JACEN  Vol.10 No.1 , February 2021
Effects of Light-Emitting Diode (LED) Red and Blue Light on the Growth and Photosynthetic Characteristics of Momordica charantia L.
Abstract: With andromonoecious Momordica charantia L. (bitter gourd) as material, three light qualities (50 μmol·m-2·s-1) including white LED light (WL), blue monochromatic light (B, 465 nm), and red monochromatic light (R, 650 nm) were carried out to investigate their effects on seed germination, physiological and biochemical parameters, sex differentiation and photosynthetic characteristics of bitter gourd. The results showed that compared to the WL treatment, the R treatment significantly promoted seed germination, seedling height elongation and soluble sugar content, the B treatment significantly increased seedling stem diameter, reducing sugar content and soluble protein content, the R and B treatments both significantly reduced sucrose content, but their POD activity showed no significant difference. Compared with the R treatment, the B treatment significantly increased the total female flower number and female flower nod ratio in 30 nods of main stems. The study of photosynthetic characteristics found that the R and B treatments could effectively increase the stomatal conductance (GS) of leaves, significantly improved the net photosynthetic rate (Pn) compared to the WL treatment, and the effect of the B treatment was better. Compared to the R and WL treatments, the B treatment increased the maximum photosynthetic rate (Pmax), apparent quantum efficiency (AQE) and light saturation point (LSP), and reduced the dark respiration rate (Rd) and light compensation point (LCP) of the leaves. Fit light response curves showed that the adaptability and utilization of weak light in bitter gourd were middle or below, but it showed higher adaptability and utilization of strong light. Thus, it suggests that Momordica charantia is a typical sun plan with lower Rd. In summary, it is concluded that blue light has a positive effect on the seed germination, seedling growth, sex differentiation and improving the photosynthetic performance, and this will lay the foundation for artificially regulating optimum photosynthesis using specific LEDs wavelength, and help to elucidate the relationship how light quality influences the sex differentiation of plant.
Cite this paper: Wang, G. , Chen, Y. , Fan, H. and Huang, P. (2021) Effects of Light-Emitting Diode (LED) Red and Blue Light on the Growth and Photosynthetic Characteristics of Momordica charantia L.. Journal of Agricultural Chemistry and Environment, 10, 1-15. doi: 10.4236/jacen.2021.101001.

[1]   Xu, K., Guo, Y.P., Zhang, S.L., Zhang, L.C. and Zhang, L.X. (2005) Effect of Light Quality on Photosynthesis and Chlorophyll Fluorescence in Strawberry Leaves. Scientia Agricultura Sinica, 2, 369-375.

[2]   Alfred, B. (1998) Photoreceptors of Higher Plants. Planta, 206, 479-492.

[3]   Smith, M.A., Palta, J.P. and McCown, B.H. (1986) Comparative Anatomy and Physiology of Microcultured, Seedling, and Greenhouse-Grown Asian While Birch. Journal of the American Society for Horticultrual Science, 111, 437-442.

[4]   Chory, J., Chatterjee, M., Cook, R.K., Elich, T., Fankhauser, C., Li, J., Nagpal, P., Ne, M., Pepper, A., Poole, D., Reed, J. and Vitart, V. (1996) From Seed Germination to Flowering, Light Controls Plant Development via the Pigment Phytochrome. Physical Sciences Social Sciences Biological Sciences USA, 93, 12066-12071.

[5]   Chory, J. (1997) Light Modulation of Vegetative Development. Plant Cell, 9, 1225-1234.

[6]   Liscum, E. and Hangarter, R.P. (1994) Mutational Analysis of Blue-Light Sensing in Arabidopsis. Plant Cell Environment, 17, 639-648.

[7]   Short, T.W. and Briggs, W.R. (1994) The Transduction of Blue Light Signals in Higher Plants. Annual Review of Plant Biology, 45, 143-171.

[8]   Jenkins, G.I., Christie, J.M., Fuglevand, G., Long, J.C. and Jackson, J.A. (1995) Plant Responses to UV and Blue Light: Biochemical and Genetic Approaches. Plant Science, 112, 117-138.

[9]   Briggs, W.R. and Liscum, E. (1997) Blue Light-Activated Signal Transduction in Higher Plants. In: Aducci, P., Ed., Signal Transduction in Plants, Birkhaè User, Basel, 107-135.

[10]   Savvides, A., Fanourakis, D. and van Ieperen, W. (2012) Co-Ordination of Hydraulic and Stomatal Conductances across Light Qualities in Cucumber Leaves. Journal of Experimental Botany, 63, 1135-1143.

[11]   Miao, Y.X., Wang, X.Z., Gao, L.H., Chen, Q.Y. and Qu, M. (2016) Blue Light Is More Essential than Red Light for Maintaining the Activities of Photosystem II and I and Photosynthetic Electron Transport Capacity in Cucumber Leaves. Journal of Integrative Agriculture, 15, 87-100.

[12]   Raman, A. and Lau, C. (1996) Anti-Diabetic Properties and Phytochemistry of Momordica charantia L. (Cucurbitaceae). Phytomedicine, 2, 349-362.

[13]   Kosova, K., Vitamvas, P., Prasil, I.T. and Renaut, J. (2012) Plant Proteome Changes under Abiotic Stress-Contribution of Proteomics Studies to Understanding Plant Stress Response. Journal of Proteomics, 74, 1301-1322.

[14]   Bula, R., Morrow, R., Tibbitts, T., Barta, D., Ignatius, R. and Martin, T. (1991) Light-Emitting Diodes as a Radiation Source for Plants. HortScience, 26, 203-205.

