Back
 AS  Vol.12 No.6 , June 2021
Sterilization of Seed Carthamus tinctorius (Safflower) Plant and Investigation of the Effectiveness of the Sterilizants
Abstract: Plant tissue culture studies are one of the pretreatments carried out to increase crop yield by preventing germination in plant seeds. In this research, repeated plant tissue culture studies were conducted with sterilizers specific to safflower seed, which will increase production efficiency but do not cause genetic polymorphism and corrosion in endosperm with 3N chromosomes. Corrosives were used by dilution, and this did not damage the 3N chromosome endosperm, targeting the protein walls of microorganisms on the seed surface without eroding the seed surface, thereby providing biological sterilization. Besides, because it does not contain heavy metals, it did not cause polymorphism, that is, a mutation in the genetic sequence of the seed. Moreover, the environment and the equipment were sterilized with 2 - 3 repetitions, sterilizer treatment, planting, and germination operations were performed in a sterile environment this, in turn, allowed an isolated assessment of the yield of solution G.
Cite this paper: Korkmaz, P. , Demir, H. and Tezcan, E. (2021) Sterilization of Seed Carthamus tinctorius (Safflower) Plant and Investigation of the Effectiveness of the Sterilizants. Agricultural Sciences, 12, 603-619. doi: 10.4236/as.2021.126039.
References

[1]   Gautam, S., Bhagyawant, S.S. and Srivastava, N. (2014) Detailed Study on Therapeutic Properties, Uses and Pharmacological Applications of Safflower (Carthamus tinctorius L.). International Journal of Ayurveda and Pharma Research, 2, 5-16.

[2]   Agricultural Marketing Resource Center (2017) Safflower.
https://www.agmrc.org/commodities-products/grains-oilseeds/safflower

[3]   Sonneville, K. (2013) Manual of Pediatric Nutrition. 5th Edition, Kindle Edition, People’s Medical Publishing House.

[4]   Cox, C., Sutherland, W., Mann, J., De Jong, S., Chisholm, A. and Skeaff, M. (1998) Effects of Dietary Coconut Oil, Butter and Safflower Oil on Plasma Lipids, Lipoproteins and Lathosterol Levels. European Journal of Clinical Nutrition, 52, 650-654.
https://doi.org/10.1038/sj.ejcn.1600621

[5]   Han, X., Cheng, L., Zhang, R. and Bi, J. (2009). Extraction of Safflower Seed Oil by Supercritical CO2. Journal of Food Engineering, 92, 370-376.
https://doi.org/10.1016/j.jfoodeng.2008.12.002

[6]   Food and Agriculture Organization of the United Nations. World Production of Safflower Seeds in 2016.
http://www.fao.org/faostat/en/#search/safflower

[7]   United Nations Food and Agriculture Organization, Statistics Division (FAOSTAT) (2017) The State of Food Security and Nutrition in the Word 2020.

[8]   Ilkılıç, C., Aydın, S., Behcet, R. and Aydin, H. (2011) Biodiesel from Safflower Oil and Its Application in a Diesel Engine. Fuel Processing Technology, 92, 356-362.
https://doi.org/10.1016/j.fuproc.2010.09.028

[9]   Yakar, Y., Tekel, I.Y., Duru, M., Danahaliloglu, H. and Bucak, S. (2014) The Effect of Feeding with Safflower Seed Added Mixed Feed on the Fatty Acid Composition in Eggs. Ziraat Fakultesi Dergisi, Mustafa Kemal University, 19, 44-55.

[10]   Ahmad, M., Nangyal, H., Imran, M. and Ullah, F. (2016) Optimization of Protocol for Surface Sterilization and Callus Induction for Three Rice Varieties. American-Eurasian Journal of Agricultural & Environmental Sciences, 16, 357-361.

[11]   Bakhsh, A., Anayol, E., Sancak, C. and Ozcan, S. (2016) An Efficient and Cost Effective Sterilizing Method with Least Microbial Contamination and Maximum Germination Ratio for in Vitro Cotton (Gossypium hirsutum L.) Culture. Journal of Plant and Animal Sciences, 26, 868-873.

