ABSTRACT The genetic diversity of the exon2 of BoLA-DRB3 (BoLA-DRB3.2) in Chinese Holstein cattle of the south China was investigated by hemi-nested polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Six, four and eleven RFLP patterns were found after digestion with the restriction enzymes Hae III, Bst YI and Rsa I, respectively. The DNA sequence showed and twenty- five DRB3.2 alleles. GLM model analysis indicated that lactation and calving season have positive correlation with SCC (Somatic Cell Count) (p<0.01), BoLA-DRB3.2*3, *8, *18 and *26 were associated with lower SCC (p<0.01). The present findings concluded that mastitis is a severe hinder of milk production and technology. Therefore, future re-searches should focus on associations of mastitis with BoLA haplotypes rather than single BoLA genes
Cite this paper
nullWu, X. , Yang, Z. , Wang, X. , Mao, Y. , Li, S. , Shi, X. and Chen, Y. (2010) Restriction fragment length polymorphism in the exon 2 of the BoLA-DRB3 gene in Chinese Holstein of the south China. Journal of Biomedical Science and Engineering, 3, 221-225. doi: 10.4236/jbise.2010.32030.
 Andersson, L. and Davies, C.J. (1994) The major histo- compatibility complex. Cell-Mediated Immunity in Ruminants, CRC, 37-57.
Davies, C.J., Joosten, I., Andersson, L., Arriens, M.A., Bernoco, D., Bissumbhar, B., Byrns, G., van Eijk, M.J., Kristensen, B. and Lewin, H.A. (1994) Polymorphism of Bovine MHC Class II genes. Joint Report of the Fifth International Bovine Lymphocyte Antigen (BoLA) Workshop. European Journal of Immunogenetics, 21(4), 259-289.
Van Eijk, M.J., Stewart-Haynes, J.A. and Lewin, H.A. (1992) Extensive polymorphism of the BoLA-DRB3 gene distinguished by PCR-RFLP. Animal Genetics, 23, 483-496.
Do Nascimento, C.S., Machado, M.A., Martinez, M.L., da Silva, M.G.B., Martins Guimares, M.F., Campos, A.L., Sousa Azevedo, A.L., Teodoro, R.L., Silva erneque, R. and Facioni Guimare, S.E. (2006) Association of the Bovine major histocompatibility complex (BoLA) BoLA-DRB3 gene with fat and protein production and somatic cell score in Brazilian gyr dairy cattle (Bos indicus). Genetics & Molecular Biology, 29(4), 641-647.
Nassiry, M.R., Shahroodi, F.E., Mosafer, J.A., Mohammadi, A., Manshad, E., Ghazanfari, S., Mohammad Abadi, M.R. and Sulimova, G.E. (2005) Analysis and frequency of Bovine lymphocyte antigen (BoLA-DRB3) alleles in Iranian Holstein cattle. Russian Journal of Genetics, 41(6), 664-668.
Rupp, R., Hernandez, A., Mallard, B.A. (2007) Associ- ation of Bovine leukocyte antigen (BoLA) DRB3.2 with immune response, mastitis, and production and type traitsin Canadian Holsteins. Journal of Dairy Science, 90, 1029-1038.
Duangjinda, M., Buayai, D., Pattarajinda, V., Phasuk, Y., Katawatin, S., Vongpralub T. and Chaiyotvittayakul, A. (2009) Detection of Bovine leukocyte antigen DRB3 alleles as Candidate markers for clinical mastitis re- sistance in Holstein x zebu. Journal of Animal Science, 87, 469-476.
Jeseph, S. and Rusell, D.W. (2001) Molecular cloning: A laboratory manual. Cold Spring Habor Laboratory Press.
Miretti, M.M., Ferro, J.A., Lara, M.A. and Conte, E.P.B. (2001) Restriction fragment length polymorphism (RFLP) in Exon 2 of the BoLA-DRB3 gene in South American cattle. Bio Gene, 39(9-10), 311-324.
Maillard, J., Renard, C., Chardon, P., Chantal, I. and Bensaid, A. (1999) Characterization of 18 new BoLA-DRB3 alleles. Animal Genetics, 30, 200-203.
Sharif, S., Mallard, B.A., Wilkie, B.N., Sargeant, J.M., Scott, H.M., Dekkers, J.C.M. and Leslie, K.E. (1998) Association of the Bovine major histocompatibility complex DRB3 (BoLA-DRB3) alleles with occurrence of disease and milk somatic cell score in Canadian dairy cattle. Animal Genetics, 29, 185-193.