ABSTRACT The ability of seed extracts of Trigonella foenum-graecum (T. foenum-graecum) and Cuminum cyminum (C. cyminum) to act as natural coagulants was tested using natural turbid water. Seed extracts were prepared using distilled water and NaCl (0.5 M and 1.0 M) solution. Only 1.0 M NaCl extract of T. foenum-graecum had coagulation capability and did not depend on pH values. Further it showed that natural coagulant obtained from T. foenum-graecum is temperature (upto 100?C) and pH stable (pH 4.0 - 10.0). Extract of C. cyminum had very minimal (16 ± 2) coagulation property. The seed extract of T. foenum-graecum showed about 80% coagulation properties, where as the best known natural coagulants such as Strychnos potatorum and Moringa oleifera, and chemical coagulant such as Al2(SO4)3 showed around 90%, 65% and 95% respectively, which are used as standards for the present study. When compared with pond water, T. foenum-graecum extract treated water shows decrease in alkalinity, turbidity, KMnO4 demand and total coli-form. This study reveals that seed extract of T. foenum-graecum can be used as natural water coagulant.
Cite this paper
Ramamurthy, C. , Maheswari, M. , Selvaganabathy, N. , Kumar, M. , Sujatha, V. and Thirunavukkarasu, C. (2012) Evaluation of eco-friendly coagulant from Trigonella foenum-graecum seed. Advances in Biological Chemistry, 2, 58-63. doi: 10.4236/abc.2012.21007.
  WHO (World Health Organization) (1997) Surveillance and control of community supplies. In: Guidelines for drinking-water quality, World Health Organization, Geneva.
 Beltran-Heredia, J. and Sanchez-Martin, J. (2008) Azo dye removal by Moringa oleifera seed extract coagulation. Coloration Technology, 124, 310-317.
 Folkard, G. and Sutherland, J. (2002) Development of a naturally derived coagulant for water and wastewater treat- ment. Water Science and Technology: Water Supply, 2, 89- 94.
 Ndabigengesere, A. and Narasiah, K.S. (1998) Quality of water treated by coagulation using Moringa oleifera seeds. Water Research, 32, 781-791.
 Babu, R. and Chaudhuri, M. (2005) Home water treatment by direct filtration with natural coagulant. Journal of Water and Health, 3, 27-30.
 Tripathi, P.N., Chaudhury, M. and Bokil, S.D. (1976) Nirmali seed—A naturally occurring coagulant. Indian Journal of Environmental Health, 18, 72-81.
 Lu, F.R., Shen, L., Qin, Y., et al. (2008) Clinical observation on Trigonella foenum-graecum L. total saponins in combination with sulfonylureas in the treatment of type 2 diabetes mellitus. Chinese Journal of Integrative Medi- cine, 14, 56-60. doi:10.1007/s11655-007-9005-3
 Petit, P., Sauvaire, Y., Ponsin, G., et al. (1993) Effects of a fenugreek seed extract on feeding behaviour in the rat: Metabolic-endocrine correlates. Pharmacology Biochemis- try and Behavior, 45, 369-374.
 Ahmadiani, A., Jayan, M., Semnanjan, S., et al. (2001) Anti-inflammatory and antipyritic effects of Trigonella foenum-graecum leaves extract in the rat. Journal of Eth- nopharmarcology, 75, 283-286.
 Randhir, R., Lin, Y.T. and Shetty, K. (2004) Phenolics, their antioxidant and antimicrobial activity in dark ger- minated fenugreek sprouts in response to peptide and phytochemical elicitors. Asia Pacific Journal of Clinical Nutrition, 13, 295-307.
 Broca, C., Manteghetti, M., Gross, R., et al. (2000) 4- Hydroxyisoleucine: Effects of synthetic and natural analogues on insulin secretion. European Journal of Pharmacology, 39, 339-345.
