AJAC  Vol.3 No.1 , January 2012
Fast Determination of 22 Pesticides in Rice Wine by Dispersive Solid-Phase Extraction in Combination with GC-MS
Abstract: A rapid method was developed for the determination of 22 pesticides in rice wine. The procedure involved an extraction with acetonitrile and a cleanup step using dispersive solid-phase extraction (d-SPE), and primary-secondary amine (PSA) and octadecylsilane (ODS) were used as sorbents. D-SPE had some advantages over some traditional prepara- tions, especially in time and cost. Both the extraction and cleanup only cost about 15 min per a sample. Then the GC-MS was used for quantitative and qualitative analysis. Matrix-matched standards solution and analyte protectant were compared to decrease the matrix effect, and the former showed a better efficacy. Acceptable linearity was achieved in the range of 0.05 - 0.30 mg/L. After matrix-matched standards calibration, recoveries were between 60 and 140%. For the most of pesticides, precision and repeatability were less than 10% and 16%, respectively. The result indicated that the developed method was suitable for the determination of the multi-pesticides in rice wine.
Cite this paper: N. Chen, H. Gao, N. Ye, Q. Zhong, Z. Xiong and X. Gu, "Fast Determination of 22 Pesticides in Rice Wine by Dispersive Solid-Phase Extraction in Combination with GC-MS," American Journal of Analytical Chemistry, Vol. 3 No. 1, 2012, pp. 33-39. doi: 10.4236/ajac.2012.31006.

[1]   H. Yu, Y. Ying, X. Fu and H. Lu, “Quality Determination of Chinese Rice Wine Based on Fourier Transform Near Infrared Spectroscopy,” Journal of Near Infrared Spec- troscopy, Vol. 14, No. 1, 200, pp. 37-44. doi:10.1255/jnirs.584

[2]   D. T. Likas, N. G. Tsiropoulos and G. E. Miliadis, “Rapid Gas Chromatographic Method for the Determination of Famoxadone, Trifloxystrobin and Fenhexamid Residues in Tomato, Grape and Wine Samples,” Journal of Chro- matography A, Vol. 1150, No. 1-2, 2007, pp. 208-214. doi:10.1016/j.chroma.2006.08.041

[3]   A. Economou, H. Bo-titsi, S. Antoniou and D. Tsipi, “De- termination of Multi-class Pesticides in Wines by Solid- phase Extraction and Liquid Chromatography-Tandem Mass Spectrometry,” Journal of Chromatography A, Vol. 1216, No. 31, 2009, pp. 5856-5867. doi:10.1016/j.chroma.2009.06.031

[4]   J. J. Jimenez, J. L. Bernal, M. J. Del Nozal, L. Toribio and J. Bernal, “Use of SPE—GC/EIMS for Residue Ana- lysis in Wine Elaborated from Musts Spiked with Formu- lations of Chlorpyri-phos—Methyl, Methiocarb, Dicofol, and Cyproconazol,” Journal of Separation Science, Vol. 30, No. 4, 2007, pp. 547-556. doi: 10.1002/jssc.200600345

[5]   I. Carpinteiro, M. Ramil, I. Rodríguez and R. Cela, “Determination of Fungicides in Wine by Mixed-mode Solid Phase Extraction and Liquid Chromatography Coupled to Tandem Mass Spectrometry,” Journal of Chromatography A, Vol. 1217, No. 48, 2010, pp. 7484-7492. doi:10.1016/j.chroma.2010.09.080

[6]   G. F. Pang, C. L. Fan, Y. M. Liu, Y. Z. Cao, J. J. Zhang, B. L. Fu, X. M. Li, Z. Y. Li and Y. P. Wu, “Multi-Resi- due Method for the Determination of 450 Pesticide Resi- dues in Honey, Fruit Juice and Wine by Double-Cartridge Solid-Phase Extraction/Gas Chromatogra-phy-Mass Spectrometry and Liquid Chromatography-Tandem Mass Spec- trometry,” Food Additives & Contaminants: Part A, Vol. 23, No. 8, 2006, pp. 777-810. doi:10.1080/02652030600657997

[7]   D. Zhan, R. E. Anli, N. Vural and M. Bayram, “Determination of Chloroanisoles and Chlorophenols in Cork and Wine by Using HS-SPME and GC-ECD Detection,” Jour- nal of the Institute of Brewing, Vol. 115, No. 1, 2009, pp. 71-77.

[8]   L. M. Ravelo-Pérez, J. Hernández-Borges, T. M. Borges- Miquel and Má Rodríguez-Delgado, “Solid-Phase Mi- croextraction and Sample Stacking Micellar Electroki- netic Chromatography for the Analysis of Pesticide Re- sidues in Red Wines,” Food Chemistry, Vol. 111, No. 3, 2008, pp. 764-770. doi:10.1016/j.foodchem.2008.04.020

[9]   M. Anastassiades, S. J. Lehotay, D. Tajnbaher and F. J. Schenck, “Fast and Easy Multiresidue Method Employing Acetonitrile Extrac-tion/Partitioning and ‘Dispersive Solid- Phase Extraction’ for the Determination of Pesticide Re- sidues in Produce,” Journal of AOAC International, Vol. 86, No. 2, 2003, pp. 412-431.

[10]   J. Dong, X. M. Gong, L. Zhang and H. T. Wang, “Determination of Imidacloprid, Tebufenozide, Avennectins and Hexythiazox in Vegetables by QuEChERS-HPLC,” Chi- nese Journal of Analysis Laboratory, Vol. 3, 2008, pp. 91-94.

