Health  Vol.2 No.1 , January 2010
The effect of high fat food on erythrocyte phospholipids, fatty acids composition and glutathione redox-system of rats with alimentary dyslipidemia
ABSTRACT
To evaluate the effects of high fat food consisted of tallow (19% of total diets) and cholesterol (2%) on modification of erythrocyte phospholipids, fatty acids composition and glutathione redox- system of male Wistar rats with alimentary dysli- pidemia. The results demonstrated that after 30 and 180 days of high-fat feed erythrocyte phos- phatidylinositol and phosphatidylcholine levels were reduced, phosphatidylserine were in-creased. Only on the 90 days of the experiment phosphatidylinositol level increased. In all grow- ups the erythrocyte 18:0 saturated fatty acids and 20:4n6, 22:4n6 polyunsaturated fatty acids (PUFA) were increased. Deficit of n3 PUFA- 20:5n3 and 22:6n3 after 90 and 180 days high fat feed promoted compensatory synthesis from 18:1n9 on 20:3n9. Erythrocyte maleic dialde-hyde increased, glutathione level decreased in all groups of rats after fed with high-fat feed. Glutathione reductase and glutathione peroxi-dase activity decreased in erythrocytes after 30 and 180 days of high-fat feed. In conclusion: high-fat diet during 30-90 days started adaptive answer in lipids of membrane and glutathione redox-system. Important mechanism of adapta-tion of a cellular membrane to high-fat diet is increase major, structuring a membrane phos-phatidylethanolamine and minor, most meta-bolic significant fractions phospholipids (phos- phatidylinositol), keeps homeostasis of 18:2n6 and 22:6n3, 20:3n9 compensatory synthesis, decrease in activity of processes lipid peroxi-dation, activation of enzymes of redox-system glutathione. But prolonging the high-fat feeding (180 days and more) formed failure compensa-tory processes (dysadaptation). It is a risk factor of developmening atherosclerosis, diabetes, steatogepatitis and other diseases.

Cite this paper
nullV. Zhukova, N. , K. Karaman, Y. and V. Zhukova, N. (2010) The effect of high fat food on erythrocyte phospholipids, fatty acids composition and glutathione redox-system of rats with alimentary dyslipidemia. Health, 2, 45-50. doi: 10.4236/health.2010.21008.
References
[1]   Jacobsen, M.U., Overvard, K., Dyerberg, J. et al. (2004) Dietary Fat and Risk of Coronary Heart Disease: Possible Effect Modification by Gender and Age. Am. J. Epidemiol, 160(2), 141-149.

[2]   Susan, M., Castracane, V., Mantzoros, S. (2004) Energy homeostasis, obesity and eating disorders: Recent Ad-vances in Endocrinology. J. Nutr, 134, 295-298.

[3]   Wymann, M.P., Schneiter, R. (2008) Lipid signaling in disease. Nature, 9, 162-176.

[4]   Denke, M.A. (2005) Diet, lifestyle and nonstatin trials: review of time to benefit. Am J Cardiol, 96, 3-10.

[5]   Hulbert, A.J., Turner, N., Storlien, L.H. et al. (2005) Dietary fats and membrane function: implications for metabolism and disease. Biol. Rev. Camb. Philos. Soc, 80(1), 155-169.

[6]   Beth, L., Marlies, K.O., Carlson, S.E. (2007) Diet (n-3) Polyunsaturated Fatty Acid Content and Parity Affect Liver and Erythrocyte Phospholipid Fatty Acid Composi-tion in Female Rats. J. Nutr, 137, 2425-2430.

[7]   Beth, L., Marlies, K.O., Susan, E. Carlson. (2007) Diet (n-3) Polyunsaturated Fatty Acid Content and Parity Af-fect Liver and Erythrocyte Phospholipid Fatty Acid Composition in Female Rats. J. Nutr, 137, 2425-2430.

[8]   Berra, B., Mortontano, G., Adouni, L. et al. (1998) Serum lipids and lipid composition of red blood cell membranes after diet with sunflower oil normal or high content of oleic acid. Riv. Ital. Sostanze Grasse, 75, 127-132.

[9]   Jula, A., Marniemi, J., Ronnemaa, T. et al. (2005) Effects of diet and simvastatin on fatty acid composition in hypercholesterolemiс men. Arterioscler Thromb Vasc Biol, 25, 1952-1959.

