Molecular Characterization Using Microsatellites of Bambara Nut (Vigna subterranea [L] Verdcourt) Landraces Cultivated in Burkina Faso

Diane Judicaëlle  Kambou; Nerbéwendé  Sawadogo; Mahamadou  Sawadogo; Abdou Kader  Congo; Wend-Pagnangdé Marie Serge Félicien  Zida; Mahama  Ouedraogo; Hervé  Nandkangre; Adjima  Ouoba; N’Golo Moussa  Konate

doi:10.4236/as.2021.1210072

Diane Judicaëlle Kambou^1,2^*, Hervé Nandkangre³, Adjima Ouoba⁴, Abdou Kader Congo², N’Golo Moussa Konate⁵, Wend-Pagnangdé Marie Serge Félicien Zida², Nerbéwendé Sawadogo¹, Mahamadou Sawadogo¹, Mahama Ouedraogo²

Abstract: Voandzou is a seed legume cultivated in Burkina Faso with significant nutritional potential. The objective of this study was to assess the genetic diversity of Bambara nut cultivated in Burkina Faso using microsatellite markers. For the study, fifteen SSRs markers were used for molecular characterization of 90 Bambara nut landraces from three agro-climatic zones of Burkina Faso. All markers were 100% polymorphic with an average value of 4.81 for effective alleles. The polymorphism information content (PIC) ranged from 0.654 to 0.867 with a mean of 0.775. Dendrogram classified the accessions in four mixed groups independently of the three agro-climatic zones. This distribution is consistent with the results on the agro-morphological characterization of the same landraces. This information was served as a basic model for breeding and conservation programs of V. subterranea in Burkina Faso.

Keywords: Bambara Nut, Landraces, Genetics Diversity, SSR Markers

Cite this paper: Kambou, D. , Nandkangre, H. , Ouoba, A. , Congo, A. , Konate, N. , Zida, W. , Sawadogo, N. , Sawadogo, M. and Ouedraogo, M. (2021) Molecular Characterization Using Microsatellites of Bambara Nut (Vigna subterranea [L] Verdcourt) Landraces Cultivated in Burkina Faso. Agricultural Sciences, 12, 1119-1128. doi: 10.4236/as.2021.1210072.

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