The immoderate utilization of multiple antibiotics conducted to the extensive diffusion of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in numerous environment matrices like water, sludge , soil , sediment, etc.    . Such a resistance decreases the effectiveness of antibiotics in dealing with infectious diseases, which provokes more than 23,000 deaths per year in the U.S., nearly 25,000 deaths per year in Europe, and even more in less-developed countries   . It turned into a worldwide problem for human and animal health . The antibiotic resistance could be diffused by sharing ARGs among microorganisms through horizontal gene transfer (HGT) . Plasmids, integrons, and transposons are the mobile genetic elements frequently implied in the ARG sharing phenomena . In nature, ARGs could remain even after the bacteria are dead . Both intracellular and extracellular ARGs are all set to adapt to novel hosts . The effluents from wastewater treatment plants (WWTPs) and livestock production, frequently with elevated levels of ARGs, are regarded to be important sources of ARB and ARGs in nature   .
The traditional disinfection techniques (e.g., chlorination   , UV irradiation   , and ozonation ) in water and wastewater treatment    have been found efficient in demobilizing ARB effectively   . Nevertheless, most of the ARGs endured even when the ARB are fully demobilized during the disinfection techniques   . Rather than all of the time, the disinfection technologies could demolish bacterial deoxyribonucleic acid (DNA) or the cellular structure   ; however, ARGs could remain in the cell debris and the extracellular ARGs are still causing continuing danger   . Lately, techniques for eliminating intracellular ARGs have been to a great degree studied comprising enhanced disinfection   , constructed wetland   , and advanced oxidation process (AOPs)    . Zhang et al.  illustrated a positive relationship between the demobilization of ARGs and the Cl2 injection and residence period; further, they proved that consecutive UV/chlorination    could ameliorate demobilization considerably. To reduce both ARB and ARGs efficiently, Oh et al.  found that an injection of Cl2 as high as 30 mg/L or an injection of 3 mg/L O3 is needed. As the dose of UV irradiation augmented, the abundance of ARGs reduced exponentially . Elevated doses of UV irradiation (>10 mJ/cm2) reduced ARB and ARGs greatly but considerably augmented the frequency of ARGs transfer together for the higher pressure . Constructed wetlands, particularly those possessing a surface flow pattern, have demonstrated acceptable ARGs removal performances; however, the danger of augmented ARGs transfer still endured   . For that reason, novel substitutional methods with elevated reduction performance and low hazard of ARGs transfer are highly required   . Lately, AOPs (like Fenton reaction , TiO2 photocatalysis, and UV/H2O2 ) have demonstrated elevated capacity to demobilize ARB and ARGs   . In the Fenton treatment and UV/H2O2 process, the hydroxyl radicals could reduce ARGs efficaciously (2.3 - 3.8 logs of decrease) and the Fenton treatment achieved better than UV/H2O2 process  . Guo et al.  proved that photocatalysis by TiO2 has the potential to decrease ARB by 4.5 - 5.8 logs and ARGs by 4.7 - 5.8 logs. For their elevated reduction performance, AOPs are encouraging manners for decreasing ARB and ARGs   . However, there are still some gaps in expertise   . As an illustration, even if electrochemical (EC) disinfection has been largely employed in killing different bacteria   , viruses   , and microalgae   , its capability in reducing ARGs has not been explored pointedly   . Moreover, all the previous researches have concentrated on eliminating intracellular ARGs; however, there is no data about reducing extracellular ARGs performances.
This work discusses the inactivation of ARB and ARGs by EC oxidation/electro-Fenton process. Further, it compares electro-Fenton and photo Fenton like process UV-C/H2O2/IDS-Cu method with other AOPs techniques. A special focus is accorded to decreased Klebsiella michiganensis strain LH-2 viability and corresponding ARG abundance in bioelectrochemical reactors (BERs). The fate of ARGs during bioelectrochemical treatment of high-salinity pharmaceutical wastewater is discussed. Finally, dares and prospects for bioelectrochemical systems (BESs) are reviewed.
2. Inactivation of Antibiotic-Resistant Bacteria (ARB) and Antibiotic Resistance Genes (ARGs) by Electrochemical (EC) Oxidation/Electro-Fenton Process
Recently, Chen et al.  assessed the capability of EC oxidation and electro-Fenton method as substitutional treatment processes for demobilizing ARB and ARGs in both intracellular and extracellular forms. They proved that EC oxidation technique was efficacious in dealing with chosen ARB; however, not in treating intracellular ARGs or extracellular ARGs. The electro-Fenton method was more performant in eliminating both intracellular and extracellular ARGs. Reducing efficacy following 120 min of electro-Fenton application under 21.42 mA/cm2 was 3.8 logs for intracellular tetA, 4.1 logs for intracellular ampC, 5.2 logs for extracellular tetA and 4.8 logs for extracellular ampC, respectively in the occurrence of 1.0 mmol/L Fe2+ (Figure 1). They concluded that EC oxidation was a performant disinfection technology for ARB and the electro-Fenton process is an encouraging process for eliminating both intracellular and extracellular ARGs in wastewater.