[11]   S. H. Patil, K. Banerjee, S. Dasgupta, D. P. Oulkar, S. B. Patil, M. R. Jadhav, R. H. Savant, P. G. Adsule and M. B. Deshmukh, “Multiresidue Analysis of 83 Pesticides and 12 Dioxin-like Polychlorinated Biphenyls in Wine by Gas Chro-matography-Time-of-Flight Mass Spectrometry,” Jour- nal of Chromatography A, Vol. 1216, No. 12, 2009, pp. 2307-2319. doi:10.1016/j.chroma.2009.01.091

[12]   K. Zhang, J. W. Wong, D. G. Hayward, P. Sheladia, A. J. Krynitsky, F. J. Schenck, M. G. Webster, J. A. Ammann and S. E. Ebeler, “Multiresidue Pesticide Analysis of Wines by Dispersive Solid-Phase Extrac-tion and Ultra- high-Performance Liquid Chromatogra-phy-Tandem Mass Spectrometry,” Journal of Agricultural and Food Chem- istry, Vol. 57, No. 10, 2009, pp. 4019-4029. doi:10.1021/jf9000023

[13]   S. C. Cunha, J. O. Fernandes, A. Alves and M. Oliveira, “Fast Low-pressure Gas Chromatogra-phy-Mass Spectro- metry Method for the Determination of Multiple Pesti- cides in Grapes, Musts and Wines,” Journal of Chroma- tography A, Vol. 1216, No. 1, 2009, pp. 119-126. doi:10.1016/j.chroma.2008.11.015

[14]   X. Xu, L. Li, W. Zhong and Y. He, “Rapid GC-MS Ana- lysis of Pesticide Residues Using Analyte Protectants,” Analytical Letters, Vol. 42, No. 16, 2009, pp. 2578-2591. doi:10.1080/00032710903243646

[15]   J. M. Lee, J. W. Park, G. C. Jang and K. J. Hwang, “Com- parative Study of Pesticide Multi-Residue Extraction in Tobacco for Gas Chromatogra-phy—Triple Quadrupole Mass Spectrometry,” Journal of Chromatography A, Vol. 1187, No. 1, 2008, pp. 25-33. doi:10.1016/j.chroma.2008.02.035

[16]   Y. Jiang, X. Li, J. Xu, C. Pan, J. Zhang and W. Niu, “Multiresidue Method for the Determination of 77 Pes- ticides in Wine Using QuEChERS Sample Preparation and Gas Chromatography with Mass Spec-trometry,” Food Additives & Contaminants: Part A, Vol. 26, No. 6, 2009, pp. 859-866. doi:10.1080/02652030902822794

[17]   W. Shen, K. Yu, Q. Gui, Y. Jiang, Z. Zhao, C. Shen, B. Wu and X. Chu, “Determination of 107 Pesticide Resi- dues in Vegetables Using Off-Line Dispersive Solid- phase Extraction and Gas Chroma-tography-tandem Mass Spectrometry,” Chinese Journal of Chromatography, Vol. 4, 2009, pp. 391-400.

[18]   P. Payá, M. Anastassiades, D. Mack, I. Sigalova, B. Tas- delen, J. Oliva and A. Barba, “Analysis of Pesticide Re- sidues Using the Quick Easy Cheap Effective Rugged and Safe (QuEChERS) Pesticide Multiresidue Method in Com- bination with Gas and Liquid Chromatography and Tan- dem Mass Spectrometric Detection,” Analytical and Bioanalytical Chemistry, Vol. 389, No. 6, 2007, pp. 1697- 1714. doi:10.1007/s00216-007-1610-7

[19]   C. Le-sueur, P. Knittl, M. Gartner, A. Mentler and M. Fuerhacker, “Analysis of 140 Pesticides from Conven- tional Farming Foodstuff Samples after Extraction with the Modified QuECh-eRS Method,” Food Control, Vol. 19, No. 9, 2008, pp. 906-914. doi:10.1016/j.foodcont.2007.09.002

[20]   T. D. Nguyen, J. E. Yu, D. M. Lee and G. H. Lee, “A Multiresidue Method for the Determination of 107 Pe- sticides in Cabbage and Radish Using QuEChERS Sam- ple Preparation Method and Gas Chromato-graphy Mass Spectrometry,” Food Chemistry, Vol. 110, No. 1, 2008, pp. 207-213. doi:10.1016/j.foodchem.2008.01.036

[21]   A. Garrido Frenich, J. L. Martínez Vidal, E. Pastor- Montoro and R. Romero-González, “High-Throughput Determination of Pesticide Residues in Food Commodi- ties by Use of Ultra-performance Liquid Chromatography —Tandem Mass Spectrometry,” Analytical and Bioanalytical Chemistry, Vol. 390, No. 3, 2008, pp. 947-959. doi:10.1007/s00216-007-1746-5

[22]   K. Ma?tovská, S. J. Le-hotay and M. Anastassiades, “Com- bination of Analyte Protec-tants to Overcome Matrix Ef- fects in Routine GC Analysis of Pesticide Residues in Food Matrixes,” Analytical Chemistry, Vol. 77, No. 24, 2005, pp. 8129-8137. doi:10.1021/ac0515576

[23]   A. Beyer and M. Biziuk, “Ap-plications of Sample Prepa- ration Techniques in the Analysis of Pesticides and PCBs in Food,” Food Chemistry, Vol. 108, 2008, pp. 669-680. doi:10.1016/j.foodchem.2007.11.024