ABB  Vol.3 No.1 , February 2012
Adsorption of cytochrome c by succinic anhydride-modified peanut shells
Abstract: A novel adsorbent based on peanut shells modified with glutaraldehyde and succinic anhydride was prepared. Factors affecting the adsorption capacity, such as the pH, temperature, adsorption time, initial cytochrome c (cyt c) concentration and NaCl ionic strength, were extensively investigated. The results showed that the maximum adsorption capacity of the modified peanut shells (MPSs) was 432.6 mg/g when 10 mL of cyt c solution was adsorbed by 20 mg of MPSs at pH 5.0 for 3 h. In contrast, the adsorption capacities of the unmodified peanut shells (PSs), alkaline peanut shells (APSs) and crosslinked peanut shells (CPSs) were only 100.6, 180.3, and 173.0 mg/g, respectively, 4.3-, 2.4-, and 2.5-fold lower, respectively, than that of the modified shells. The desorption rate reached 89.9% with 1.5 mol/L NaCNS as an eluent, because the electrostatic attraction between the positive charges of the protein and the negative charges of the MPSs was reduced when the ionic strength was increasing. The MPSs were used to separate and purify cytochrome c from pig myocardium. A purification of 13.5-fold in a single step with a total enzyme activity recovery of 74.0% was achieved.
Cite this paper: Li, L. , Sun, X. and Li, B. (2012) Adsorption of cytochrome c by succinic anhydride-modified peanut shells. Advances in Bioscience and Biotechnology, 3, 14-19. doi: 10.4236/abb.2012.31003.

[1]   Turkmen, D., Yavuz, H. and Denizli, A. (2006) Synthesis of tentacle type magnetic beads as immobilized metal chelate affinity support for cytochrome c adsorption. International Journal of Biological Macromolecules, 38, 126-133.

[2]   Jang, J.H. and Lim, H.B., (2010) Characterization and analytical application of surface modified magnetic nanoparticles. Microchemical Journal, 94, 148-158. doi:10.1016/j.microc.2009.10.011

[3]   Akkaya, B., Uzun, L., Candan, F. and Denizli, A. (2007) N-methacryloyl-(L)-histidine methyl ester carrying porous magnetic beads for metal chelate adsorption of cytochrome c. Materials Science and Engineering: C, 27, 180-187. doi:10.1016/j.msec.2006.04.009

[4]   Zhang, Q.F., Zhu, Y.B. and Li, J.P. (2009) Compared experiment on heavy metal removal by modified peanut shell and corncob. Journal of Peanut Science, 38, 6-10.

[5]   Sekine, H. (2000) Gas treatment medium using fiber assembled body and gas treatment apparatus using the medium, Jpn. Kokai Tokkyo Koho JP patent No. 2000- 237530.

[6]   Hacher, T., Roller, P. and Schmauder, R. (2000) Oil spill adsorbent based on straw, Ger. Offen. DE 19903862.

[7]   Gnanasambandam, R. and Proctor, A. (1997) Soy hull as adsorbent source in processing soy oil. Journal of the American Oil Chemists’ Society, 74, 685-692.

[8]   Zhang, L.X., Zhang, T.F. and Li, L.Y. (1997) Experimental methods and techniques of biochemistry. 2nd Edition, Higher Education Press, Beijing, 124-125.

[9]   Li, R.Q., Chen, Y., Jiang, F.Y., Fang, L. and Zhang, X.F. (2005) Measurement of the quality of purified cyt c products using kinetic spectrophotometry. Journal of Analytical Science, 21, 463-464.

[10]   Pandey, K.K. and Pitman, A.J. (2003) FTIR studies of the changes in wood chemistry following decay by brown-rot and white-rot fungi. International Biodeterioration and Biodegradation, 52, 151-160. doi:10.1016/S0964-8305(03)00052-0

[11]   Jeffrey, A.B. and Ronaid, J.S.(2002) Chemical composition and bioavailability of thermally altered pinus resinosa(red pine). Wood Organic Geochemistry, 33 1093- 1109. doi:10.1016/S0146-6380(02)00062-1

[12]   Gong, R.M., Sun, Y.Z. and Chen, J. (2005) Effect of chemical modification on dye adsorption capacity of peanut hull. Dyes and Pigments, 67, 175-181. doi:10.1016/j.dyepig.2004.12.003

[13]   Zheng, L., Dang, Z. and Yi, X. (2010) Equilibrium and kinetic studies of adsorption of Cd(II) from aqueous solution using modified corn stalk. Journal of Hazardous Materials, 176, 650-656. doi:10.1016/j.jhazmat.2009.11.081

[14]   Richard, S., Blackburm, H.A. and Lorna, L.K. (2006) Sorption of poly (hexamethylenebiguanide) on cellulose: Mechanism of binding and molecular recognition. Langmuir, 22, 5636-5644. doi:10.1021/la053002b

[15]   Tan, I.A.W., Hameed, B.H. and Ahmad, A.L. (2007) Equilibrium and kinetic studies on basic dye desorption by oil palm fiber activated carbon. Chemical Engineering Journal, 27, 111-119. doi:10.1016/j.cej.2006.09.010