ABSTRACT In this paper, clear liquid fermentation condition for ethanol production from Canna edulis Kerl with orthogonal design was studied. We have studied five factors that influenced clear liquid fermentation condition for ethanol production from Canna edulis Kerl by the single-factor test. The five factors were α-amylase amount, glu-coamylase amount, pH, nitrogen and auxiliary materials amount. Selected the best amounts of α-amylase, glucoamylase, pH, nitrogen source and auxiliary materials by means of the singe- factors test. And then, we used the Orthogonal test to optimize the fermentation process. The study showed that the best fermentation condi-tions were α-amylase amount 7 u/g C.eduli, glu-coamylase amount 145u/g C. edulis, pH 4.4 and the amount of urea 0.08%, 1% of rice bran as auxiliary materials. The rate of alcohol which was produced by the best fermentation conditions is 21.5% that increased by 1.3% than the rate of alcohol by the single-factor test.
Cite this paper
Shen, Y. , Tang, Q. and Wu, T. (2010) Optimization of clear liquid fermentation condition for ethanol production from Canna edulis Kerl. Natural Science, 2, 115-119. doi: 10.4236/ns.2010.22019.
 Zhu, Z.H. and Wu, T.X. (2005) Studies on characters and industral applications of Canna edulis Kerl starchy mate-ria1 [J]. Chinese Journal of Bioprocess Engineering, 3(4), 66-70.
Fang, S.Q., Zhao, Y.f., Niu, Q.C. et a1. (2005) Study on the technology of alcohol production by wheat flour clear liquid [J]. Liquor-Making Science & Technology, 5, 64-65.
Liu, L.F. and Ma. X.J. (2004) Study on alcoholic fer-mentation from wheat hourin clarifying solution [J]. Chinese Journal of henan institute of education (Natural Science Edition), 13(4), 57-58.
Wang, F.R. (1980) Analysis of industrial fermentation [M]. Beijing;China Light Industryb Press, 25-27.
Wang, F.R. (2006) Bio-engineering analysis and detec-tion [M]. Beijing: China Light Industryb Press, 140-163.
Zhang, K.C. (2004) Alcohol and tequila-technology [M]. Beijing; China Light Industryb Press.
Wu, T.X., Ding, C.Y., Yang, H.l. et a1. (2003) Study on teclmology of the semi-solid state pulp fermentation of Canna edulis Eerl for ethanol [J]. Liquor Making, 30(2), 71-73.
Fang, Y., Wang, P.H., Xie, S.Q. et a1. (2004) Technique of scarifying and fermenting process of canna starch [J]. Southwest China Journal of Agricultural Sciences, 17(2), 231-234.
Wu, T.X., Zhang, K.C., Liu, C.Z. et a1. (2005) Study on the property of canna edulis ker & its application in etha-nol fermentation [J]. Liquor-Making Science & Tech-nology, 10, 40-42.
Wu, T.X., Ding, C.Y., Yang, H.L. et a1. (2002) The fea-sibility study on the solid state fermentation and the de-velopment of alcoholic industry [J]. Food and Fermenta-tion Industries, 28(12), 67-70.
Chen, Y.F., Fang, H.Y., Wang, Z.G. et a1. (2002) Study on flask ethanol fermentation conditions of Canna edulis Kerl [J]. Liquor Making, 29(6), 63-65.
Ying, B., Qu, J.Q. and Lin, X.M. (2005) Principles of microbial technology development [M]. Chemical Indus-try Press, (3), 99-109.
Thitipraphunkul, K., Uttapap, D., Piyachomkwan, K. and Takeda, Y.A. (2003) Comparative study of edible canna (Canna edulis) starch from diferent cultivars Part I, Chemical composition and physicochemical properties [J]. Carbohydrate Polymers, 53, 3l7-324.
Shigechi, H., Fujita, Y., Koh, J., Ueda, M., Fukuda, H. and Kondo, A. (2004) Energy-saving direct ethanol pro-duction from low-temperature-cooked corn starch using a cell-surface engineered yeast strain co-displaying glu-coamylase α-amylase [J]. Biochem EngJ, 18, 149-153.
Bandaru, V.V.R., Somalanka, S.R., Menduc, D.R., Madicherla, N.R. and Chityala, A. (2006) Optimization of fermentation conditions for the production of ethanol from sago starch by co-immobilized amyloglucosidase and cells of Zymomonas mobilis using response surface methodology [J]. Enzyme Microb Technol, 38, 209-14.
Yan, L, and Shuzo, T. (2006) Ethanol fermentation from biomass resources: current state and prospects [J]. Appl Microbiol Biotechnol, 69, 627-642.
Zhu, Z.H., Zhang, H.Y. and Wu, T.X. (2007) Response surface methodology to optimize ethanol fermentation from Canna edulis Ker [J]. Liquor-Making Science &Technology, 8, 29-32.