3. Comparing Electro-Fenton and Photo Fenton Like Process UV-C/H2O2/IDS-Cu Method with Other AOPs Techniques
AOPs are extremely performant in demobilizing ARGs more than traditional disinfection techniques (e.g., chlorination, UV, and ozonation). Zhang et al. 
Figure 1. Effect of initial pH on the removal of ARGs after 120 min of electro-Fenton treatment (current density: 21.42 mA/cm2; Fe2+ concentration: 1.0 mmol/L) .
illustrated that the maximum reduction of ARGs by the UV/H2O2 method and the Fenton technique was 2.8 - 3.5 logs and 2.6 - 3.8 logs, respectively. Whilst Guo et al.  noted that a reduction of 5.2 logs intracellular mecA, 3.3 logs extracellular mecA, 4.4 logs intracellular ampC and 2.6 logs extracellular ampC were attained by UV/H2O2/TiO2 photocatalysis (Table 1). The electro-Fenton method appears as an encouraging method for eliminating both intracellular and extracellular ARGs in wastewater.
Recently, Di Cesare et al.  juxtaposed the performance of a novel AOP, namely the photo Fenton like process UV-C/H2O2/IDS-Cu, in eliminating determinants of antibiotic resistance and pathogenic bacteria to a consolidated AOP (namely UV-C/H2O2) in a secondary treated municipal wastewater. Tests were realized in both, human pathogens favorable conditions (HPC, in rich medium and 37˚C) and in environmental mimicking conditions (EMC, original wastewater and 20˚C). UV-C/H2O2/IDS-Cu method resulted to be more efficient than the UV-C/H2O2 in demobilizing bacterial cells in the EMC post-treatment regrowth tests. Both AOPs were efficaciously abating potential human pathogenic bacteria and ARGs in the HPC regrowth tests, even if such a tendency cannot be detected in the measurements taken immediately following the disinfection. In comparison with the UV-C/H2O2, the UV-C/H2O2/IDS-Cu technique did not clearly provide considerable amelioration in decreasing the tried parameters in the wastewater effluent. By estimating the findings of the regrowth trials it was however easy to extrapolate more complex tendencies, suggesting opposite performances that are visible only after a few hours. Di Cesare et al. 
Table 1. Juxtaposing electro-Fenton with other AOPs techniques for eliminating ARGs .
presented a detailed discussion of the elimination performance of microbiological/genetic parameters for the UV-C/H2O2/IDS-Cu technique, calling for technical adjustments for this extremely encouraging method. Moreover, Di Cesare et al.  comprehensibly proved the inadequacy of currently applied methodologies in the estimation of specific parameters (e.g. determinants of antibiotic resistance and pathogenic bacteria) in wastewater.
4. Decreased Klebsiella michiganensis Strain LH-2 Viability and Corresponding Antibiotic Resistance Gene (ARG) Abundance in Bioelectrochemical Reactors (BERs)
Researchers demonstrated that the electrolytic stimulation method in a bioelectrochemical reactor (BER) could accelerate the growth of sulfadiazine (SDZ) antibiotic-resistant bacteria (ARB) in nutrient broth medium . Nevertheless, the effect of various medium nutrient richness on the fate of ARB and the relative abundance of their corresponding ARGs in such technique is little known. Precisely, it is not known if the fate of ARB in minimal nutrition simulated wastewater is the same as in nutrient broth under electrolytic stimulation . Thus, Li et al.  compared nutrient broth medium and the simulated wastewater to determine differences in the relative abundance of Klebsiella michiganensis LH-2 ARGs in response to the electrolytic stimulation process, as well as the fate of the strain in simulated wastewater. They obtained lower biomass, specific growth rates, and viable bacterial counts in response to the application of increasing current to simulated wastewater medium (Figure 2). In addition, the percentage of ARB lethality, which was reflected by flow cytometry analysis, augmented with the current in the medium. An important positive correlation of sul genes and intI gene relative abundance versus current was also noted in nutrient broth. Nevertheless, an important negative correlation was noted in simulated wastewater due to the higher metabolic burden, which may have conducted to reduced ARB viability. The reduction in ARGs abundance was responsible for reduced strain tolerance to SDZ in simulated wastewater. Minimal nutrition simulated wastewater may decrease ARB and ARGs propagation in BER.
Figure 2. Effects of different current intensities on changes of K. michiganensis LH-2 resistance characteristic to sulfadiazine. (a) Survival rate of the strain exposed to different concentration sulfadiazine in simulated wastewater medium without glucose after electrolytic stimulation. (b) Sul genes and intI gene relative abundance change of K. michiganensis LH-2 in simulated wastewater medium without glucose after electrolytic stimulation .
