AS  Vol.11 No.8 , August 2020
Field Efficacy of a Biopesticide Based on Tithonia diversifolia against Black Sigatoka Disease of Plantain (Musa spp., AAB)
Abstract: Black Sigatoka disease (BSD) is a foliar disease caused by Mycosphaerella fijiensis, responsible of reduction of the photosynthetic area of banana plant and yield at harvest since it has an influence on fruit physiology. The control of BSD relies on the use of chemicals which are not affordable for the small holder farmers and increase the cost of production. Moreover, this chemical control is ineffective, negatively impacting the environment and human health, and is at the origin of strain resistance. Tithonia diversifolia is known as rich in many compounds such as mineral elements, defense metabolites, some phytochemicals; and it is increasingly used in agriculture. Recently, the protective effect of Tithonia diversifolia liquid extract against BSD development on plantain vivoplants in the nursery was highlighted. The aim of our study was to evaluate the efficacy of a biopesticide base on Tithonia diversifolia on the BSD development in a plantain field under high disease pressure. The effect of Tithonia diversifolia biopesticide on Mycosphaerella fijiensis mycelial growth in vitro was evaluated. An experimental field at the flowering stage was selected and treated with the biopesticide base on Tithonia diversifolia at three different concentrations: undiluted (100%), diluted at 1/2 (50%) and diluted at 1/4 (25%) for 17 weeks. The disease severity, the number of functional leaves, the youngest spotted leaf (YSL) and the youngest necrotic leaf (YNL) were evaluated in course of time. The biopesticide treatments significantly (P < 0.001) reduce the BSD severity in course of time, but it is more effective for the most diluted concentration (25%). The number of leaves increases in course of time as well as the rank of the YSL and the YNL confirming the efficiency of BSD control. The efficacy of this biopesticide base on Tithonia diversifolia could be a hopeful ecoresponsible solution for the plantain sector in general and in particular for poor small farmers.
Cite this paper: Ewané, C. , Tatsegouock, R. , Meshuneke, A. and Niemenak, N. (2020) Field Efficacy of a Biopesticide Based on Tithonia diversifolia against Black Sigatoka Disease of Plantain (Musa spp., AAB). Agricultural Sciences, 11, 730-743. doi: 10.4236/as.2020.118048.

[1]   Jones, D.R.D. (2000) Diseases of Banana, Abaca and Enset. CABI Publication, Wallingford, 544 p.

[2]   Lassoudière, A. (2007) Le bananier et sa culture. Editions Quae, 383 p.

[3]   Ewané, C.A., Chillet, M., Castelan, F., Brostaux, Y., Lassois, L., Ngando, E.J., Hubert, O., Chilin-Charles, Y., Lepoivre, P. and de Lapeyre de Bellaire, L. (2013) Impact of the Extension of Black Leaf Streak Disease on Banana Susceptibility to Post-Harvest Diseases. Fruits, 68, 351-365.

[4]   Onautshu, O.D. (2013) Caractérisation des populations de Mycosphaerella fijiensis et épidémiologie de la cercosporiose noire du bananier (Musa spp.) dans la région de Kisangani-République Démocratique du Congo. Thèse de doctorat ès science, Université Catholique de Louvain.

[5]   Fouré, E. and Lescot, T. (1988) Variabilité génétique des Mycosphaerella inféodés au genre Musa. Mise en évidence de la présence au Cameroun sur bananier et plantain d’une cercosporiose (Mycosphaerella musicola) au comportement pathogène atypique, Fruits, 43, 407-415.

[6]   Gowen, S. (2012) Bananas and Plantains. Springer Science & Business Media, Berlin, 612 p.

[7]   Bennett, R.S. and Arneson, P.A. (2003) Black Sigatoka. The Plant Health Instructor.

[8]   Oluma, H.O.A., Onekutu, A. and Onyezili, F.N. (2004) Reactions of Plantain and Banana Cultivars to Black Sigatoka Leaf Spot Disease in Three Farming Systems in the Nigerian Guinea Savanna. Journal of Plant Diseases and Protection, 111, 158-164.

[9]   de Lapeyre de Bellaire, L., Fouré, E., Abadie, C. and Carlier, J. (2010) Black Leaf Streak Disease Is Challenging the Banana Industry. Fruits, 65, 327-342.

[10]   Oyerinde, R.O., Otusanya, O.O. and Akpor, O.B. (2009) Allelopathic Effect of Tithonya diversifolia on the Germination, Growth and Cholorophyl of Maize (Zea mays L.). Scientific Research and Essay, 4, 879-888.

[11]   Kaho, F., Yemefack, M., Feudjio-Teguefouet, P. and Tchantchouang, J.C. (2011) Effet combiné des feuilles de Tithonya diversifolia et des engrais inorganiques sur les rendements du maïs et les propriétés d’un sol ferralitique au Centre Cameroun. Tropicultura, 29, 39-45.

[12]   Kerebba, N., Oyedeji, A.O., Byamukama, R., Kuria, S.K. and Oyedeji, O.O. (2019) Pesticidal Activity of Tithonia diversifolia (Hemsl.) A. Gray and Tephrosia vogelii (Hook f.); Phytochemical Isolation and Characterization: A Review. South African Journal of Botany, 121, 366-376.

