ABB  Vol.3 No.1 , February 2012
A microliter incubator array for understanding culture condition selectivity
ABSTRACT
The emergence of antibiotic-resistant bacterial strains and the weaponization of rare bacterial strains pose a potential threat of pandemic disease. Those involved in the treatment and control of infectious diseases have called for the development of a device capable of rapidly, simultaneously, and safely investigate a myriad of culture conditions. In response to this need, a microliter incubator array system is described and results from a proof-of-concept study using yeast cells to determine optimal growth conditions is presented.

Cite this paper
Fulstone, R. , Coughlan, C. , Wiktorowicz, J. and Lengsfeld, C. (2012) A microliter incubator array for understanding culture condition selectivity. Advances in Bioscience and Biotechnology, 3, 87-91. doi: 10.4236/abb.2012.31013.
References
[1]   Lederberg, J. (2000) Infectious history. Science, 288, 287-293. doi:10.1126/science.288.5464.287

[2]   Hammerschmidt, S., Hacker, J. and Klenk, H.-D. (2005) Threat of infection: Microbes of high pathogenic potential—Strategies for detection, control and eradication. International Journal of Medical Microbiology, 295, 141- 151. doi:10.1016/j.ijmm.2005.03.004

[3]   Fauci, A.S. (2006) Emerging and re-emerging infectious diseases: Influenza as a prototype of the host-pathogen balancing act. Cell, 124, 665-670. doi:10.1016/j.cell.2006.02.010

[4]   Rino, R. (2001) Reverse vaccinology, a genome-based approach to vaccine development. Vaccine, 19, 2688- 2691. doi:10.1016/S0264-410X(00)00554-5

[5]   Raoult, D., et al. (2000) Cultivation of the bacillus of Whipple’s Disease. New England Journal of Medicine, 342, 620-625. doi:10.1056/NEJM200003023420903

[6]   Mora, M., et al. (2003) Reverse vaccinology. Drug Discovery Today, 8, 459-464. doi:10.1016/S1359-6446(03)02689-8

[7]   M?kel?, P.H. (2000) Vaccines, coming of age after 200 years. FEMS Microbiology Reviews, 24, 9-20.

[8]   Rappuoli, R. and Covacci, A. (2003) Reverse vaccinology and genomics. Science, 302, 602. doi:10.1126/science.1092329

[9]   Baron, M.D., et al. (2005) The Plowright vaccine strain of Rinderpest virus has attenuating mutations in most genes. Journal of General Virology, 86, 1093-1101. doi:10.1099/vir.0.80751-0

[10]   Roop, R.M. (1999) Identification of secondary mutations which enhance and stabilize the attenuation of brucella HTRA Mutants: Improving brucella HTRA-based strains as vaccine. University Medical Center, Louisiana State University, Shreveport.

[11]   Cookson, S., et al. (2005) Monitoring dynamics of single-cell gene expression over multiple cell cycles. Molecular Systems Biology, 1, 1-6. doi:10.1038/msb4100032

[12]   Groisman, A., et al. (2005) A microfluidic chemostat for experiments with bacterial and yeast cells. Nature Methods, 2, 685-689. doi:10.1038/nmeth784

[13]   Sparvero, L.J., et al. (2007) Proteomic analysis of the amyloid precursor protein fragment C99: Expression in yeast. Analytical Biochemistry, 370, 162-170. doi:10.1016/j.ab.2007.07.033

 
 
Top