Jia Yang¹, Yueyang Yu², Bo Su³, Yun Zheng⁴, Yang Liu^2*

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¹ The Second Clinical Medical College of Yangtze University/The Radiology Department of Jingzhou Central Hospital, Jingzhou, China.
² Department of Pathogenic Biology, School of Medicine, Yangtze University, Jingzhou, China.
³ Department of Medical Morphology, School of Medicine, Yangtze University, Jingzhou, China.
⁴ Department of Medical Function, School of Medicine, Yangtze University, Jingzhou, China.
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Abstract: MicroRNAs are involved in regulation of the central nervous system (CNS) development, and miR-181d highly expressed in mature neurons. CREB is many signal pathways converged point in hippocampal neurons, and it plays a crucial role in learning and memory. In this study, we detected the negative relationship between CREB1 protein and miR-181d expression in lewis rat hippocampus development. And the bioinformatic analysis showed that, CREB1 mRNA contains complementary sequence to the miR-181d seed region. Then we further demonstrated that miR-181d controls the expression level of CREB1 gene in PC12 cells by luciferase assay and western blot. Taken together, our data demonstrated that CREB1 mRNA is the target gene of miR-181d, and conformed CREB1 protein expression was regulated by miR-181d in PC12 cells.

Keywords: miR-181d, CREB1, PC12 Cells

Cite this paper: Yang, J. , Yu, Y. , Su, B. , Zheng, Y. and Liu, Y. (2022) MiRNA-181d Is Involved in CREB1 Expression in PC12 Cells. Yangtze Medicine, 6, 13-23. doi: 10.4236/ym.2022.62002.

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