[12]   Birecikli, A.H., Akbas, F. (2018). Effect of Auxin and Cytokinins on Micropropagation and Rooting of in vitro Shoot Tips of Safflower (Carthamus tinctorius L.) cv. “Balci”. Yüzüncü Yil Üniversitesi Journal of Agricultural Sciences, 28, 438-443.

[13]   Tezcan, E. and Atıcı, O.G. (2016) Development of a New Technology for Delignification Process. PhD Thesis, Istanbul Technical University, Istanbul.

[14]   Song, H., Ko, K., Oh, L. and Lee, B. (2006) Fabrication of Silver Nanoparticles and Their Antimicrobial Mechanisms. European Cells & Materials, 11, Article No. 58.

[15]   Turkish Public Health Institution (2015) Working Procedures and Principles of Biocidal Product Analysis Laboratories. T.C. Ministry of Health, Istanbul,Turkey.

[16]   Ministry of Agriculture and Forestry (2010) TS EN 1276. Kimyasal dezenfektanlar ve antiseptikler-gıda sanayi, ev ve kuruluşlarda kullanılan kimyasal dezenfektanlar ve antiseptiklerin bakterisidal aktivitelerinin degerlendirilmesi için nicel süspansiyon deneyi-deney yöntemi ve özellikler. Turkish Standardization Institute, Ankara.

[17]   Ministry of Agriculture and Forestry (2016) TS EN 13727+A2. Kimyasal dezenfektanlar ve antiseptikler-nicel suspansiyon deneyi-Tıbbi alanda bakteri oldurme etkinliğinin degerlendirilmesi için-Deney yöntemi ve gerekler. Turkish Standardization Institute, Ankara.

[18]   Moreira, C.F., Cassini-Vieira, P., da Silva, M.F. and Barcelos, L. (2015) Skin Wound Healing Model-Excisional Wounding and Assessment of Lesion Area. Bio-Protocol, 5, Article No. e1661.
https://doi.org/10.21769/BioProtoc.1661

[19]   Sutton, S. (2006) Measurement of Cell Concentration in Suspension by Optical Density. Pharmaceutical Microbiology Forum Newsletter, 12, 3-13.

[20]   Orlikowska, T.K. and Dyer, W.E. (1993) In Vitro Regeneration and Multiplication of Safflower (Carthamus tinctorius L.). Plant Science, 93, 151-157.
https://doi.org/10.1016/0168-9452(93)90044-Z

[21]   Sánchez, E., Rivas Morales, C., Castillo, S., Leos-Rivas, C., Garcia-Becerra, L. And Ortiz Martinez, D.M. (2016) Antibacterial and Antibiofilm Activity of Methanolic Plant Extracts against Nosocomial Microorganisms. Evidence-Based Complementary and Alternative Medicine, 2016, Article ID: 1572697.
https://doi.org/10.1155/2016/1572697

[22]   Baba, A., Hasezawa, S. and Syōno, K. (1986) Cultivation of Rice Protoplasts and Their Transformation Mediated by Agrobacterium Spheroplasts. Plant and Cell Physiology, 27, 463-471.
https://doi.org/10.1093/oxfordjournals.pcp.a077122

[23]   Lussi, A. (1996) Autoclave. U.S. Patent No. 5535141.

[24]   Thorpe, T.A. (1990) The Current Status of Plant Tissue Culture. Developments in Crop Science, 19, 1-33.
https://doi.org/10.1016/B978-0-444-88883-9.50005-4

[25]   Vasil, I.K. and Thorpe, T.A. (eds.) (1994) Plant Cell and Tissue Culture. Kluwer Academic, Dordrecht.
https://doi.org/10.1007/978-94-017-2681-8

[26]   Debergh, P.C. and Zimmerman, R.H. (Eds.) (1991) Micropropagatio/1. Kluwer Academic, Dordrecht.

[27]   Gomi, M., Osaki, Y., Mori, M. and Sakagami, Y. (2012) Synergistic Bactericidal Effects of a Sublethal Concentration of Didecyldimethylammonium Chloride (DDAC) and Low Concentrations of Nonionic Surfactants against Staphylococcus aureus. Biocontrol Science, 17, 175-181.
https://doi.org/10.4265/bio.17.175

[28]   National Center for Biotechnology Information (2016) Didecyldimethylammonium Chloride. Household Products Database. US Department of Health and Human Services, Washington DC.

 
 
Top