5. The Fate of Antibiotic Resistance Genes (ARGs) during Bioelectrochemical Treatment of High-Salinity Pharmaceutical Wastewater
Pharmaceutical wastewaters carrying antibiotics and high salinity could harm conventional biological treatment and conduct to the spread of ARGs   . Bioelectrochemical system (BES) is an encouraging method for treating pharmaceutical wastewater. Nevertheless, the fate of ARGs in BES and their correlations with microbial communities and horizontal genes transfer stay unknown. Guo et al.  examined the response of ARGs to bioelectrochemical treatment of chloramphenicol (CAP) wastewater and their potential hosts below various salinities. Three ARGs encoding efflux pump (cmlA, floR and tetC), one class 1 integron integrase encoding gene (intI1), and sul1 gene (associate with intI1) were followed. Correlation analysis between the microbial community and ARGs showed that the abundances of potential hosts of ARGs were greatly influenced by salinity, which further determined the modification in ARGs abundances below diverse salinities. There were no important correlations between ARGs and intI1, showing that horizontal gene transfer was not related to the considerable modifications in ARGs (Figure 3). Further, the CAP reduction performance was improved under a moderate salinity, attributed to the altered microbial community driven by salinity. Consequently, microbial community shift is the major factor for the changes of ARGs and CAP removal efficiency in BES under different salinities. Guo et al.  suggested novel insights on the mechanisms underlying the change of ARGs in BES treating high-salinity pharmaceutical wastewater.
Figure 3. Quantitative correlation of the bacterial genera and ARGs in the samples collected from BESs under different salinities. The correlation analysis was performed using SPSS software, and the heatmap was drawn by R language. The scale bar shows the Spearman Indices (R). Red represents the positive correlation, and blue represents the negative correlation. An asterisk (*) indicates Significant Correlation (P < 0.05), and two asterisks (**) indicate Extreme Significant Correlation (P < 0.01) .
6. Dares and Prospects for Bioelectrochemical Systems (BESs)
By merging microbial metabolism and EC redox reduction, BESs are adopted as an emerging environment-benign and encouraging handling for emerging contaminants, particularly antibiotics. Yan et al.  discussed the impact of different environmental agents on the BESs’ efficiency, functional microbes, and ARGs. Nevertheless, the present pieces of literature mostly focused on searching functional bacteria but not further to discover the biocatalyst pathway about functional genes. Further, numerous researches were dedicated to examining the elimination potential of conventional BESs for wastewater carrying antibiotics but not participate in developing BESs to deal with antibiotics contaminants concentrated in solid matrixes. Consequently, taking into account the diversity of antibiotic contaminants and the complexity of realistic pollutant environments, numerous dares in terms of the amelioration of the elimination capability, a revelation of biocatalyst pathway, development of BESs, and ARGs research stay to be addressed and require more focus (Table 2).
This work discussed the inactivation of ARB and ARGs by EC oxidation/electro-Fenton process and compared electro-Fenton and photo Fenton like process UV-C/H2O2/IDS-Cu method with other AOPs techniques. A special focus is accorded to decreased Klebsiella michiganensis strain LH-2 viability and corresponding ARG abundance in bioelectrochemical reactors and the fate of ARGs during bioelectrochemical treatment of high-salinity pharmaceutical wastewater. Dares and prospects for bioelectrochemical systems (BESs) are suggested. The main points drawn from this work are listed below:
1) The CA and Cu2+ removal ability of a BES was studied and the fate of the ARGs (cmlA, floR, tetC, and sul1) and intI1 was followed , and the bacterial community’s structure when the cathode was exposed to different initial concentrations of Cu2+. The efficiency of the BES for CAP removal was inhibited when Cu2+ and CAP coexisted and the inhibition effect increased with increasing Cu2+ concentration. Further, the various concentrations of Cu2+ dramatically changed the relative abundances of the ARGs and the bacterial community structure in the BES. The shift of the potential host bacteria mainly contributed to the changes in the ARGs (except for sul1).
2) Laboratory-scale EC disinfection tests were performed to examine its reduction performance for 23 ARGs that confer against eight classes of antibiotics and its effects on the antibiotic resistance of surviving bacteria . EC treatments were realized at varying current densities (D treatment) and with different reaction times (T treatment). Prolonged electrolysis conducted to a higher demobilization rate than an augmented current density, while the former was less efficient in the removal of ARGs. As an illustration, the demobilization ratios for the T20 and D80 treatments were both >99%, while the decrease in the relative
Table 2. Dares and prospects for bioelectrochemical systems (BESs) .
abundance of ARGs with D80 (from 0.54 to 4.1) was greater than that with T20 (from 5.4 to 5.2). The detection frequency of bacteria resistant to the tested antibiotics decreased by 9% - 100% after EC treatment. This was mainly attributed to a change in bacterial composition. The proportion of bacteria with high antibiotic resistance frequencies decreased (like Escherichia), while that with low resistance frequencies (such as Acinetobacter and Pseudomonas) increased. Further, fewer multi-antibiotic-resistant bacteria survived EC disinfection, which also contributed to the significant decrease in the frequency of ARB as well as in the multi-antibiotic-resistance indices of wastewater samples (from 0.47 to 0.35) after EC treatment (P < 0.05). In total, EC disinfection not only reduced the relative abundance of ARGs but also impaired the antibiotic resistance of surviving bacteria. Therefore, it might be a promising disinfection method for controlling the dissemination of antibiotic resistance.
