ABSTRACT We investigated the effect of silver-loaded zeolite (Ag-Z) on antibacterial activity of some antibiotics against Es-cherichia coli against antibiotics. We treated the bacterial suspension of E. coli by Ag-Z to be the level which the decrease of viable cell count was not observed. Susceptibility test of E. coli treated with Ag-Z was carried out against four antibiotics with different mode of action. Apparent increase in susceptibility was found in the case of rifampicin, not in others. The result suggests that application of Ag-Z to ward environment might give a good outcome at the treatment with rifampicin and at the treatment for pathogenic microbe spread through the air, such as tubercle bacilli.
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Y. Inoue and H. Hamashima, "Effect of Silver-Loaded Zeolite on the Susceptibility of Escherichia Coli Against Antibiotics," Journal of Biomaterials and Nanobiotechnology, Vol. 3 No. 1, 2012, pp. 114-117. doi: 10.4236/jbnb.2012.31015.
 R. L. Davies and S. F. Etris, “The Development and Functions of Silver in Water Purification and Disease Control,” Catalysis Today, Vol. 36, No. 1, 1997, pp. 107- 114. doi:10.1016/S0920-5861(96)00203-9
 R. M. Slawson, H. Le and J. T. Trevors, “Bacterial Interactions with Silver,” Biology of Metals, Vol. 3, No. 3-4, 1990, pp. 151-154. doi:10.1007/BF01140573
 K. Hiyama, N. Moriyasu, T. Omori, O. Miyagawa, Y. Seino and Y. Goto, “Antibacterial Efficiency of Ag- and Zn-Zeolites and the Bactericidal Action of Ag- and Zn-Zeolite-Kneaded Polyethylene Films,” Journal of Antibacterial and Antifungal Agents, Vol. 23, No. 4, 1995, pp. 197-203.
 K. C. Im, Y. Takasaki, A. Endo and M. Kuriyama, “Antibacterial Activity of A-Type Zeolite Supporting Silver Ions in Deionized Distilled Water,” Journal of Antibacterial and Antifungal Agents, Vol. 24, No. 5, 1996, pp. 269-274.
 H. Kourai, Y. Manabe and Y. Yamada, “Mode of Bactericidal Action of Zirconium Phosphate Ceramics Containing Silver Ions in the Crystal Structure,” Journal of Antibacterial and Antifungal Agents, Vol. 22, No. 10, 1994, pp. 595-601.
 Y. Inoue, M. Hoshino, H. Takahashi, T. Noguchi, T. Murata, Y. Kanzaki, H. Hamashima and M. Sasatsu, “Bactericidal Activity of Ag-Zeolite Mediated by Reactive Oxygen Species under Aerated Conditions,” Journal of Inorganic Biochemistry, Vol. 92, No. 1, 2002, pp. 37-42.
 H. He, X. Dong, M. Yang, Q. Yang, S. Duan, Y. Yu, J. Han, C. Zhang, L. Chen and X. Yang, “Catalytic Inactivation of SARS Coronavirus, Escherichia coli and Yeast on Solid Surface,” Catalysis Communication, Vol. 5, No. 3, 2004, pp. 170-172. doi:10.1016/j.catcom.2003.12.009
 H. L. Pape, F. Solano-Serena, P. Contini, C. Devillers, A. Maftah and P. Leprat, “Involvement of Reactive Oxygen Species in the Bactericidal Activity of Activated Carbon Fiber Supporting Silver: Bacterial Activity of ACF (Ag) Mediated by ROS,” Journal of Inorganic Biochemistry, Vol. 98, No. 6, 2004, pp. 1054-1060.
 I. Sondi and B. Salopek-Sondi, “Silver Nanoparticles as Antimicrobial Agent: A Case Study on E. coli as a Model for Gram-Negative Bacteria,” Journal of Colloid and Interface Sciences, Vol. 275, No. 1, 2004, pp. 177-182.
 E. Katori, T. Nagano, T. Kunieda and M. Hirobe, “Facile Desulfurization of Thiocarbonyl Groups to Carbonyls by Superoxide. A Model of Metabolic Reactions,” Chemical & Pharmaceutical Bulletin, Vol. 29, No. 10, 1981, pp. 3057-3077. doi:10.1248/cpb.29.3075
 M. Yamanaka, K. Hara and J. Kudo, “Bactericidal Actions of a Silver Ion Solution on Escherichia coli, Studied by Energy-Filtering Transmission Electron Microscopy and Proteomic Analysis,” Applied Environmental Micro biology, Vol. 71, No. 11, 2005, pp. 7589-7593.
 M. Sokmen, F. Candan and Z. Sumer, “Disinfection of E. coli by the Ag-TiO2/UV System: Lipidperoxdation,” Journal of Photo-chemistry and Photobiology A, Vol. 143, No. 2-3, 2001, pp. 241-244.
 Y. Matsumura, K. Yoshikata, S. Kunisaki and T. Tsuchido, “Mode of Bactericidal Action of Silver Zeolite and Its Comparison with That of Silver Nitrate,” Applied Environmental Microbiology, Vol. 69, No. 7, 2003, pp. 4278-4281. doi:10.1128/AEM.69.7.4278-4281.2003
 Q. L. Feng, J. Wu, G. Q. Chen, F. Z. Cui and J. O. Kim, “A mechanistic Study of the Antibacterial Effect of Silver Ion on Escherichia coli and Staphylococcus aureus,” Journal of Biomedical Materials Research, Vol. 52, No. 4, 2000, pp. 662-668.