Back
 JBBS  Vol.10 No.10 , October 2020
A Study on the Association between Patients with Post-Traumatic Stress Disorder in Li and Han Ethnic Groups in Hainan Province and DNA Methylation of Brain-Derived Neurotrophic Factor Genes
Abstract: Objective: To explore the pathogenesis of PTSD in the brain-derived neurotrophic factor (BDNF) gene methylation of patients with posttraumatic stress disorder (Posttraumatic Stress Disorder, PTSD) in Hainan Province, the relationship between the influence of BDNF gene methylation and the influence of PTSD. Methods: A case-control study method was adopted, strictly in accordance with DSM-IV and PTSD diagnosis, and 150 Li PTSD patients matched with gender and age of 300 Han PTSD patients were selected as the research objects. The peripheral venous whole blood of the subjects was drawn, genomic DNA was extracted, modified with bisulfite, and directly sequenced to quantitatively detect the methylation status of the CpG island in the promoter region of brain-derived neurotrophic factor (BDNF). Results: The results showed that the methylation levels of CPGl, CPG2, CPG3, CPG4, CPG5, CPG6, CPG7, CPG9, CPGl2, CPGl3, CPGl4, CPGl5, CPGl6, CPGl7, and CPGl8 in THE BDNF promoter were significantly different between the HAN PTSD group and the Li PTSD group (P < 0.001). Conclusion: It is suggested that CPG methylation in the promoter region of BDNF gene is closely related to patients with PTSD. There is a statistical difference in the level of CpG methylation in the promoter region of BDNF gene in PTSD between Li and Han ethnic groups in Hainan Province. CpG methylation in the promoter region of BDNF gene may be used as a biomarker for the diagnosis of PTSD.
Cite this paper: Wang, X. , Gao, Y. , Guo, M. and Guo, J. (2020) A Study on the Association between Patients with Post-Traumatic Stress Disorder in Li and Han Ethnic Groups in Hainan Province and DNA Methylation of Brain-Derived Neurotrophic Factor Genes. Journal of Behavioral and Brain Science, 10, 400-409. doi: 10.4236/jbbs.2020.1010025.
References

[1]   Kelly, K., McCarron, M.J., Reinhard, K.J., Bloeser, C.M., Mahan, H.K., Kang, et al. (2014) PTSD Diagnoses among Iraq and Afghanistan Veterans: Comparison of Administrative Data to Chart Review. Journal of Traumatic Stress, 5, 626-629.
https://doi.org/10.1002/jts.21961

[2]   Svenaeus, F. (2013) Diagnosing Mental Disorders and Saving the Normal. Medicine, Health Care and Philosophy, 17, 241-244.

[3]   Lin, X.B., Cen, J.R., Li, Z.Q., et al. (2014) A Survey of 347 Couples with Genetic Risk of Intermediate or Heavy Thalassemia in Rural Hainan Province. Hainan Studies, 22, 3397-3401.

[4]   Schwarzer, R., Cone, J.E., Li, J., Bowler, R.M., et al. (2016) A PTSD Symptoms Trajectory Mediates between Exposure Levels and Emotional Support in Police Responders to 9/11: A Growth Curve Analysis. BMC Psychiatry, 16, Article No. 201.
https://doi.org/10.1186/s12888-016-0907-5

[5]   Li, L.J. and Yu, X. (2013) Guide to the Prevention and Treatment of Post-Traumatic Stress Disorder. Chinese Journal of Neurology, 46, 196-198.

[6]   Joy, D.M., et al. (2013) Genomic and Epigenomic Insights into Nutrition and Brain Disorders. Nutrients, 5, 887-914.
https://doi.org/10.3390/nu5030887

[7]   Notaras, M., Hill, R., Van, d.B.M., et al. (2015) The BDNF Gene Val66Met Polymorphism as a Modifier of Psychiatric Disorder Susceptibility: Progress and Controversy. Molecular Psychiatry, 20, 916-930.

[8]   Han, E.J., Kim, Y.K., Hwang, J., Kim, S.H., Lee, H.J., et al. (2015) Evidence for Association between the Brain-Derived Neurotrophic Factor Gene and Panic Disorder: A Novel Haplotype Analysis. Psychiatry Investigation, 12, 112-117.
https://doi.org/10.4306/pi.2015.12.1.112

[9]   Li, Y., Fu, Q. and Liu, A.Z. (2015) Other Neurobiological Related Genes Research Progress in Post-Traumatic Stress Disorder. Chinese Journal of Trauma, 31, 183-186.