3) For removing antibiotics, BES has numerous advantages and disadvantages . The efficient removal of antibiotics occurs mainly due to faster oxidation through co metabolic degradation or direct oxidation by the anode in which the antibiotic is served as a sole electron donor in microbial fuel cells (MFCs). For the microbial electrolysis cells (MECs), a cathode can provide continuous electrons for the reduction of antibiotics. The most abundant phylum in BES is Proteobacteria. Antibiotics and electric current affect the microbial communities and their relative abundances. Antibiotics can be used as the sole carbon source for electricity generation in MFCs, but antibiotics could inhibit the electricity-generating activity of the microbial community. Therefore, the relationship between antibiotics and electricity generation requires further investigation. In addition, a low electric current could promote ARG transfer through vertical gene transfer (VGT) and horizontal gene transfer (HGT) during antibiotic degradation in BES. ARB and ARGs are eliminated with the high electric current. Questions regarding the potential role of BES for antibiotic removal and the reduction of ARGs and ARB are raised   . Further research is needed to elucidate the primary ARG transfer mechanism and to fully understand the advantages of BESs.
The Research Deanship of University of Ha’il, Saudi Arabia, through the Project RG-191190, has funded this research.
 He, P.J., Zhou, Y.Z., Shao, L.M., Huang, J.H., Yang, Z. and Lü, F. (2019) The Discrepant Mobility of Antibiotic Resistant Genes: Evidence from Their Spatial Distribution in Sewage Sludge Flocs. Science of the Total Environment, 697, Article ID: 134176.
 Li, H.N., Li, B.X., Ma, J.L., Ye, J., Guo, P. and Li, L.F. (2018) Fate of Antibiotic-Resistant Bacteria and Antibiotic Resistance Genes in the Electrokinetic Treatment of Antibiotic-Polluted Soil. Chemical Engineering Journal, 337, 584-594. https://doi.org/10.1016/j.cej.2017.12.154
 Chen, L., Zhou, Z., Shen, C.F. and Xu, Y.L. (2020) Inactivation of Antibiotic-Resistant Bacteria and Antibiotic Resistance Genes (ARGs) by Electrochemical Oxidation/Electro-Fenton Process. Water Science & Technology, 81, 2221-2231.
 Auguet, O., Pijuan, M., Borrego, C.M., Rodriguez-Mozaz, S., Triadó-Margarit, X., Giustina, S.V.D. and Gutierrez, O. (2017) Sewers as Potential Reservoirs of Antibiotic Resistance. Science of the Total Environment, 605-606, 1047-1054. https://doi.org/10.1016/j.scitotenv.2017.06.153
 Qiao, M., Ying, G.G., Singer, A.C. and Zhu, Y.Z. (2018) Review of Antibiotic Resistance in China and Its Environment. Environment International, 110, 160-172.
 Cheng, J., Tang, X. and Cui, J. (2019) Effect of Long-Term Manure Slurry Application on the Occurrence of Antibiotic Resistance Genes in Arable Purple Soil (Entisol). Science of the Total Environment, 647, 853-861. https://doi.org/10.1016/j.scitotenv.2018.08.028
 Vikesland, P.J., Pruden, A., Alvarez, P.J.J., Aga, D., Bürgmann, H., Li, X.D., Manaia, C.M., Nambi, I., Wigginton, K., Zhang, T. and Zhu, Y.G. (2017) Towards a Comprehensive Strategy to Mitigate Dissemination of Environmental Sources of Antibiotic Resistance. Environmental Science & Technology, 51, 13061-13069. https://doi.org/10.1021/acs.est.7b03623
 Ghernaout, D. and Elboughdiri, N. (2020) Removing Antibiotic-Resistant Bacteria (ARB) Carrying Genes (ARGs): Challenges and Futuretrends. Open Access Library Journal, 7, e6003.
 Sharma, V.K., Johnson, N., Cizmas, L., Mcdonald, T.J. and Kim, H. (2016) A Review of the Influence of Treatment Strategies on Antibiotic Resistant Bacteria and Antibiotic Resistance Genes. Chemosphere, 150, 702-714. https://doi.org/10.1016/j.chemosphere.2015.12.084
 Dodd, M.C. (2012) Potential Impacts of Disinfection Processes on Elimination and Deactivation of Antibiotic Resistance Genes during Water and Wastewater Treatment. Journal of Environmental Monitoring, 14, 1754-1771. https://doi.org/10.1039/c2em00006g
 Pruden, A. (2014) Balancing Water Sustainability and Public Health Goals in the Face of Growing Concerns about Antibiotic Resistance. Environmental Science & Technology, 48, 5-14.
