibitory activities of quercetin, rutin, and isoquercetin. International Journal of Applied Research and Natural Products, 2, 52-60.

[18]   Meng, X., Maliakal, P., Lu, H., Lee, M.J. and Yang, C.S. (2004) Urinary and plasma levels of resveratrol and quercetin in humans, mice, and rats after ingestion of pure compounds and grape juice. Journal of Agriculture and Food Chemistry, 52, 935-942. doi:10.1021/jf030582e

[19]   Sima, A.A. and Chakrabarti, S. (1992) Long-term suppression of postprandial hyperglycaemia with acarbose retards the development of neuropathies in the BB/W-rat. Diabetologia, 35, 325-330. doi:10.1007/BF00401199

[20]   Monnier, L., Mas, E., Ginet, C., Michel, F., Villon, L., Cristol, J. P. and Colette, C. (2006) Activation of oxidative stress by acute glucose fluctuations compared with sustained chronic hyperglycemia in patients with type 2 diabetes. Journal of American Medical Association, 295, 1681-1687. doi:10.1001/jama.295.14.1681

[21]   Aryangat, A.V. and Gerich, J.E. (2010) Type 2 diabetes: Postprandial hyperglycemia and increased cardiovascular risk. Vascular Health Risk Management, 6, 145-155.

[22]   Dahlqvist, A. and Borgstrom, B. (1961) Digestion and absorption of disaccharides in man. Biochemical Journal, 81, 411-418.

[23]   Hogan, S., Zhang, L., Li, J., Sun, S., Canning, C. and Zhou, K. (2010) Antioxidant rich grape pomace extract suppresses postprandial hyperglycemia in diabetic mice by specifically inhibiting alpha-glucosidase. Nutrition and Metabolism, 7, 71. doi:10.1186/1743-7075-7-71

[24]   Pospisilik, J.A., Martin, J., Doty, T., Ehses, J.A., Pamir, N., Lynn, F.C., et al. (2003) Dipeptidyl peptidase IV inhibitor treatment stimulates beta-cell survival and islet neogenesis in streptozotocin-induced diabetic rats. Diabetes, 52, 741-750. doi:10.2337/diabetes.52.3.741

[25]   Kim, J.H., Kang, M.J., Choi, H.N., Jeong, S.M., Lee, Y.M. and Kim, J.I. (2011) Quercetin attenuates fasting and postprandial hyperglycemia in animal models of diabetes mellitus. Nutrition Research Practice, 5, 107-111.

[26]   Lee, S.S., Lin, H.C. and Chen, C.K. (2007) Acylated flavonol monorhamnosides, alpha-glucosidase inhibitors, from Machilus philippinensis. Phytochemistry, 69, 2347-2353. doi:10.1016/j.phytochem.2008.06.006

[27]   Jong-Anurakkun, N., Bhandari, M.R. and Kawabata, J. (2007) alpha-glucosidase inhibitors from Devil tree (Alstonia scholaris). Food Chemistry, 103, 1319-1323. doi:10.1016/j.foodchem.2006.10.043