[15]   Li, H.S. (2007) Principle and Technology of Plant Physiological Biochemical Experiment. 2nd Edition, High Education Press, Beijing, 145-148.

[16]   Liu, Y.F., Xiao, L.T., Tong, J.H. and Li, X.B. (2005) Primary Application on the Non-Rectangular Hyperbola Model for Photosynthetic Light-Response Curve. Chinese Agricultural Science Bulletin, 21, 76-79.

[17]   Farqubar, G.D., Caemmerer, S.V. and Berry, J.A. (1980) A Biochemical Model of Photosynthetic CO2 Assimilation in Leaves of C3 Species. Planta, 149, 78-90.

[18]   Qiu, G.W. (1992) Efficiency of Plant Photosynthesis. In: Tang, Z.C., Ed., Plant Physiology and Molecular Biology, Beijing Science Press, Beijing, 236-244.

[19]   Zhang, R.H., Xu, K. and Dong, C.X. (2008) Effect of Light Quality on Photosynthetic Characteristics of Ginger Leaves. Scientia Agricultura Sinica, 41, 3722-3727.

[20]   Melis, A. (1999) Photosystem-II Damage and Repair Cycle in Chloroplasts: What Modulates the Rate of Photodamage in Vivo? Trends Plant Science, 4, 130-135.

[21]   Rajagopal, S., Murthy, S. and Mohanty, P. (2000) Effect of Ultraviolet-B Radiation on Intact Cells of the Cyanobacterium Spirulina platensis: Characterization of the Alterations in the Thylakoid Membranes. Journal of Photochemistry and Photobiology B, 54, 61-66.

[22]   Saebo, A., Krekling, T. and Appelgren, M. (1995) Light Quality Affects Photosynthesis and Leaf Anatomy of Birch Plantlets in Vitro. Plant Cell Tissue and Organ Culture, 41, 177-185.

[23]   Hogewoning, S.W., Trouwborst, G., Maljaars, H., Poorter, H., van Ieperen, W. and Harbinson, J. (2010) Blue Light Dose-Responses of Leaf Photosynthesis, Morphology, and Chemical Composition of Cucumis sativus Grown under Different Combinations of Red and Blue Light. Journal of Experimental Botany, 61, 3107-3117.

[24]   Cope, K.R., Snowden, M.C. and Bugbee, B. (2014) Photobiological Interactions of Blue Light and Photosynthetic Photon Flux: Effects of Monochromatic and Broad-Spectrum Light Sources. Photochemistry and Photobiology, 90, 574-584.

[25]   O’Carrigan, A., Babla, M., Wang, F., Liu, X., Mak, M., Thomas, R., Bellotti, B. and Chen, Z.H. (2014) Analysis of Gas Exchange, Stomatal Behaviour and Micronutrients Uncovers Dynamic Response and Adaptation of Tomato Plants to Monochromatic Light Treatments. Plant Physiology and Biochemistry, 82, 105-115.

[26]   Matsuda, R., Ohashi-Kaneko, K., Fujiwara, K. and Kurata, K. (2008) Effects of Blue Light Deficiency on Acclimation of Light Energy Partitioning in PSII and CO2 Assimilation Capacity to High Irradiance in Spinach Leaves. Plant Cell Physiology, 49, 664-670.

[27]   Hogewoning, S.W., Wientjes, E., Douwstra, P., Trouwborst, G., van Ieperen, W., Croce, R. and Harbinson, J. (2012) Photosynthetic Quantum Yield Dynamics: From Photosystems to Leaves. Plant Cell, 24, 1921-1935.

[28]   Lin, C. and Todo, T. (2005) The Cryptochromes. Genome Biology, 6, 220.

[29]   Shimazaki, K.I., Doi, M.S., Assmann, M. and Kinoshita, T. (2007) Light Regulation of Stomatal Movement. Annual Review of Plant Biology, 58, 219-247.

[30]   Dumont, J., Spicher, F., Montpied, P., Dizengremel, P., Jolivet, Y. and Thiec, D.L. (2013) Effects of Ozone on Stomatal Responses to Environmental Parameters (Blue Light, Red Light, CO2 and Vapour Pressure Deficit) in Three Populus deltoides x Populus nigra Genotypes. Environmental Pollution, 173, 85-96.

[31]   Boccalandro, H.E., Giordano, C.V., Ploschuk, E.L., Piccoli, P.N., Bottini, R. and Casal, J.J. (2012) Phototropins But Not Cryptochromes Mediate the Blue Light- Specific Promotion of Stomatal Conductance, While Both Enhance Photosynthesis and Transpiration under Full Sunlight. Plant Physiolology, 158, 1475-1484.

[32]   Araújo, W.L., Fernie, A.R. and Nunes-Nesi, A. (2011) Control of Stomatal Aperture. A Renaissance of the Old Guard. Plant Signal Behavior, 6, 1305-1311.

[33]   Busch, F.A. (2014) Opinion: The Red-Light Response of Stomatal Movement Is Sensed by the Redox State of the Photosynthetic Electron Transport Chain. Photosynthesis Research, 119, 131-140.

[34]   Wang, Y. and Folta, K.M. (2013) Contributions of Green Light to Plant Growth and Development. American Journal of Botany, 100, 70-78.

[35]   Aasamaa, K. and Aphaloa, P.J. (2016) Effect of Vegetational Shade and Its Components on Stomatal Responses to Red, Blue and Green Light in Two Deciduous Tree Species with Different Shade Tolerance. Environmental and Experimental Botany, 121, 94-101.