 Ghernaout, D., Alshammari, Y. and Alghamdi, A. (2018) Improving Energetically Operational Procedures in Wastewater Treatment Plants. International Journal of Advanced and Applied Sciences, 5, 64-72. https://doi.org/10.21833/ijaas.2018.09.010
 Alshammari, Y., Ghernaout, D., Aichouni, M. and Touahmia, M. (2018) Improving Operational Procedures in Riyadh’s (Saudi Arabia) Water Treatment Plants Using Quality Tools. Applied Engineering, 2, 60-71.
 Ghernaout, D., Naceur, M.W. and Aouabed, A. (2011) On the Dependence of Chlorine By-Products Generated Species Formation of the Electrode Material and Applied Charge during Electrochemical Water Treatment. Desalination, 270, 9-22.
 Ghernaout, D., Moulay, S., Ait Messaoudene, N., Aichouni, M., Naceur, M.W. and Boucherit, A. (2014) Coagulation and Chlorination of NOM and Algae in Water Treatment: A Review. International Journal of Environmental Monitoring and Analysis, 2, 23-34.
 Ghernaout, D. and Elboughdiri, N. (2020) UV-C/H2O2 and Sunlight/H2O2 in the Core of the Best Available Technologies for Dealing with Present Dares in Domestic Wastewater Reuse. Open Access Library Journal, 7, e6161. https://doi.org/10.4236/oalib.1106161
 Ghernaout, D. and Elboughdiri, N. (2020) Urgent Proposals for Disinfecting Hospital Wastewaters during COVID-19 Pandemic. Open Access Library Journal, 7, e6373.
 Ghernaout, D. (2013) The Best Available Technology of Water/Wastewater Treatment and Seawater Desalination: Simulation of the Open Sky Seawater Distillation. Green Sustain. Green and Sustainable Chemistry, 3, 68-88. https://doi.org/10.4236/gsc.2013.32012
 Ghernaout, D. and Ghernaout, B. (2010) From Chemical Disinfection to Electrodisinfection: The Obligatory Itinerary? Desalination and Water Treatment, 16, 156-175.
 Ghernaout, D. (2018) Disinfection and DBPs Removal in Drinking Water Treatment: A Perspective for a Green Technology. International Journal of Advanced and Applied Sciences, 5, 108-117. https://doi.org/10.21833/ijaas.2018.02.018
 Ghernaout, D., Aichouni, M. and Touahmia, M. (2019) Mechanistic Insight into Disinfection by Electrocoagulation—A Review. Desalination and Water Treatment, 141, 68-81.
 Czekalski, N., Imminger, S., Salhi, E., Veljkovic, M., Kleffe, K. and Drissner, D. (2016) Inactivation of Antibiotic Resistant Bacteria and Resistance Genes by Ozone: from Laboratory Experiments to Full-Scale Wastewater Treatment, Environmental Science & Technology, 50, 11862-11871.
 Al Arni, S., Amous, J. and Ghernaout, D. (2019) On the Perspective of Applying of a New Method for Wastewater Treatment Technology: Modification of the Third Traditional Stage with Two Units, one by Cultivating Microalgae and Another by Solar Vaporization. International Journal of Environmental Sciences & Natural Resources, 16, Article ID: 555934.
 Ghernaout, D., Elboughdiri, N. and Ghareba, S. (2019) Drinking Water Reuse: One- Step Closer to Overpassing the “Yuck Factor”. Open Access Library Journal, 6, e5895.
 Ghernaout, D. and Elboughdiri, N. (2020) Advanced Oxidation Processes for Wastewater Treatment: Facts and Future Trends. Open Access Library Journal, 7, e6139.
 Ghernaout, D., Elboughdiri, N., Ghareba, S. and Salih, A. (2020) Electrochemical Advanced Oxidation Processes (EAOPs) for Disinfecting Water—Fresh Perspectives. Open Access Library Journal, 7, e6257. https://doi.org/10.4236/oalib.1106257
 Ghernaout, D. (2013) Advanced Oxidation Phenomena in Electrocoagulation Process: A Myth or a Reality? Desalination and Water Treatment, 51, 7536-7554.
 Zhang, Y., Zhuang, Y., Geng, J., Ren, H., Zhang, Y., Ding, L. and Xu, K. (2015) Inactivation of Antibiotic Resistance Genes in Municipal Wastewater Effluent by Chlorination and Sequential UV/Chlorination Disinfection. Science of the Total Environment, 512-513, 125-132.