 Raju, J., Patlolla, M.R., Swamy, M.V., et al. (2004) Diosgenin, a steroid saponin of Trigonella foenum grae- cum (Fenugreek), inhibits azoxymethane-induced aberrant crypt foci formation in F344 rats and induces apoptosis in HT-29 human colon cancer cells. Cancer Epidemiology Biomarkers and Prevention, 13, 1392-1398.
 Sowmya, P. and Rajyalakshmi, P. (1999) Hypocholesterolemic effect of germinated fenugreek seeds in human subjects. Plant Foods for Human Nutrition, 53, 359-365.
 Srinivasan, K. (2005) Plant foods in the management of diabetes mellitus: Spices as beneficial antidiabetic food adjuncts. International Journal of Food Sciences and Nutrition, 56, 399-414. doi:10.1080/09637480500512872
 Pradeep, K.U. and Geervani, P. (1994) Influence of spices on protein utilisation of winged bean (Psophocarpus tetragonolobus) and horsegram (Dolichos biflorus). Plant Foods for Hu-man Nutrition, 46, 187-193.
 Beltran-Heredia, J. and Sanchez-Martin, J. (2009) Removal of sodium lauryl sulphate by coagulation/floccu- lation with Moringa oleifera seed extract. Journal of Ha- zardous Materials, 164, 713-719.
 Beltran-Heredia, J. and Sanchez-Martin, J. (2009) Improvement of water treatment pilot plant with Moringa oleifera extract as flocculant agent. Environmental Technology, 30, 525-534. doi:10.1080/09593330902831176
 Trease, G.E. and Evans, W.C. (1978) Pharmacology. Bai- lliere Tindall Ltd., London, 60-75.
 Lowry, O.H., Rosebrough, N.J., Farr, A.L., et al. (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193, 265-275.
 Clesceri, L.S., Greenberg, A.E. and Eaton, A.D. (1998) Standard methods for the examination of water and wastewater. In: APHA, AWWA and WEF, Washington DC.
 Selbstverlag der MEBAK and Freising-Weihenstephan. (1997) Analytical methods in brewing industry-MEBAK, Germany.
 Ghebremichael, K.A., Gunaratna, K.R. and Dalhammar, G. (2006) Single-step ion exchange purification of the coagulant protein from Moringa oleifera seed. Applied Microbiology and Biotechnology, 70, 526-532.
 Ndabigengesere, A., Narasiah, K.S. and Talbot, B.G. (1995) Active agents and mechanism of coagulation of turbid waters using Moringa oleifera. Water Research, 29, 703-710. doi:10.1016/0043-1354(94)00161-Y
 Ghebremichael, K.A., Gunaratna, K.R., Henriksson, H., et al. (2005) A simple purification and activity assay of the coagulant protein from Moringa oleifera seed. Water Research, 39, 2338-2344.
 Okuda, T., Baes, A.U., Nishijima, W., et al. (2001) Coagulation mechanism of salt solution-extracted active com- ponent in Moringa oleifera seeds. Water Research, 35, 830-834. doi:10.1016/S0043-1354(00)00296-7
 Sanghi, R., Bhattacharya, B. and Singh, V. (2002) Cassia angustifolia seed gum as an effective natural coagulant for deco-lourisation of dye solutions. Green Chemistry, 4, 252-254. doi:10.1039/b200067a
 Okuda, T., Baes, A.U., Nishijima, W., et al. (2001) Isolation and characterization of coagulant extracted from Mo- ringa oleifera seed by salt solution. Water Research, 35, 405-410. doi:10.1016/S0043-1354(00)00290-6
 Diaz, A., Rincon, N., Escorihuela, A., et al. (1999) A preliminary evaluation of turbidity removal by natural coagulants indigenous to Venezuela. Process Biochemis- try, 35, 391-395. doi:10.1016/S0032-9592(99)00085-0
 ?c′iban, M., Klasnja, M.T. and Stojimirovic, J.L. (2005) Investigation of coagulation activity of natural coagulants from seeds of different leguminous species. Acta Perio- dica Technologica, 36, 81-87.
 EPA (2003) National secondary drinking water regulations, EPA/816/F-03-016, Washington DC.