[10]   Hemmings, S., Martin, L., Klopper, M., et al. (2013) BDNF Val66Met and DRD2 TaqlA Polymorphisms Interact to Influence PTSD Symptom Severity: A Preliminary Investigation in a South African Population. Progress in Neuro-Psychopharmacology & Biological Psychiatry, 40, 273.
https://doi.org/10.1016/j.pnpbp.2012.10.011

[11]   Valente, N.L., Vallada, H., Cordeiro, Q., et al. (2011) Candidate-Gene Approach in Posttraumatic Stress Disorder after Urban Violence: Association Analysis of the Genes Encoding Serotonin Transporter, Dopamine Transporter, and BDNF. Journal of Molecular Neuroscience, 44, 59-67.
https://doi.org/10.1007/s12031-011-9513-7

[12]   Strauss, J.S., Khare, T., De Luca, V., et al. (2013) Quantitative Leukocyte BDNF Promoter Methylation Analysis in Bipolar Disorder. International Journal of Bipolar Disorders, 1, Article No. 28.
https://doi.org/10.1186/2194-7511-1-28

[13]   Dell, O.B., D’Addario, C., Carlotta, P.M., et al. (2014) Epigenetic Modulation of BDNF Gene: Differences in DNA Methylation between Unipolar and Bipolar Patients. Journal of Affective Disorders, 166, 330-333.
https://doi.org/10.1016/j.jad.2014.05.020

[14]   TaIiaz, D. and Stall, N. (2010) Knockdown of Brain-Derived Neurotrophic Factor in Specific Brain Sites Precipitates Behaviors Associated with Depression and Reduces Neurogenesis. Molecular Psychiatry, 15, 80-92.
https://doi.org/10.1038/mp.2009.67

[15]   Fuchikami, M., Morinobu, S., Segawa, M., et al. (2011) DNA Methylatio Profiles of the BDNF Gene as a Potent Diagnostic Biomarker in Major Depression. PLoS ONE, 6, 881-888.
https://doi.org/10.1371/journal.pone.0023881

[16]   Okada, S., Morinobu, S., Fuchikami, M., et al. (2014) The Potential of SLC6A4 Gene Methylation Analysis for the Diagnosis and Treatment of Major Depression. Journal of Psychiatric Research, 53, 47-53.
https://doi.org/10.1016/j.jpsychires.2014.02.002

[17]   Karege, F., Bondolfi, G., Gervasoni, N., et al. (2005) Low Brain-Derived Neurotrophic Factor (BDNF) Levels in Serum of Depressed Patients Probably Results from Lowered Platelet BDNF Release Unrelated to Platelet Reactivity. Biological Psychiatry, 57, 1068-1072.
https://doi.org/10.1016/j.biopsych.2005.01.008

[18]   Carlberg, L., Scheibelrciter, J., Hassler, M.R., et al. (2014) Brain-Derived Neurotrophic Factor (BDNF)-Epigenetic Regulation in Unipolar and Bipolar Affective Disorder. Journal of Affective Disorders, 168, 399-406.
https://doi.org/10.1016/j.jad.2014.07.022

[19]   Smoller, J.W. and Finn, C.T. (2003) Family, Twin and Adoption Studies of Bipolar Disorder. American Journal of Medical Genetics. Part C: Seminars in Medical Genetics, 123, 48-58.
https://doi.org/10.1002/ajmg.c.20013

[20]   Xing, B. and Liu, P. (2014) Effect of Microinjecting of 5-Aza-2-deoxycytidine into Ventrolateral Orbital Cortex on Depressive-Like Behavior in Rats. Neuroscience Letters, 574, 11-14.
https://doi.org/10.1016/j.neulet.2014.04.050

[21]   Sun, T., Li, G.X. and Gao, S.G. (2016) Research Progress of Brain-Derived Neurotrophic Factor Gene Methylation in Depression. Clinical Meta-Analysis, 31, 1151-1154.

[22]   Zhou, J.-X., Ding, M., Cui, D., Zhang, L.L., Zhou, Z.H., Qin, S.-D., Liao, R.Q., Wang, Y., He, F.S., Yang, F. and Wang, M.B. (2017) Preliminary Study on the Relationship between Brain-Derived Neurotrophic Factor Promoter Methylation and Autism Spectrum Disorders. Chinese Journal of Neuropsychiatric Disorders, 43, 98-102.

[23]   Li, L., Zhang, X.R., Zhang, Z.X. and Wang, Y.X. (2012) Effects of Escitalopram on Exon Expression and DNA Methylation of Hippocampal Brain-Derived Neurotrophic Factor Gene in Chronic Stress Rats. Chinese Journal of Psychiatry, 54, 232-236.

 
 
Top