 Ghernaout, D., Alghamdi, A., Aichouni, M. and Touahmia, M. (2018) The Lethal Water Tri-Therapy: Chlorine, Alum, and Polyelectrolyte. World Journal of Applied Chemistry, 3, 65-71.
 Boucherit, A., Moulay, S., Ghernaout, D., Al-Ghonamy, A.I., Ghernaout, B., Naceur, M.W., Ait Messaoudene, N., Aichouni, M., Mahjoubi, A.A. and Elboughdiri, N.A. (2015) New Trends in Disinfection By-Products Formation upon Water Treatment. Journal of Research & Developments in Chemistry, 2015, Article ID: 628833.
 Oh, J., Medriano, C.A. and Kim, S. (2016) The Effect of Tetracycline in the Antibiotic Resistance Gene Transfer before and after Ozone Disinfection. Desalination and Water Treatment, 57, 646-650. https://doi.org/10.1080/19443994.2014.986828
 Zheng, J., Su, C., Zhou, J.W., Xu, K., Qian, Y.Y. and Chen, H. (2017) Effects and Mechanisms of Ultraviolet, Chlorination, and Ozone Disinfection on Antibiotic Resistance Genes in Secondary Effluents of Municipal Wastewater Treatment Plants. Chemical Engineering Journal, 317, 309-316. https://doi.org/10.1016/j.cej.2017.02.076
 Guo, M.T., Yuan, Q.B. and Yang, J. (2015) Distinguishing Effects of Ultraviolet Exposure and Chlorination on the Horizontal Transfer of Antibiotic Resistance Genes in Municipal Wastewater. Environmental Science & Technology, 49, 5771-5778. https://doi.org/10.1021/acs.est.5b00644
 Huang, X., Liu, C.X., Li, K., Su, J.Q., Zhu, G.F. and Liu, L. (2015) Performance of Vertical Up-Flow Constructed Wetlands on Swine Wastewater Containing Tetracyclines and Tet Genes. Water Research, 70, 109-117. https://doi.org/10.1016/j.watres.2014.11.048
 Ghernaout, D. and Elboughdiri, N. (2020) Strategies for Reducing Disinfection By-Products Formation during Electrocoagulation. Open Access Library Journal, 7, e6076.
 Ghernaout, D. and Elboughdiri, N. (2020) Controlling Disinfection By-Products Formation in Rainwater: Technologies and Trends. Open Access Library Journal, 7, e6162.
 Ghernaout, D., Elboughdiri, N., Ghareba, S. and Salih, A. (2020) Disinfecting Water with the Carbon Fiber-Based Flow-through Electrode System (FES): towards Axial Dispersion and Velocity Profile. Open Access Library Journal, 7, e6238. https://doi.org/10.4236/oalib.1106238
 Zhang, Y., Yao, Z., Geng, J., Ren, H., Xu, K. and Ding, L. (2016) Reduction of Antibiotic Resistance Genes in Municipal Wastewater Effluent by Advanced Oxidation Processes. Science of the Total Environment, 50, 184-191. https://doi.org/10.1016/j.scitotenv.2016.01.078
 Yoon, Y., Chung, H.J., Wen, D.D., Dodd, M.C., Hur, H.G. and Lee, Y. (2017) Inactivation Efficiency of Plasmid-Encoded Antibiotic Resistance Genes during Water Treatment with Chlorine, UV, and UV/H2O2. Water Research, 123, 783-793.
 Guo, C.S., Wang, K., Hou, S., Wan, L., Lv, J.P., Zhang, Y., Qu, X.D., Chen, S.Y. and Xu, J. (2017) H2O2 and/Or TiO2 Photocatalysis under UV Irradiation for the Removal of Antibiotic Resistant Bacteria and Their Antibiotic Resistance Genes. Journal of Hazardous Materials, 323, 710-718. https://doi.org/10.1016/j.jhazmat.2016.10.041
 Ghernaout, D., Elboughdiri, N., Alghamdi, A. and Ghernaout, B. (2020) Trends in Decreasing Disinfection By-Products Formation during Electrochemical Technologies. Open Access Library Journal, 7, e6337. https://doi.org/10.4236/oalib.1106337
 Ghernaout, D., Badis, A., Ghernaout, B. and Kellil, A. (2008) Application of Electrocoagulation in Escherichia coli Culture and Two Surface Waters. Desalination, 219, 118-125.
 Ghernaout, D. (2019) Virus Removal by Electrocoagulation and Electrooxidation: New Findings and Future Trends. Journal of Environmental Science and Allied Research, 85-90.
 Ghernaout, D., Benblidia, C. and Khemici, F. (2015) Microalgae Removal from Ghrib Dam (Ain Defla, Algeria) Water by Electroflotation Using Stainless Steel Electrodes. Desalination and Water Treatment, 54, 3328-3337. https://doi.org/10.1080/19443994.2014.907749
 Rahmani, A.R., Samarghandi, M.R., Nematollahi, D. and Zamani, F. (2019) A Comprehensive Study of Electrochemical Disinfection of Water Using Direct and Indirect Oxidation Processes. Journal of Environmental Chemical Engineering, 7, Article ID: 102785.
