Comparative gene expression analysis in stems of Dolichos purpureus and Arabidopsis thaliana
Affiliation(s)
Life Sciences College, South-Central University for Nationalities, Wuhan, China. CAS Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Science, Beijing, China.
Life Sciences College, South-Central University for Nationalities, Wuhan, China. CAS Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Science, Beijing, China.
ABSTRACT
The outermost layer epidermis of a plant stem plays an important role in protection and environment-sensing. The mechanisms of sensing and response to the environment through the stem epidermis remain unclear. Here we report enriched expression of genes involved in stress resistance and signal transduction functions in the stem epidermis of both D. purpureus and A. thaliana by cDNA cloning and QPCR in D. purpureus and by analysis using dataset from a genome-wide comparison with cDNAs differentially expressed between the epidermis and inner parts of top and base stem in A. thaliana. Among 188 cDNAs from the stem epidermis of D. purpureus, 13% and 17% were related to signal transduction and defense respectively. Most of them were up-regulated more in the stem epidermis than the inner stem, as well as in A. thaliana. Also, the distribution of the numbers and specificities of up-regulated genes related to signal transduction and regulatory networks in the epidermis and inner stem revealed the possibility of positional differences in regulation. The results revealed the importance of the epidermis in signal transduction and plant defence.
The outermost layer epidermis of a plant stem plays an important role in protection and environment-sensing. The mechanisms of sensing and response to the environment through the stem epidermis remain unclear. Here we report enriched expression of genes involved in stress resistance and signal transduction functions in the stem epidermis of both D. purpureus and A. thaliana by cDNA cloning and QPCR in D. purpureus and by analysis using dataset from a genome-wide comparison with cDNAs differentially expressed between the epidermis and inner parts of top and base stem in A. thaliana. Among 188 cDNAs from the stem epidermis of D. purpureus, 13% and 17% were related to signal transduction and defense respectively. Most of them were up-regulated more in the stem epidermis than the inner stem, as well as in A. thaliana. Also, the distribution of the numbers and specificities of up-regulated genes related to signal transduction and regulatory networks in the epidermis and inner stem revealed the possibility of positional differences in regulation. The results revealed the importance of the epidermis in signal transduction and plant defence.
Cite this paper
Yan, H. , Wang, X. , Chu, Y. and Dai, K. (2012) Comparative gene expression analysis in stems of Dolichos purpureus and Arabidopsis thaliana. American Journal of Molecular Biology, 2, 72-84. doi: 10.4236/ajmb.2012.21008.
Yan, H. , Wang, X. , Chu, Y. and Dai, K. (2012) Comparative gene expression analysis in stems of Dolichos purpureus and Arabidopsis thaliana. American Journal of Molecular Biology, 2, 72-84. doi: 10.4236/ajmb.2012.21008.
References
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[1] Costa, S. and Dolan, L. (2003) Epidermal patterning genes are active during embryogenesis in Arabidopsis. Development, 130, 2893-2901. doi:10.1242/dev.00493 [2] Tominaga, R., Iwata, M., Okada, K. and Wada, T. (2007) Functional analysis of the epidermal-specific MYB genes CAPRICE and WEREWOLF in Arabidopsis. Plant Cell, 19, 2264-2277. doi:10.1105/tpc.106.045732 [3] Wang, S.C., Hubbard, L., Chang, Y., Guo, J.J., Schiefelbein, J. and Chen, J.G. (2008) Comprehensive analysis of single-repeat R3 MYB proteins in epidermal cell patterning and their transcriptional regulation in Arabidopsis. BMC Plant Biology, 8, 81. [4] Chlyah, H. and Van, M.T. (1975) Distribution pattern of cell division centers on the epidermis of stem segments of torenia fournieri during de novo bud formation. Plant Physiology, 56, 28-33. doi:10.1104/pp.56.1.28 [5] Kunst, L. and Samuels, A.L. (2003) Biosynthesis and secretion of plant cuticular wax. 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Plant Physiology, 119, 1115-1124. doi:10.1104/pp.119.3.1115 [10] Hardham, A.R., Takemoto, D. and White, R.G. (2008) Rapid and dynamic subcellular reorganization following mechanical stimulation of Arabidopsis epidermal cells mimics responses to fungal and oomycete attack. Bmc Plant Biology, 8, 63. [11] Clark, A.M., Verbeke, J.A. and Bohnert, H.J. (1992) Epidermis-specific gene expression in Pachyphytum. Plant Cell, 4, 1189-1198. [12] Kryvych, S., Nikiforova, V., Herzog, M., Perazza, D. and Fisahn, J. (2008) Gene expression profiling of the different stages of Arabidopsis thaliana trichome development on the single cell level. Plant Physiology and Biochemistry, 46, 160-173. doi:10.1016/j.plaphy.2007.11.001 [13] Negri, A.S., Prinsi, B., Rossoni, M., Failla, O., Scienza, A., Cocucci, M. and Espen, L. (2008) Proteome changes in the skin of the grape cultivar Barbera among different stages of ripening. BMC Genomics, 9, 378. 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