[10]   Poppitt, S.D., Kilmartin, P., Butler, P., Keogh, G.F. (2005) Assessment of erythrocyte phospholipid fatty acid com-position as a biomarker for dietary MUFA, PUFA or saturated fatty acid intake in a controlled cross-over in-tervention trial. Lipids Health Dis, 4, 30.

[11]   Poole, L.B., Karplus, P.A., Claiborn, A. (2004) Protein sulfenic asids in redox signaling. Annu. Rev. Pharmacol. Toxicol, 44, 325-347.

[12]   Iton, K., Ishii, T., Wakabayashi, N., Yamomoto, M. (1999) Regulatory mechanisms of cellular response to oxidative stress. Free radic. Res, 31, 319-324.

[13]   Bea, F., Hudson, F.N., Chait, A. et al. (2003) Induction of glutathione synthesis in macrophages by oxidized low- density lipoproteins is mediated by consensus antioxidant response elements. Circ. Res, 92, 386-393.

[14]   Format, H.J., Fukuto, J.M., Torres, M. (2004) Redox signaling: thiol chemistry defines which reactive oxygen and nitrogen species can act second messengers, Am. J. Physiol. Cell Physiol, 287, 246-256.

[15]   Fan, J.G., Zhong, L., Xu, Z.J. (2003) Effect of low-cal orie diet on steatohepatitis in rats with obesity and hyperlipi-demia. World J. Of Gastroenterology, 9(9), 2045-2049.

[16]   [16] European Convention for the Protection of Vertebrate Animals used for exsperimental and other scientific pur-poses. Strasburg: Council of Europe, (1986), 51.

[17]   Bligh, E.G., Dyer, W.J. (1959) A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol, 37(8), 911-917.

[18]   Svetashev, V.I., Vaskovsky, V.E. (1972) A simplified technique for thinlayer chromatography of lipids. J. Chromatogr.; 67:376-378.

[19]   Vaskovsky, V.E., Kostetsky, E.X., Vasendin, J.M. (1975) A universal reagent for phospholipids analysis. J. Chro-matogr, 111, 129-141.

[20]   Carreau, J.P., Duback, J. P. (1978) Adaptation of a mac-roscale method to the microscale for fatty acid methyl transesterification of biological lipid extract. J. Chroma-togr, 151(3), 84-390.

[21]   Stransky, K., Jursik, T., Vitek, A., Skorepa, J. (1992) An improved method of characterizing fatty acids by equivalent chain length values, J. High. Res, Chromatogr, 15, 730-740.

[22]   Yagi, K. (1987) Lipid peroxides and human diseases. Chem Phys Lipids, 45, 337-351.

[23]   Ellman, G.L. (1959) Tissue sulfhydryl group. Arch. Bio-chem. Biophys, 82: 70-77.

[24]   Ramos-Martines I.L., Torres A.M. (1985) Glutatione reductase of mantle tissue from sea mussel medulis 1. Рurification and characterization two seasonal enzymatic forms. Biochem. Physiol, 80(213), 355-360.

[25]   Mills, G.C. (1959) The purification and properties of glutathione peroxidase of erythrocytes. J. Biol. Chem, 234 (3), 502-506.

[26]   McIntyre, T.M., Zimmerman, G.A., Prescott, S.M. (1999) Biologically Active Oxidized Phospholipids. J Biol Chem, 274(36), 25189-25192.

[27]   Steenbergen, R., Nanowski, T.S., Nelson, R. et al. (2006) Phospholipid homeostasis in phosphatidylserine syn-thase-2-deficient mice. Bioch. Et Biophys. Acta. 1761(3), 313-323.

[28]   Hulbert, A.J., Turner, N., Storlien, L.H. et al. (2005) Dietary fats and membrane function: implications for metabolism and disease. Biol. Rev. Camb. Philos. Soc, 80(1), 155-169.

[29]   Simopoulos, A.P. (2002) The importance of the ratio of omega-6/omega-3 essential fatty acids. Biomed Phar-macother, 56, 365-79.

[30]   Harris, W.S., Poston, W.C., Haddock, C.K. (2007) Tissue n-3 and n-6 fatty acids and risk for coronary heart disease events. Atherosclerosis, 193, 1-10.

[31]   Zhou L., Nilsson А. (2001) Sources of eicosanoid pre-cursor fatty acid pools in tissues. J. Lipid Res, 42, 1521- 1542.

[32]   Novgorodtseva, T.P., Karaman, Yu.K., Antoniuk, M.V., Zhukova, N.V. (2009) The role of free and esterified fatty acids in pathogenesis of metabolic syndrome. Klin Med, 87(5), 33-7.

 
 
Top