 Di Cesare, A., De Carluccio, M., Eckert, E.M., Fontaneto, D., Fiorentino, A., Corno, G., Prete, P., Cucciniello, R., Proto, A. and Rizzo, L. (2020) Combination of Flow Cytometry and Molecular Analysis to Monitor the Effect of UVC/H2O2 Vs UVC/H2O2/Cu-IDS Processes on Pathogens and Antibiotic Resistant Genes in Secondary Wastewater Effluents. Water Research, 184, Article ID: 116194. https://doi.org/10.1016/j.watres.2020.116194
 Li, H., Zhang, S., Yang, X.-L., Xu, H., Yang, Y.-L., Wang, Y.-W. and Song, H.-L. (2018) Simulated Wastewater Reduced Klebsiella michiganensis Strain LH-2 Viability and Corresponding Antibiotic Resistance Gene Abundance in Bio-Electrochemical Reactors. Ecotoxicology and Environmental Safety, 162, 376-382. https://doi.org/10.1016/j.ecoenv.2018.07.018
 Guo, N., Wang, Y., Tong, T. and Wang, S. (2018) The Fate of Antibiotic Resistance Genes and Their Potential Hosts during Bio-Electrochemical Treatment of High- Salinity Pharmaceutical Wastewater. Water Research, 133, 79-86. https://doi.org/10.1016/j.watres.2018.01.020
 Yan, W., Xiao, Y., Yan, W., Ding, R., Wang, S. and Zhao, F. (2019) The Effect of Bioelectrochemical Systems on Antibiotics Removal and Antibiotic Resistance Genes: A Review. Chemical Engineering Journal, 358, 1421-1437. https://doi.org/10.1016/j.cej.2018.10.128
 Ghernaout, D., Ghernaout, B., Saiba, A., Boucherit, A. and, Kellil, A. (2009) Removal of Humic Acids by Continuous Electromagnetic Treatment Followed by Electrocoagulation in Batch Using Aluminium Electrodes. Desalination, 239, 295-308. https://doi.org/10.1016/j.desal.2008.04.001
 Ghernaout, D., Ghernaout, B. and Boucherit, A. (2008) Effect of pH on Electrocoagulation of Bentonite Suspensions in Batch Using Iron Electrodes. Journal of Dispersion Science and Technology, 29, 1272-1275. https://doi.org/10.1080/01932690701857483
 Ghernaout, D., Ghernaout, B. and Kellil, A. (2009) Natural Organic Matter Removal and Enhanced Coagulation as a Link between Coagulation and Electrocoagulation, Desalination and Water Treatment, 2, 203-222. https://doi.org/10.5004/dwt.2009.116
 Ghernaout, D., Ghernaout, B., Boucherit, A., Naceur, M.W., Khelifa, A. and Kellil, A. (2009) Study on Mechanism of Electrocoagulation with Iron Electrodes in Idealised Conditions and Electrocoagulation of Humic Acids Solution in Batch Using Aluminium Electrodes. Desalination and Water Treatment, 8, 91-99. https://doi.org/10.5004/dwt.2009.668
 Saiba, A., Kourdali, S., Ghernaout, B. and Ghernaout, D. (2010) In Desalination, from 1987 to 2009, the Birth of a New Seawater Pretreatment Process: Electrocoagulation—An Overview. Desalination and Water Treatment, 16, 201-217. https://doi.org/10.5004/dwt.2010.1094
 Belhout, D., Ghernaout, D., Djezzar-Douakh, S. and Kellil, A. (2010) Electrocoagulation of a Raw Water of Ghrib Dam (Algeria) in Batch Using Iron Electrodes. Desalination and Water Treatment, 16, 1-9. https://doi.org/10.5004/dwt.2010.1081
 Ghernaout, D., Mariche, A., Ghernaout, B. and Kellil, A. (2010) Electromagnetic Treatment-Bi-Electrocoagulation of Humic Acid in Continuous Mode Using Response Surface Method for Its Optimization and Application on Two Surface Waters. Desalination and Water Treatment, 22, 311-329. https://doi.org/10.5004/dwt.2010.1120
 Ghernaout, D. and Ghernaout, B. (2011) On the Controversial Effect of Sodium Sulphate as Supporting Electrolyte on Electrocoagulation Process: A Review. Desalination and Water Treatment, 27, 243-254. https://doi.org/10.5004/dwt.2011.1983
 Ghernaout, D., Naceur, M.W. and Ghernaout, B. (2011) A Review of Electrocoagulation as a Promising Coagulation Process for Improved Organic and inorganic Matters Removal by Electrophoresis and Electroflotation. Desalination and Water Treatment, 28, 287-320.