[13]   Kandungu, J., Anjarwalla, P., Mwaura, L., Ofori, D.A., Jammadass, R., Stevenson, P.C. and Smith, P. (2013) “Pesticidal Plant Leaflet” Tithonya diversifolia (Hemsley) A. Gray. Kew Royal Botanic Gardens, World Agroforestry Centre.

[14]   Diby, Y.K.S., Tahiri, Y.A., Akpesse, A.A.M., Trabi, C.S. and Kouassi, K.P. (2015) Evaluation de l’effet insecticide de l’extrait aqueux de Tithonia diversifolia (Hemsl.) gray (Asteracee) sur les termites en culture du riz (NERICA 1) au centre de la Cote d’Ivoire. Journal of Animal & Plant Sciences, 25, 3966-3976.

[15]   Tatsegouock, R.N., Ewane, C.A., Meshuneke, A. and Boudjeko, T. (2020) Plantain Bananas PIF Seedlings Treatment with Liquid Extract of Tithonia diversifolia Induces Resistance to Black Sigatoka Disease. American Journal of Plant Science, 11, 653-671.

[16]   Dihazi, A. (2012) Interaction palmier dattier-fusarium oxyporium f. sp albedinis: Induction des réactions de défense par l’acide salicylique et rôle de quelques microorganismes antagonistes de l’agent pathogènes dans le contrôle de la maladie de Bayoud. Thèse présentée en vue de l’obtention d’un Doctorat National. Laboratoire de biotechnologie et de valorisation des ressources naturelles, Faculté des sciences, Agadir.

[17]   Gauhl, F., Pasberg-Gauhl, F., Vuylsteke, D. and Ortiz, R. (1993) Multilocational Evaluation of Black Sigatoka Resistance in Banana and Plantain, IITA, IITA Res. Guide No. 47, Ibadan, 59 p.

[18]   Stover, R.H. and Dickson, J.D. (1976) Banana Leaf Spot Caused by Mycosphaerella musicola and M. fijiensis var. Difformis: A Comparison of the First Central American Epidemics. FAO Plant Protection Bulletin, 24, 36-42.

[19]   Addinsoft (2020) XLSTAT Statistical and Data Analysis Solution. New York.

[20]   Hemsley, A. and Gray (2005) Allelopathic Effects of Mexican Sunflower Tithonia diversifolia on Germination and Growth of Spider Plant (Cleome gynandra L.). Journal of Biodiversity and Environmental Sciences, 2, 26-35.

[21]   Umar, O.B., Alex, R.D. and Obukohwo, E.E. (2015) Phytochemical and Proximate Composition of Tithonia diversifolia (Hemsl.) A. Gray. Annals Food Science and Technology, 16, 195-200.

[22]   Muirhead, I.F. and Jones, D.R. (2000) Fungal Diseases of the Foliage. Sigatoka Leaf Spots. In: Jones, D.R., Ed., Diseases of Banana, Abaca and Ensete, CABI Publication, Wallingford, 544 p.

[23]   Abadie, C., Hubert, O., Ngando, J.E., Ngoh, G., Mbeguie-A-Mbeguie, D., de Lapeyre de Bellaire, L. and Chillet, M. (2008) Evidence of the Effects of Mycosphaerella Leaf Spot Diseases on Fruit Quality. Mem. XVIII ACORBAT Meeting, Guayaquil, Ecuador, ACORBAT International, 8 p.

[24]   Chillet, M., Abadie, C., Hubert, O., Chilin-Charles, Y. and de Lapeyre de Bellaire, L. (2009) Sigatoka Disease Reduces the Greenlife of Bananas. Crop Protection, 26, 1078-1082.

[25]   Ewané, C.A., Lassois, L., Brostaux, Y., Lepoivre, P. and de Lapeyre de Bellaire, L. (2013) The Susceptibility of Bananas to Crown Rot Disease Is Influenced by Geographic and Temporal Effects. Canadian Journal of Plant Pathology, 35, 27-36.

[26]   Mur, L.A.J., Simpson, C., Kumari, A., Gupta, A.K. and Gupta, K.J. (2016) Moving Nitrogen to the Centre of Plant Defence against Pathogens. Annals of Botany, 119, 703-709.

[27]   Ewané, C.A., Ange, M.C., Felix, N.E. and Boudjeko, T. (2020) Influence of Clam Shells and Tithonia diversifolia Powder on Growth of Plantain PIF Seedlings (var. French) and Their Sensitivity to Mycosphaerella fijiensis. African Journal of Agricultural Research, 15, 393-411.

[28]   Ewané, C.A., Meshuneke, A., Tatsegouock, R.N. and Boudjeko, T. (2020) Vertical Layers of Tithonia diversifolia Flakes Amendment Improves Plantain Seedling Performance. American Journal of Agricultural Research, 5, 95.

[29]   Meshuneke, A., Ewané, C.A., Tatsegouock, R.N. and Boudjeko, T. (2020) Tithonia diversifolia Mulch Stimulates the Growth of Plantain PIF Seedlings and Induces a Less Susceptibility to Mycosphaerella fijiensis in the Nursery. American Journal of Plant Science, 11, 672-692.