 Ghernaout, D., Irki, S. and Boucherit, A. (2014) Removal of Cu2+ and Cd2+, and Humic Acid and Phenol by Electrocoagulation Using Iron Electrodes. Desalination and Water Treatment, 52, 3256-3270. https://doi.org/10.1080/19443994.2013.852484
 Ghernaout, D., Al-Ghonamy, A.I., Naceur, M.W., Ait Messaoudene, N. and Aichouni, M. (2014) Influence of Operating Parameters on Electrocoagulation of C.I. Disperse Yellow 3. Journal of Electrochemical Science and Engineering, 4, 271-283. https://doi.org/10.5599/jese.2014.0065
 Ghernaout, D., Al-Ghonamy, A.I., Irki, S., Grini, A., Naceur, M.W., Ait Messaoudene, N. and Aichouni, M. (2014) Decolourization of Bromophenol Blue by Electrocoagulation Process. Trends in Chemical Engineering, 15, 29-39.
 Ghernaout, D., Al-Ghonamy, A.I., Ait Messaoudene, N., Aichouni, M., Naceur, M.W., Benchelighem, F.Z. and Boucherit, A. (2015) Electrocoagulation of Direct Brown 2 (DB) and BF Cibacete Blue (CB) Using Aluminum Electrodes. Separation Science and Technology, 50, 1413-1420. https://doi.org/10.1080/01496395.2014.982763
 Irki, S., Ghernaout, D. and Naceur, M.W. (2017) Decolourization of Methyl Orange (MO) by Electrocoagulation (EC) Using Iron Electrodes under a Magnetic Field (MF). Desalination and Water Treatment, 79, 368-377. https://doi.org/10.5004/dwt.2017.20797
 Ghernaout, D. (2008) élimination des substances humiques et des germes indicateurs de contamination bactériologique par électrocoagulation assistée d’un traitement magnétique de l’eau. Ph.D. Thesis, University of Blida, Algeria.
 Irki, S., Ghernaout, D., Naceur, M.W., Alghamdi, A. and Aichouni, M. (2018) Decolorization of Methyl Orange (MO) by Electrocoagulation (EC) Using Iron Electrodes under a Magnetic Field (MF). II. Effect of Connection Mode. World Journal of Applied Chemistry, 3, 56-64.
 Irki, S., Ghernaout, D., Naceur, M.W., Alghamdi, A. and Aichouni, M. (2018) Decolorizing Methyl Orange by Fe-Electrocoagulation Process—A Mechanistic Insight. International Journal of Environmental Chemistry, 2, 18-28. https://doi.org/10.11648/j.ijec.20180201.14
 Ghernaout, D., Alghamdi, A. and Ghernaout, B. (2019) Electrocoagulation Process: A Mechanistic Review at the Dawn of Its Modeling. Journal of Environmental Science and Allied Research, 2, 51-67. https://doi.org/10.29199/2637-7063/ESAR-201019
 Ghernaout, D., Elboughdiri, N. and Alghamdi, A. (2019) Direct Potable Reuse: the Singapore NEWater Project as a Role Model. Open Access Library Journal, 6, e5980.
 Ma, X., Guo, N., Ren, S., Wang, S. and Wang, Y. (2019) Response of Antibiotic Resistance to the Co-Existence of Chloramphenicol and Copper during Bio-Electrochemical Treatment of Antibiotic-Containing Wastewater. Environment International, 126, 127-133.
 Li, H., Zhang, Z., Duan, J., Li, N., Li, B., Song, T., Sardar, M.F., Lv, X. and Zhu, C. (2020) Electrochemical Disinfection of Secondary Effluent from a Wastewater Treatment Plant: Removal Efficiency of ARGs and Variation of Antibiotic Resistance in Surviving Bacteria. Chemical Engineering Journal, 392, Article ID: 123674. https://doi.org/10.1016/j.cej.2019.123674
 Zhang, S., Yang, Y.-L., Lu, J., Zuo, X.-J., Yang, X.-L. and Song, H.-L. (2020) A Review of Bioelectrochemical Systems for Antibiotic Removal: Efficient Antibiotic Removal and Dissemination of Antibiotic Resistance Genes. Journal of Water Process Engineering, 37, Article ID: 101421. https://doi.org/10.1016/j.jwpe.2020.101421
 Ghernaout, D. and Ghernaout, B. (2012) On the Concept of the Future Drinking Water Treatment Plant: Algae Harvesting from the Algal Biomass for Biodiesel Production—A Review. Desalination and Water Treatment, 49, 1-18. https://doi.org/10.1080/19443994.2012.708191
 Ghernaout, D. (2017) Water Reuse (WR): The Ultimate and Vital Solution for Water Supply Issues. International Journal of Sustainable Development Research, 3, 36-46.
 Ghernaout, D. (2018) Magnetic Field Generation in the Water Treatment Perspectives: An Overview. International Journal of Advanced and Applied Sciences, 5, 193-203.